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Literature DB >> 6363391

Cellular location of heat-labile enterotoxin in Escherichia coli.

Abstract

We demonstrated that both the A and B subunits of heat-labile enterotoxin from Escherichia coli are located in the periplasm. The toxin was shown to form aggregates in Tris-EDTA buffers which are routinely used for isolating membranes. The aggregates pellet upon centrifugation, and this may explain why several previous investigators have concluded that enterotoxin is associated with membranes.

Entities: Chemical

Mesh：

Substances：

Year: 1984 PMID： 6363391 PMCID： PMC215294 DOI： 10.1128/jb.157.2.637-642.1984

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

34 in total

1. Cistrons encoding Escherichia coli heat-labile toxin.

Authors: W S Dallas; D M Gill; S Falkow
Journal: J Bacteriol Date: 1979-09 Impact factor: 3.490

2. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors: M J Osborn; J E Gander; E Parisi; J Carson
Journal: J Biol Chem Date: 1972-06-25 Impact factor: 5.157

3. The molecular nature of heat-labile enterotoxin (LT) of escherichia coli.

Authors: W S Dallas; S Falkow
Journal: Nature Date: 1979-02-01 Impact factor: 49.962

4. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli.

Authors: B Witholt; M Boekhout; M Brock; J Kingma; H V Heerikhuizen; L D Leij
Journal: Anal Biochem Date: 1976-07 Impact factor: 3.365

5. Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea.

Authors: H W Smith; M A Linggood
Journal: J Med Microbiol Date: 1971-11 Impact factor: 2.472

6. Membrane-bound enterotoxin of Vibrio cholerae.

Authors: P B Fernandes; M E Bayer
Journal: J Gen Microbiol Date: 1977-12

7. Lincomycin increases synthetic rate and periplasmic pool size for cholera toxin.

Authors: M H Levner; C Urbano; B A Rubin
Journal: J Bacteriol Date: 1980-07 Impact factor: 3.490

8. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli.

Authors: D C Birdsell; E H Cota-Robles
Journal: J Bacteriol Date: 1967-01 Impact factor: 3.490

9. Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli.

Authors: D J Evans; D G Evans; S L Gorbach
Journal: Infect Immun Date: 1974-11 Impact factor: 3.441

10. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin.

Authors: W S Dallas; S Falkow
Journal: Nature Date: 1980-12-04 Impact factor: 49.962

30 in total

1. Purification and characterization of a UDP-glucosyltransferase produced by Legionella pneumophila.

Authors: Iouri Belyi; Michel R Popoff; Nicholas P Cianciotto
Journal: Infect Immun Date: 2003-01 Impact factor: 3.441

Review 2. The mechanism of secretion of hemolysin and other polypeptides from gram-negative bacteria.

Authors: I B Holland; M A Blight; B Kenny
Journal: J Bioenerg Biomembr Date: 1990-06 Impact factor: 2.945

3. A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity.

Authors: A T Aman; S Fraser; E A Merritt; C Rodigherio; M Kenny; M Ahn; W G Hol; N A Williams; W I Lencer; T R Hirst
Journal: Proc Natl Acad Sci U S A Date: 2001-07-10 Impact factor: 11.205

4. Purification and characterization of a low-molecular-weight membrane protein with affinity for the Escherichia coli origin of replication.

Authors: A Jacq; R Kern; A Tsugita; M Kohiyama
Journal: J Bacteriol Date: 1989-03 Impact factor: 3.490

5. Development of an immunoassay using recombinant maltose-binding protein-STa fusions for quantitating antibody responses against STa, the heat-stable enterotoxin of Escherichia coli.

Authors: R Aitken; T R Hirst
Journal: J Clin Microbiol Date: 1992-03 Impact factor: 5.948

6. Coordinated assembly of multisubunit proteins: oligomerization of bacterial enterotoxins in vivo and in vitro.

Authors: S J Hardy; J Holmgren; S Johansson; J Sanchez; T R Hirst
Journal: Proc Natl Acad Sci U S A Date: 1988-10 Impact factor: 11.205

7. Characterization of Erwinia chrysanthemi extracellular proteases: cloning and expression of the protease genes in Escherichia coli.

Authors: C Wandersman; P Delepelaire; S Letoffe; M Schwartz
Journal: J Bacteriol Date: 1987-11 Impact factor: 3.490

8. Protein export by a gram-negative bacterium: production of aerolysin by Aeromonas hydrophila.

Authors: S P Howard; J T Buckley
Journal: J Bacteriol Date: 1985-03 Impact factor: 3.490

9. Extracellular secretion of cloned aerolysin and phospholipase by Aeromonas salmonicida.

Authors: K R Wong; M J Green; J T Buckley
Journal: J Bacteriol Date: 1989-05 Impact factor: 3.490

10. Characterization of the zinc-containing metalloprotease encoded by zpx and development of a species-specific detection method for Enterobacter sakazakii.

Authors: M H Kothary; B A McCardell; C D Frazar; D Deer; B D Tall
Journal: Appl Environ Microbiol Date: 2007-05-04 Impact factor: 4.792