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Literature DB >> 3020368

Molecular cloning and expression in Escherichia coli of the structural gene for the hemolytic toxin aerolysin from Aeromonas hydrophila.

Abstract

The structural gene for the hemolytic toxin aerolysin has been cloned into the plasmid vectors pBR322 and pEMBL8+. The gene was localized on the hybrid plasmids by analysis of plasmids generated by transposon mutagenesis. The sequence of the first 683 bases of an insert in pEMBL8+ was determined and shown to encode the amino terminus of the protein as well as a typical signal sequence of 23 amino acids. Aerolysin is produced by E. coli cells containing the cloned aerolysin gene and it is processed normally by removal of the signal sequence, however it is not released from the cell. The protein appears to be translocated across the inner membrane of E. coli as its signal sequence is removed and the processed protein can be released by osmotic shock.

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Year: 1986 PMID： 3020368 DOI： 10.1007/bf00425512

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

33 in total

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Authors: M Mock; M Schwartz
Journal: J Bacteriol Date: 1978-11 Impact factor: 3.490

2. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

Authors: G N Godson; D Vapnek
Journal: Biochim Biophys Acta Date: 1973-04-11

3. Molecular cloning of the hemolysin determinant from Vibrio cholerae El Tor.

Authors: S L Goldberg; J R Murphy
Journal: J Bacteriol Date: 1984-10 Impact factor: 3.490

Review 4. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.

Authors: F J de Bruijn; J R Lupski
Journal: Gene Date: 1984-02 Impact factor: 3.688

5. Synthesis and secretion of hemolysin by Escherichia coli.

Authors: W Springer; W Goebel
Journal: J Bacteriol Date: 1980-10 Impact factor: 3.490

6. Escherichia coli hemolysin is released extracellularly without cleavage of a signal peptide.

Authors: T Felmlee; S Pellett; E Y Lee; R A Welch
Journal: J Bacteriol Date: 1985-07 Impact factor: 3.490

7. The expression of biologically active cholera toxin in Escherichia coli.

Authors: M L Gennaro; P J Greenaway; D A Broadbent
Journal: Nucleic Acids Res Date: 1982-08-25 Impact factor: 16.971

8. Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli.

Authors: G P Vlasuk; S Inouye; H Ito; K Itakura; M Inouye
Journal: J Biol Chem Date: 1983-06-10 Impact factor: 5.157

Review 4. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas.

Authors: J M Janda
Journal: Clin Microbiol Rev Date: 1991-10 Impact factor: 26.132

8. Relatedness of a periplasmic, broad-specificity RNase from Aeromonas hydrophila to RNase I of Escherichia coli and to a family of eukaryotic RNases.

Authors: D Favre; P K Ngai; K N Timmis
Journal: J Bacteriol Date: 1993-06 Impact factor: 3.490

9. Endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of Vibrio cholerae.

Authors: T D Connell; D J Metzger; J Lynch; J P Folster
Journal: J Bacteriol Date: 1998-11 Impact factor: 3.490

10. Isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in Aeromonas hydrophila.

Authors: S P Howard; J Critch; A Bedi
Journal: J Bacteriol Date: 1993-10 Impact factor: 3.490

Molecular cloning and expression in Escherichia coli of the structural gene for the hemolytic toxin aerolysin from Aeromonas hydrophila.

1. Mechanism of colicin E3 production in strains harboring wild-type or mutant plasmids.

2. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

3. Molecular cloning of the hemolysin determinant from Vibrio cholerae El Tor.

Review 4. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.

5. Synthesis and secretion of hemolysin by Escherichia coli.

6. Escherichia coli hemolysin is released extracellularly without cleavage of a signal peptide.

7. The expression of biologically active cholera toxin in Escherichia coli.

8. Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli.

9. Cellular location of heat-labile enterotoxin in Escherichia coli.

10. Mechanism of protein excretion by gram-negative bacteria: Pseudomonas aeruginosa exotoxin A.

1. Aerolysin, a hemolysin from Aeromonas hydrophila, forms voltage-gated channels in planar lipid bilayers.

2. Hyperproduction, purification, and mechanism of action of the cytotoxic enterotoxin produced by Aeromonas hydrophila.

3. Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.

Review 4. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas.

5. An RNA hairpin sequesters the ribosome binding site of the homing endonuclease mobE gene.

6. Mutagenesis and isolation of Aeromonas hydrophila genes which are required for extracellular secretion.

7. Extracellular secretion of cloned aerolysin and phospholipase by Aeromonas salmonicida.

8. Relatedness of a periplasmic, broad-specificity RNase from Aeromonas hydrophila to RNase I of Escherichia coli and to a family of eukaryotic RNases.

9. Endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of Vibrio cholerae.

10. Isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in Aeromonas hydrophila.