Literature DB >> 3021052

Molecular cloning of amikacin resistance determinants from a Klebsiella pneumoniae plasmid.

M E Tolmasky, M Roberts, M Woloj, J H Crosa.   

Abstract

A multiresistant Klebsiella pneumoniae strain, JHCK1, harbored several plasmids. One of these, plasmid pJHCMW1, carried determinants for resistance to amikacin in addition to kanamycin, tobramycin, and ampicillin. The amikacin resistance determinant(s) was cloned and studied by restriction mapping, insertion, and deletion analysis. The amikacin resistance gene(s) was localized in a 1.5-kilobase DNA fragment. This pJHCMW1 DNA region was responsible for not only the resistance to amikacin but also the resistance to kanamycin and tobramycin. The cloned DNA fragment specified both an acetyltransferase activity and a low level of phosphotransferase activity. The two activities were absent from mutants that did not confer resistance to amikacin, kanamycin, and tobramycin.

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Year:  1986        PMID： 3021052      PMCID： PMC180541          DOI： 10.1128/AAC.30.2.315

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  30 in total

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Authors:  B J Bachmann
Journal:  Bacteriol Rev       Date:  1972-12

2.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

3.  Antibiotic susceptibility testing by a standardized single disk method.

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Journal:  Am J Clin Pathol       Date:  1966-04       Impact factor: 2.493

4.  Aminoglycoside phosphotransferase-II-mediated amikacin resistance in Escherichia coli.

Authors:  G P Bongaerts; G M Kaptijn
Journal:  Antimicrob Agents Chemother       Date:  1981-09       Impact factor: 5.191

5.  Evidence for a chromosomal site specifying amikacin resistance in multiresistant Serratia marcescens.

Authors:  J F John; W F McNeill; K E Price; P A Kresel
Journal:  Antimicrob Agents Chemother       Date:  1982-04       Impact factor: 5.191

6.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

7.  The DNA-protein relaxation complex of the plasmid RK2: location of the site-specific nick in the region of the proposed origin of transfer.

Authors:  D G Guiney; D R Helinski
Journal:  Mol Gen Genet       Date:  1979-10-03

8.  Amikacin resistance associated with a plasmid-borne aminoglycoside phosphotransferase in Escherichia coli.

Authors:  M H Perlin; S A Lerner
Journal:  Antimicrob Agents Chemother       Date:  1979-11       Impact factor: 5.191

9.  In-vivo acquisition of two different types of aminoglycoside resistance by a single strain of Klebsiella pneumoniae causing severe infection.

Authors:  B E Murray; R C Moellering
Journal:  Ann Intern Med       Date:  1982-02       Impact factor: 25.391

10.  New plasmid-mediated aminoglycoside adenylyltransferase of broad substrate range that adenylylates amikacin.

Authors:  R G Coombe; A M George
Journal:  Antimicrob Agents Chemother       Date:  1981-07       Impact factor: 5.191
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  22 in total

1.  Cloning, sequencing, and use as a molecular probe of a gene encoding an aminoglycoside 6'-N-acetyltransferase of broad substrate profile.

Authors:  F J Terán; J E Suárez; M C Mendoza
Journal:  Antimicrob Agents Chemother       Date:  1991-04       Impact factor: 5.191

2.  Nosocomial spread of an amikacin resistance gene on both a mobilized, nonconjugative plasmid and a conjugative plasmid.

Authors:  J D Hopkins; A Flores; M del Pilar Pla; S Lester; T F O'Brien
Journal:  Antimicrob Agents Chemother       Date:  1991-08       Impact factor: 5.191

3.  Heterogeneity of 6'-N-acetyltransferases of type 4 conferring resistance to amikacin and related aminoglycosides in members of the family Enterobacteriaceae.

Authors:  G Tran Van Nhieu; E Collatz
Journal:  Antimicrob Agents Chemother       Date:  1988-08       Impact factor: 5.191

4.  Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae.

Authors:  K Nobuta; M E Tolmasky; L M Crosa; J H Crosa
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

5.  Primary structure of an aminoglycoside 6'-N-acetyltransferase AAC(6')-4, fused in vivo with the signal peptide of the Tn3-encoded beta-lactamase.

Authors:  G Tran van Nhieu; E Collatz
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

6.  Sublethal concentrations of the aminoglycoside amikacin interfere with cell division without affecting chromosome dynamics.

Authors:  Christophe Possoz; Jason Newmark; Nohemy Sorto; David J Sherratt; Marcelo E Tolmasky
Journal:  Antimicrob Agents Chemother       Date:  2006-10-16       Impact factor: 5.191

7.  Effects of F171 mutations in the 6'-N-acetyltransferase type Ib [AAC(6')-Ib] enzyme on susceptibility to aminoglycosides.

Authors:  R Chavideh; S Sholly; D Panaite; M E Tolmasky
Journal:  Antimicrob Agents Chemother       Date:  1999-11       Impact factor: 5.191

8.  Genetic analysis of the iron uptake region of the Vibrio anguillarum plasmid pJM1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis.

Authors:  M E Tolmasky; L A Actis; J H Crosa
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

9.  Tn1331, a novel multiresistance transposon encoding resistance to amikacin and ampicillin in Klebsiella pneumoniae.

Authors:  M E Tolmasky; J H Crosa
Journal:  Antimicrob Agents Chemother       Date:  1987-12       Impact factor: 5.191

10.  Inhibition of aminoglycoside 6'-N-acetyltransferase type Ib-mediated amikacin resistance by antisense oligodeoxynucleotides.

Authors:  Renee Sarno; Hongphuc Ha; Natalia Weinsetel; Marcelo E Tolmasky
Journal:  Antimicrob Agents Chemother       Date:  2003-10       Impact factor: 5.191
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