Literature DB >> 30242700

Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays.

Lori Buetow1,2, Mads Gabrielsen1,2, Danny T Huang3,4.   

Abstract

RING and U-box ubiquitin ligases promote ubiquitin (Ub) transfer by priming Ub-conjugated E2 in a closed conformation to optimize the thioester bond for nucleophilic attack by substrate lysine. Here, we describe a single-turnover lysine discharge assay for direct assessment of the activity of any RING/U-box E3-E2~Ub complex.

Entities:  

Keywords:  Lysine discharge; RING or U-box E3 ligases; Radiolabeling; Ubiquitination

Mesh：

Substances：

Year:  2018        PMID： 30242700      PMCID： PMC6847041          DOI： 10.1007/978-1-4939-8706-1_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

1.  Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex.

Authors:  Peter S Brzovic; Jennifer R Keeffe; Hiroyuki Nishikawa; Keiko Miyamoto; David Fox; Mamoru Fukuda; Tomohiko Ohta; Rachel Klevit
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-05       Impact factor: 11.205

2.  Ubiquitin transfer from the E2 perspective: why is UbcH5 so promiscuous?

Authors:  Peter S Brzovic; Rachel E Klevit
Journal:  Cell Cycle       Date:  2006-12-08       Impact factor: 4.534

3.  A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination.

Authors:  Peter S Brzovic; Alexei Lissounov; Devin E Christensen; David W Hoyt; Rachel E Klevit
Journal:  Mol Cell       Date:  2006-03-17       Impact factor: 17.970

4.  Structural mechanisms underlying posttranslational modification by ubiquitin-like proteins.

Authors:  Billy T Dye; Brenda A Schulman
Journal:  Annu Rev Biophys Biomol Struct       Date:  2007

Review 5.  Structural insights into the catalysis and regulation of E3 ubiquitin ligases.

Authors:  Lori Buetow; Danny T Huang
Journal:  Nat Rev Mol Cell Biol       Date:  2016-08-03       Impact factor: 94.444

Review 6.  The ubiquitin code.

Authors:  David Komander; Michael Rape
Journal:  Annu Rev Biochem       Date:  2012-04-10       Impact factor: 23.643

7.  BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer.

Authors:  Hao Dou; Lori Buetow; Gary J Sibbet; Kenneth Cameron; Danny T Huang
Journal:  Nat Struct Mol Biol       Date:  2012-08-14       Impact factor: 15.369

8.  UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.

Authors:  Dawn M Wenzel; Alexei Lissounov; Peter S Brzovic; Rachel E Klevit
Journal:  Nature       Date:  2011-05-01       Impact factor: 49.962

9.  Intrinsic disorder drives N-terminal ubiquitination by Ube2w.

Authors:  Vinayak Vittal; Lei Shi; Dawn M Wenzel; K Matthew Scaglione; Emily D Duncan; Venkatesha Basrur; Kojo S J Elenitoba-Johnson; David Baker; Henry L Paulson; Peter S Brzovic; Rachel E Klevit
Journal:  Nat Chem Biol       Date:  2014-12-01       Impact factor: 15.040

10.  Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8.

Authors:  Daniel C Scott; Vladislav O Sviderskiy; Julie K Monda; John R Lydeard; Shein Ei Cho; J Wade Harper; Brenda A Schulman
Journal:  Cell       Date:  2014-06-19       Impact factor: 41.582
View more
  3 in total

Review 1.  The BRCA1/BARD1 ubiquitin ligase and its substrates.

Authors:  Samuel R Witus; Mikaela D Stewart; Rachel E Klevit
Journal:  Biochem J       Date:  2021-09-30       Impact factor: 3.857

2.  A Comprehensive Assessment of Genetic and Epigenetic Alterations Identifies Frequent Variations Impacting Six Prototypic SCF Complex Members.

Authors:  Rubi Campos Gudiño; Ally C Farrell; Nicole M Neudorf; Kirk J McManus
Journal:  Int J Mol Sci       Date:  2021-12-22       Impact factor: 5.923

3.  A High-Throughput Assay for Monitoring Ubiquitination in Real Time.

Authors:  Tyler G Franklin; Jonathan N Pruneda
Journal:  Front Chem       Date:  2019-12-04       Impact factor: 5.221
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.