Literature DB >> 2159569

Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.

T L Sladek1, J W Jacobberger.   

Abstract

Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.

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Year:  1990        PMID： 2159569      PMCID： PMC249510          DOI： 10.1128/JVI.64.6.3135-3138.1990

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  19 in total

1.  Cellular functions are required for the synthesis and integration of avian sarcoma virus-specific DNA.

Authors:  H E Varmus; T Padgett; S Heasley; G Simon; J M Bishop
Journal:  Cell       Date:  1977-06       Impact factor: 41.582

2.  Analysis of intracellular antigens by flow cytometry.

Authors:  J W Jacobberger; D Fogleman; J M Lehman
Journal:  Cytometry       Date:  1986-07

3.  Recombinant retroviruses encoding cell surface antigens as selectable markers.

Authors:  R K Strair; M J Towle; B R Smith
Journal:  J Virol       Date:  1988-12       Impact factor: 5.103

4.  Factors involved in production of helper virus-free retrovirus vectors.

Authors:  A D Miller; D R Trauber; C Buttimore
Journal:  Somat Cell Mol Genet       Date:  1986-03

5.  Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.

Authors:  K Toyoshima; P K Vogt
Journal:  Virology       Date:  1969-07       Impact factor: 3.616

6.  Monoclonal antibodies specific for simian virus 40 tumor antigens.

Authors:  E Harlow; L V Crawford; D C Pim; N M Williamson
Journal:  J Virol       Date:  1981-09       Impact factor: 5.103

7.  Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

Authors:  R Mann; R C Mulligan; D Baltimore
Journal:  Cell       Date:  1983-05       Impact factor: 41.582

8.  Construction and applications of a highly transmissible murine retrovirus shuttle vector.

Authors:  C L Cepko; B E Roberts; R C Mulligan
Journal:  Cell       Date:  1984-07       Impact factor: 41.582

9.  Adsorption of Rous sarcoma virus to genetically susceptible and resistant chicken cells studied by laser flow cytometry.

Authors:  M F Notter; J F Leary; P C Balduzzi
Journal:  J Virol       Date:  1982-03       Impact factor: 5.103

10.  Uniform nomenclature for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus.

Authors:  L Crawford; E Harlow
Journal:  J Virol       Date:  1982-02       Impact factor: 5.103
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  9 in total

1.  Mechanism of entry of a xenotropic MMuLV-derived recombinant retrovirus into porcine cells using the expression of the reporter nlslacZ gene.

Authors:  M A Gilbert; B Charreau; P Vicart; D Paulin; P K Nandi
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

2.  Overexpression of the E2F-1 transcription factor gene mediates cell transformation.

Authors:  X H Yang; T L Sladek
Journal:  Gene Expr       Date:  1995

3.  Growth of anchorage-dependent mammalian cells on plastic petri dishes.

Authors:  X H Yang; T L Sladek
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-06       Impact factor: 2.416

4.  A subset of cells expressing SV40 large T antigen contain elevated p53 levels and have an altered cell cycle phenotype.

Authors:  T L Sladek; J Laffin; J M Lehman; J W Jacobberger
Journal:  Cell Prolif       Date:  2000-04       Impact factor: 6.831

5.  Retrovirus-mediated gene transfer to cystic fibrosis airway epithelial cells: effect of selectable marker sequences on long-term expression.

Authors:  J C Olsen; L G Johnson; M L Wong-Sun; K L Moore; R Swanstrom; R C Boucher
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

6.  E2F-1 has dual roles depending on the cell cycle.

Authors:  Fikret Sahin; Todd L Sladek
Journal:  Int J Biol Sci       Date:  2010-03-03       Impact factor: 6.580

7.  E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity.

Authors:  Fikret Sahin; Todd L Sladek
Journal:  Int J Biol Sci       Date:  2010-07-04       Impact factor: 6.580

8.  Simian virus 40 large T-antigen expression decreases the G1 and increases the G2 + M cell cycle phase durations in exponentially growing cells.

Authors:  T L Sladek; J W Jacobberger
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

9.  Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells.

Authors:  David E Orosz; Philip G Woost; Robert J Kolb; Margaret B Finesilver; Wenwu Jin; Phyllis S Frisa; Chee-Keong Choo; Chung-Fai Yau; Kwok-Wah Chan; Martin I Resnick; Janice G Douglas; John C Edwards; James W Jacobberger; Ulrich Hopfer
Journal:  In Vitro Cell Dev Biol Anim       Date:  2004 Jan-Feb       Impact factor: 2.416
  9 in total

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