Literature DB >> 30220493

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes.

Mathilde Lescat1, Laurent Poirel2, Patrice Nordmann3.   

Abstract

A rapid (total time <2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a single isolate possessing both mcr-1 and mcr-5 genes.
Copyright © 2018. Published by Elsevier Inc.

Keywords:  Multiplex PCR; Polymyxin resistance6; mcr genes

Mesh：

Substances：

Year:  2018        PMID： 30220493     DOI： 10.1016/j.diagmicrobio.2018.04.010

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  18 in total

1.  A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa.

Authors:  Mathilde Lescat; Laurent Poirel; Camille Tinguely; Patrice Nordmann
Journal:  J Clin Microbiol       Date:  2019-02-27       Impact factor: 5.948

2.  Functional Characterization of a Miniature Inverted Transposable Element at the Origin of mcr-5 Gene Acquisition in Escherichia coli.

Authors:  Nicolas Kieffer; Patrice Nordmann; Yves Millemann; Laurent Poirel
Journal:  Antimicrob Agents Chemother       Date:  2019-06-24       Impact factor: 5.191

3.  Screening and Characterization of Multidrug-Resistant Gram-Negative Bacteria from a Remote African Area, São Tomé and Príncipe.

Authors:  Laurent Poirel; Marta Aires-de-Sousa; Patrick Kudyba; Nicolas Kieffer; Patrice Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2018-08-27       Impact factor: 5.191

4.  Rapid detection of human origin colistin-resistance genes mcr-1, mcr-3, mcr-8, mcr-10 in clinical fecal samples.

Authors:  Shuangfang Hu; Ziquan Lv; Yang Wang; Jianzhong Shen; Yuebin Ke
Journal:  Arch Microbiol       Date:  2021-06-14       Impact factor: 2.552

5.  Phenotypic Detection of Plasmid-Mediated Colistin Resistance in Enterobacteriaceae.

Authors:  Jose A Di Conza; Gabriel O Gutkind; Edgar Gonzales Escalante; Katherine Yauri Condor
Journal:  J Clin Microbiol       Date:  2020-02-24       Impact factor: 5.948

6.  Effects of Dietary Antimicrobial Growth Promoters on Performance Parameters and Abundance and Diversity of Broiler Chicken Gut Microbiome and Selection of Antibiotic Resistance Genes.

Authors:  Shyam Sundar Paul; Savaram Venkata Rama Rao; Nagendra Hegde; Nicola J Williams; Rudra Nath Chatterjee; Mantena Venkata Lakshmi Narasimha Raju; Godumagadda Narender Reddy; Vikas Kumar; Prakki Santosh Phani Kumar; Sathi Mallick; Madhuranjana Gargi
Journal:  Front Microbiol       Date:  2022-06-16       Impact factor: 6.064

7.  Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae.

Authors:  Hervé Volland; Laurent Dortet; Sandrine Bernabeu; Hervé Boutal; Marisa Haenni; Jean-Yves Madec; Frédéric Robin; Racha Beyrouthy; Thierry Naas; Stéphanie Simon
Journal:  J Clin Microbiol       Date:  2019-04-26       Impact factor: 5.948

8.  Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods.

Authors:  John Osei Sekyere
Journal:  Microbiologyopen       Date:  2018-07-04       Impact factor: 3.139

9.  Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products.

Authors:  Tereza Gelbíčová; Alžběta Baráková; Martina Florianová; Ivana Jamborová; Markéta Zelendová; Lucie Pospíšilová; Ivana Koláčková; Renáta Karpíšková
Journal:  Front Microbiol       Date:  2019-12-12       Impact factor: 5.640

10.  Epidemiology of Carbapenemase-Producing Klebsiella pneumoniae in a Hospital, Portugal.

Authors:  Marta Aires-de-Sousa; José Manuel Ortiz de la Rosa; Maria Luísa Gonçalves; Ana Luísa Pereira; Patrice Nordmann; Laurent Poirel
Journal:  Emerg Infect Dis       Date:  2019-09       Impact factor: 6.883
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