| Literature DB >> 30267350 |
Abolade A Oyelade1,2, Olawale Olufemi Adelowo3, Obasola Ezekiel Fagade3.
Abstract
Twenty-six strains of Vibrio parahaemolyticus and 14 strains of V. vulnificus isolated from selected beaches in Lagos State, Nigeria, were examined for virulence and antimicrobial resistance genes. The V. parahaemolyticus isolates were further serotyped and subjected to pulsed field gel electrophoresis (PFGE). Five strains of V. vulnificus and one of V. parahaemolyticus carried the New Delhi-metallo-beta-lactamase gene blaNDM-1, seven strains carried blaTEM, and four strains of V. vulnificus and one of V. parahaemolyticus carried blaCMY. Real-time PCR assay for detection of virulence genes tdh and trh in the V. parahaemolyticus isolates showed that five isolates were positive for tdh, two for trh, and one isolate carried both genes. Ten V. parahaemolyticus serogroups and 23 pulsotypes were identified from 26 isolates based on O and K antigens typing and PFGE. Five of the isolates belong to the pandemic strains O1:Kut and O3:K6, and three belonged to the highly virulent O4:Kut serotype. Nineteen of the isolates showed distinct PFGE banding patterns. These results highlighted the importance of Nigerian recreational beaches as reservoirs of antimicrobial resistance genes of global public health interest, such as blaNDM-1.Entities:
Keywords: Recreational beaches; V. parahaemolyticus; V. vulnificus; bla CMY; bla NDM-1; bla TEM; tdh; trh
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Year: 2018 PMID： 30267350 DOI： 10.1007/s11356-018-3306-2
Source DB: PubMed Journal: Environ Sci Pollut Res Int ISSN： 0944-1344 Impact factor: 4.223