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Literature DB >> 3022011

Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters.

Abstract

We cloned the intact T4 rIIB gene by joining plasmids carrying gene fragments. rIIB was expressed at a low level under control of the lac promoter, and the clone complemented rIIB mutants. We suspect that earlier attempts to clone the intact gene were unsuccessful because of transcription from T4 middle-mode promoters. These promoters are silent early in infection but are recognized when resident on a plasmid in an uninfected cell.

Entities: Disease Species

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1986 PMID： 3022011 PMCID： PMC288959

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

15 in total

1. Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.

Authors: H L Yang; K Heller; M Gellert; G Zubay
Journal: Proc Natl Acad Sci U S A Date: 1979-07 Impact factor: 11.205

2. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.

Authors: R C Parker; R M Watson; J Vinograd
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

3. A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4.

Authors: B S Singer; L Gold
Journal: J Mol Biol Date: 1976-05-25 Impact factor: 5.469

4. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.

Authors: C L Smith; M Kubo; F Imamoto
Journal: Nature Date: 1978-10-05 Impact factor: 49.962

5. Intracellular DNA-protein complexes from bacteriophage T4-infected cells isolated by a rapid two-step procedure. Characterization and identification of the protein components.

Authors: C Manoil; N Sinha; B Alberts
Journal: J Biol Chem Date: 1977-04-25 Impact factor: 5.157

2. The rIIA gene of bacteriophage T4. I. Its DNA sequence and discovery of a new open reading frame between genes 60 and rIIA.

Authors: P Daegelen; E Brody
Journal: Genetics Date: 1990-06 Impact factor: 4.562

3. Mutations affecting translation of the bacteriophage T4 rIIB gene cloned in Escherichia coli.

Authors: S Shinedling; M Gayle; D Pribnow; L Gold
Journal: Mol Gen Genet Date: 1987-05

4. Wild-type bacteriophage T4 is restricted by the lambda rex genes.

Authors: S Shinedling; D Parma; L Gold
Journal: J Virol Date: 1987-12 Impact factor: 5.103

4 in total

Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters.

1. Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.

2. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.

3. A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4.

4. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.

5. Intracellular DNA-protein complexes from bacteriophage T4-infected cells isolated by a rapid two-step procedure. Characterization and identification of the protein components.

6. Mutant of bacteriophage T4D affecting expression of many early genes.

7. Realignment of the genetic map of the terminus of the rIIB cistron of bacteriophage T4.

8. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase.

9. Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4.

10. Altered Cro repressors from engineered mutagenesis of a synthetic cro gene.

1. Coordination of DNA ends during double-strand-break repair in bacteriophage T4.

2. The rIIA gene of bacteriophage T4. I. Its DNA sequence and discovery of a new open reading frame between genes 60 and rIIA.

3. Mutations affecting translation of the bacteriophage T4 rIIB gene cloned in Escherichia coli.

4. Wild-type bacteriophage T4 is restricted by the lambda rex genes.