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Literature DB >> 345100

Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4.

Abstract

The EcoRI digestion products of phage T4 DNA have been examined using a phage DNA transformation assay. A 2.6 X 10(6) Dalton fragment was found to contain the rII genes. This fragment was purified and then treated with HindIII endonuclease. The cleavage products were ligated to the vector plasmid pBR313 and viable recombinant plasmids recovered. A genetic assay was employed to demonstrate that the recombinants contained T4 DNA and to localize on the phage genetic map the EcoRI and HindIII sites cleaved during the construction of the plasmids. Preliminary characterization suggests that a fragment covering the beginning of the rIIA gene possibly contains a promotor which is active in uninfected cells.

Entities: Gene Species

Mesh：

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Year: 1978 PMID： 345100 DOI： 10.1007/bf00268267

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

22 in total

1. Mapping experiments with rII and b mutants of bacteriophage T4D.

Authors: R S EDGAR
Journal: Virology Date: 1958-08 Impact factor: 3.616

2. Characterization of a new class of deletions of the D region of the bacteriophage T4 genome.

Authors: R E Depew; T J Snopek; N R Cozzarelli
Journal: Virology Date: 1975-03 Impact factor: 3.616

3. ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE.

Authors: S Benzer
Journal: Proc Natl Acad Sci U S A Date: 1961-03 Impact factor: 11.205

4. The production of undegraded cytosine-containing DNA by bacteriophage T4 in the absence of dCTPase and endonucleases II and IV, and its effects on T4-directed protein synthesis.

Authors: E Kutter; A Beug; R Sluss; L Jensen; D Bradley
Journal: J Mol Biol Date: 1975-12-25 Impact factor: 5.469

5. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors: R B Helling; H M Goodman; H W Boyer
Journal: J Virol Date: 1974-11 Impact factor: 5.103

6. Transformationof phage T4 by small denatured DNA fragments.

Authors: H Zweerink; E B Goldberg
Journal: Virology Date: 1970-05 Impact factor: 3.616

7. Growth and transformation of phage T4 in Escherichia coli B-4, Salmonella, Aerobacter, Proteus, and Serratia.

Authors: A C Wais; E B Goldberg
Journal: Virology Date: 1969-10 Impact factor: 3.616

8. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

9. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors: W B Wood
Journal: J Mol Biol Date: 1966-03 Impact factor: 5.469

10. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes.

Authors: T Mattson; G Van Houwe; A Bolle; G Selzer; R Epstein
Journal: Mol Gen Genet Date: 1977-09-09

20 in total

1. The rIIA gene of bacteriophage T4. I. Its DNA sequence and discovery of a new open reading frame between genes 60 and rIIA.

Authors: P Daegelen; E Brody
Journal: Genetics Date: 1990-06 Impact factor: 4.562

Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4.

1. Mapping experiments with rII and b mutants of bacteriophage T4D.

2. Characterization of a new class of deletions of the D region of the bacteriophage T4 genome.

3. ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE.

4. The production of undegraded cytosine-containing DNA by bacteriophage T4 in the absence of dCTPase and endonucleases II and IV, and its effects on T4-directed protein synthesis.

5. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

6. Transformationof phage T4 by small denatured DNA fragments.

7. Growth and transformation of phage T4 in Escherichia coli B-4, Salmonella, Aerobacter, Proteus, and Serratia.

8. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

9. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

10. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes.

1. The rIIA gene of bacteriophage T4. I. Its DNA sequence and discovery of a new open reading frame between genes 60 and rIIA.

2. The 52-protein subunit of T4 DNA topoisomerase is homologous to the gyrA-protein of gyrase.

3. Nucleotide sequence of a type II DNA topoisomerase gene. Bacteriophage T4 gene 39.

4. Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters.

5. Realignment of the genetic map of the terminus of the rIIB cistron of bacteriophage T4.

6. Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4.

7. In vivo expression of the rII region of bacteriophage T4 present in chimeric plasmids.

8. Physical mapping of bacteriophage T4.

9. Bacteriophage T4 gol site: sequence analysis and effects of the site on plasmid transformation.

10. Simian virus 40 promoters direct expression of the tetracycline gene in plasmid pACYC184.