Literature DB >> 3018293

A recombinant murine retrovirus for simian virus 40 large T cDNA transforms mouse fibroblasts to anchorage-independent growth.

M Brown, M McCormack, K G Zinn, M P Farrell, I Bikel, D M Livingston.   

Abstract

A recombinant murine retrovirus containing the intact cDNA sequence for the simian virus 40 (SV40) large T antigen (T) was constructed by using the pZIPNeo SV(X)1 vector. Psi 2 packaging cells were then transfected, and G418-resistant clones were used to generate helper-free viral stocks. NIH 3T3 mouse fibroblasts infected by the recombinant T cDNA retrovirus were selected fro G418 resistance. Such cultures synthesized authentic SV40 T and were transformed to anchorage-independent growth at high efficiency. Therefore, this vector has allowed the study of the transformation properties of T under conditions of neutral drug selection and in the absence of SV40 small t antigen.

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Year:  1986        PMID： 3018293      PMCID： PMC253928     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  22 in total

1.  Comparisons of two early gene functions essential for transformation in polyoma virus and SV-40.

Authors:  M M Fluck; T L Benjamin
Journal:  Virology       Date:  1979-07-15       Impact factor: 3.616

2.  New region of the simian virus 40 genome required for efficient viral transformation.

Authors:  N Bouck; N Beales; T Shenk; P Berg; G di Mayorca
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

3.  Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells.

Authors:  M J Sleigh; W C Topp; R Hanich; J F Sambrook
Journal:  Cell       Date:  1978-05       Impact factor: 41.582

4.  SV40-transformed simian cells support the replication of early SV40 mutants.

Authors:  Y Gluzman
Journal:  Cell       Date:  1981-01       Impact factor: 41.582

5.  Requirement for the large T and small T proteins of SV40 in the maintenance of the transformed state.

Authors:  R J Frisque; D B Rifkin; W C Topp
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1980

6.  Construction and analysis of viable deletion mutants of simian virus 40.

Authors:  T E Shenk; J Carbon; P Berg
Journal:  J Virol       Date:  1976-05       Impact factor: 5.103

7.  Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens.

Authors:  P S Jat; C L Cepko; R C Mulligan; P A Sharp
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272

8.  The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells.

Authors:  R G Martin; V P Setlow; C A Edwards; D Vembu
Journal:  Cell       Date:  1979-07       Impact factor: 41.582

9.  Complex of simian virus 40 large-T antigen and host 53,000-molecular-weight protein in monkey cells.

Authors:  E Harlow; D C Pim; L V Crawford
Journal:  J Virol       Date:  1981-02       Impact factor: 5.103

10.  Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells.

Authors:  F McCormick; E Harlow
Journal:  J Virol       Date:  1980-04       Impact factor: 5.103
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  42 in total

1.  Src family kinases are required for integrin but not PDGFR signal transduction.

Authors:  R A Klinghoffer; C Sachsenmaier; J A Cooper; P Soriano
Journal:  EMBO J       Date:  1999-05-04       Impact factor: 11.598

2.  Simian virus 40 large T antigen targets the spindle assembly checkpoint protein Bub1.

Authors:  Marina Cotsiki; Rowena L Lock; Yuan Cheng; Grace L Williams; Jean Zhao; David Perera; Raimundo Freire; Alan Entwistle; Erica A Golemis; Thomas M Roberts; Parmjit S Jat; Ole V Gjoerup
Journal:  Proc Natl Acad Sci U S A       Date:  2004-01-19       Impact factor: 11.205

3.  Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved.

Authors:  Miao-Hsueh Chen; Christopher W Wilson; Ya-Jun Li; Kelvin King Lo Law; Chi-Sheng Lu; Rhodora Gacayan; Xiaoyun Zhang; Chi-chung Hui; Pao-Tien Chuang
Journal:  Genes Dev       Date:  2009-08-15       Impact factor: 11.361

4.  Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.

Authors:  T L Sladek; J W Jacobberger
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

5.  Rat islet cell lines produced by retroviral transduction of SV40 T antigen.

Authors:  H C Kaung; C Wang; S Xu; J W Jacobberger; W Chen
Journal:  In Vitro Cell Dev Biol Anim       Date:  1996-04       Impact factor: 2.416

6.  Cellular aging is a critical determinant of primary cell resistance to v-src transformation.

Authors:  N Tavoloni; H Inoue
Journal:  J Virol       Date:  1997-01       Impact factor: 5.103

7.  Profile of Myles Brown.

Authors:  Beth Azar
Journal:  Proc Natl Acad Sci U S A       Date:  2018-07-23       Impact factor: 11.205

8.  p38 MAPK enhances STAT1-dependent transcription independently of Ser-727 phosphorylation.

Authors:  Katrin Ramsauer; Iwona Sadzak; Almudena Porras; Andreas Pilz; Angel R Nebreda; Thomas Decker; Pavel Kovarik
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-13       Impact factor: 11.205

9.  Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen.

Authors:  K H Scheidtmann; M C Mumby; K Rundell; G Walter
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

10.  Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development.

Authors:  Jeffrey D Hildebrand; Philippe Soriano
Journal:  Mol Cell Biol       Date:  2002-08       Impact factor: 4.272
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