| Literature DB >> 30567745 |
Mathilde Lescat1,2,3,4, Laurent Poirel1,2,5, Camille Tinguely1,2,5, Patrice Nordmann6,2,5,7.
Abstract
A rapid test was developed for identification of polymyxin resistance in nonfermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, as observed by its color change (blue to purple or pink). Its evaluation was performed by using 92 colistin-resistant and colistin-susceptible Acinetobacter baumannii and Pseudomonas aeruginosa isolates. Sensitivity and specificity were found to be 100% and 95%, respectively, by comparison with the standard broth microdilution method. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is inexpensive, easy to perform, highly sensitive and specific, and can be completed in 4 hours. It could be useful in countries facing endemic spread of colistin-resistant nonfermenters.Entities:
Keywords: Acinetobacter baumanniizzm321990; Pseudomonas aeruginosa; colistin; rapid diagnostic test; susceptibility testing
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Year: 2019 PMID： 30567745 PMCID： PMC6425163 DOI： 10.1128/JCM.01563-18
Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948