Literature DB >> 3025616

Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.

R W Mercer, J W Schneider, A Savitz, J Emanuel, E J Benz, R Levenson.   

Abstract

We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.

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Year:  1986        PMID： 3025616      PMCID： PMC367151          DOI： 10.1128/mcb.6.11.3884-3890.1986

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  24 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Amplification of DNA sequences coding for the Na,K-ATPase alpha-subunit in ouabain-resistant C+ cells.

Authors:  J R Emanuel; S Garetz; J Schneider; J F Ash; E J Benz; R Levenson
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

Review 3.  Empirical predictions of protein conformation.

Authors:  P Y Chou; G D Fasman
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

4.  Molecular considerations relevant to the mechanism of active transport.

Authors:  J Kyte
Journal:  Nature       Date:  1981-07-16       Impact factor: 49.962

5.  Ouabain-binding-site photoaffinity probes that label both subunits of Na+,K+-ATPase.

Authors:  C Hall; A Ruoho
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

Review 6.  Does Na+-K+-atpase have any role in bile secretion?

Authors:  S Erlinger
Journal:  Am J Physiol       Date:  1982-10

7.  A simple method for displaying the hydropathic character of a protein.

Authors:  J Kyte; R F Doolittle
Journal:  J Mol Biol       Date:  1982-05-05       Impact factor: 5.469

8.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  33 in total

1.  Identification of thyroid regulatory elements in the Na-K-ATPase alpha3 gene promoter.

Authors:  M Bajpai; S K Mandal; S Chaudhury
Journal:  Mol Biol Rep       Date:  2001-03       Impact factor: 2.316

2.  Expression and developmental regulation of Na+,K+ adenosine triphosphatase in the rat small intestine.

Authors:  B V Zemelman; W A Walker; S H Chu
Journal:  J Clin Invest       Date:  1992-09       Impact factor: 14.808

3.  Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture.

Authors:  G Pietrini; M Matteoli; G Banker; M J Caplan
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

4.  Chromosome-mediated transfer of the murine Na,K-ATPase alpha subunit confers ouabain resistance.

Authors:  D Fallows; R B Kent; D L Nelson; J R Emanuel; R Levenson; D E Housman
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

5.  Cell-specific expression of mRNAs encoding Na+,K(+)-ATPase alpha- and beta-subunit isoforms within the rat central nervous system.

Authors:  A G Watts; G Sanchez-Watts; J R Emanuel; R Levenson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

6.  Structural organization and transcription of the mouse gastric H+, K(+)-ATPase beta subunit gene.

Authors:  V A Canfield; R Levenson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-15       Impact factor: 11.205

Review 7.  Subunit assembly and functional maturation of Na,K-ATPase.

Authors:  K Geering
Journal:  J Membr Biol       Date:  1990-05       Impact factor: 1.843

8.  The gamma subunit of the Na,K-ATPase induces cation channel activity.

Authors:  N T Minor; Q Sha; C G Nichols; R W Mercer
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

9.  Isoforms of Na,K-ATPase alpha and beta subunits in the rat cerebellum and in granule cell cultures.

Authors:  L Peng; P Martin-Vasallo; K J Sweadner
Journal:  J Neurosci       Date:  1997-05-15       Impact factor: 6.167

10.  Identification of a region within the Na,K-ATPase alpha subunit that contributes to differential ouabain sensitivity.

Authors:  J R Emanuel; S Graw; D Housman; R Levenson
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272
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