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Literature DB >> 3023616

A new strategy for mapping the human genome.

Abstract

Recent advances in agarose gel electrophoresis of large DNA fragments raise the possibility of an entirely new approach to mapping mammalian genomes. In this article is discussed the potential of this technology for tackling problems such as construction of linkage maps, identifying chromosome translocation breakpoints, and moving from linked markers to genes causing diseases such as the muscular dystrophies and Huntington's chorea.

Entities: Chemical Disease Species

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Year: 1986 PMID： 3023616 PMCID： PMC1049779 DOI： 10.1136/jmg.23.5.421

Source DB: PubMed Journal: J Med Genet ISSN： 0022-2593 Impact factor: 6.318

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References

14 in total

1. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors: G F Carle; M V Olson
Journal: Nucleic Acids Res Date: 1984-07-25 Impact factor: 16.971

2. A polymorphic DNA marker genetically linked to Huntington's disease.

Authors: J F Gusella; N S Wexler; P M Conneally; S L Naylor; M A Anderson; R E Tanzi; P C Watkins; K Ottina; M R Wallace; A Y Sakaguchi
Journal: Nature Date: 1983 Nov 17-23 Impact factor: 49.962

3. Optimal use of restriction enzymes in the analysis of human DNA polymorphism.

Authors: F Bastié-Sigeac; G Lucotte
Journal: Hum Genet Date: 1983 Impact factor: 4.132

4. Linkage relationship of a cloned DNA sequence on the short arm of the X chromosome to Duchenne muscular dystrophy.

Authors: J M Murray; K E Davies; P S Harper; L Meredith; C R Mueller; R Williamson
Journal: Nature Date: 1982-11-04 Impact factor: 49.962

5. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

6. Localisation of the Becker muscular dystrophy gene on the short arm of the X chromosome by linkage to cloned DNA sequences.

Authors: H M Kingston; M Sarfarazi; N S Thomas; P S Harper
Journal: Hum Genet Date: 1984 Impact factor: 4.132

A new strategy for mapping the human genome.

1. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

2. A polymorphic DNA marker genetically linked to Huntington's disease.

3. Optimal use of restriction enzymes in the analysis of human DNA polymorphism.

4. Linkage relationship of a cloned DNA sequence on the short arm of the X chromosome to Duchenne muscular dystrophy.

5. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

6. Localisation of the Becker muscular dystrophy gene on the short arm of the X chromosome by linkage to cloned DNA sequences.

7. Osteosarcoma and retinoblastoma: a shared chromosomal mechanism revealing recessive predisposition.

8. Localization of cystic fibrosis locus to human chromosome 7cen-q22.

9. Detection of deletions spanning the Duchenne muscular dystrophy locus using a tightly linked DNA segment.

10. The apolipoprotein CII gene: subchromosomal localisation and linkage to the myotonic dystrophy locus.