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Literature DB >> 3013622

A negative transcriptional control element located upstream of the murine c-myc gene.

Abstract

We have investigated the nature of regulatory sequences within the vicinity of the murine c-myc locus by analyzing the expression of myc-chloramphenicol acetyl transferase (CAT) vectors transfected into a human lymphoblastoid cell line (BJAB) and a monkey fibroblast line (COS). CAT enzymatic assays and S1 nuclease protection experiments reveal that a negative element resides 428-1188 bp 5' of the first c-myc promoter, P1. This 760-bp segment of 5'-flanking c-myc DNA dramatically inhibits CAT gene expression in the pSV2CAT vector when placed in either orientation approximately 1.7 kb 3' (and approximately 3.2 kb 5' on the circular plasmid) from the SV40 promoter region. By employing this strategy, we were unable to identify an analogous DNA segment that is closer to or within the first c-myc exon. We propose that this 5' c-myc region be termed a 'dehancer' since this negative element has the opposite properties of a transcriptional enhancer.

Entities: Chemical

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1986 PMID： 3013622 PMCID： PMC1166880 DOI： 10.1002/j.1460-2075.1986.tb04301.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

40 in total

1. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors: J Casey; N Davidson
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

2. Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with Burkitt and non-Burkitt lymphomas.

Authors: L Zech; U Haglund; K Nilsson; G Klein
Journal: Int J Cancer Date: 1976-01-15 Impact factor: 7.396

3. Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors: B Hirt
Journal: J Mol Biol Date: 1967-06-14 Impact factor: 5.469

4. Short and long range activation by the SV40 enhancer.

Authors: B Wasylyk; C Wasylyk; P Chambon
Journal: Nucleic Acids Res Date: 1984-07-25 Impact factor: 16.971

5. Activation and somatic mutation of the translocated c-myc gene in burkitt lymphoma cells.

Authors: R Taub; C Moulding; J Battey; W Murphy; T Vasicek; G M Lenoir; P Leder
Journal: Cell Date: 1984-02 Impact factor: 41.582

6. A variant translocation places the lambda immunoglobulin genes 3' to the c-myc oncogene in Burkitt's lymphoma.

Authors: G F Hollis; K F Mitchell; J Battey; H Potter; R Taub; G M Lenoir; P Leder
Journal: Nature Date: 1984 Feb 23-29 Impact factor: 49.962

7. Nucleotide sequence comparison of normal and translocated murine c-myc genes.

Authors: L W Stanton; P D Fahrlander; P M Tesser; K B Marcu
Journal: Nature Date: 1984 Aug 2-8 Impact factor: 49.962

8. Regulation of mating-type information in yeast. Negative control requiring sequences both 5' and 3' to the regulated region.

Authors: J Abraham; K A Nasmyth; J N Strathern; A J Klar; J B Hicks
Journal: J Mol Biol Date: 1984-07-05 Impact factor: 5.469

9. Murine T lymphomas in which the cellular myc oncogene has been activated by retroviral insertion.

Authors: L M Corcoran; J M Adams; A R Dunn; S Cory
Journal: Cell Date: 1984-05 Impact factor: 41.582

10. Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma.

Authors: U Siebenlist; L Hennighausen; J Battey; P Leder
Journal: Cell Date: 1984-06 Impact factor: 41.582

48 in total

1. Functional analysis of c-Myb protein in T-lymphocytic cell lines shows that it trans-activates the c-myc promoter.

Authors: J L Evans; T L Moore; W M Kuehl; T Bender; J P Ting
Journal: Mol Cell Biol Date: 1990-11 Impact factor: 4.272

2. Negative regulation of the BZLF1 promoter of Epstein-Barr virus.

Authors: E A Montalvo; Y Shi; T E Shenk; A J Levine
Journal: J Virol Date: 1991-07 Impact factor: 5.103

3. Common factor 1 is a transcriptional activator which binds in the c-myc promoter, the skeletal alpha-actin promoter, and the immunoglobulin heavy-chain enhancer.

Authors: K J Riggs; K T Merrell; G Wilson; K Calame
Journal: Mol Cell Biol Date: 1991-03 Impact factor: 4.272

A negative transcriptional control element located upstream of the murine c-myc gene.

1. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

2. Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with Burkitt and non-Burkitt lymphomas.

3. Selective extraction of polyoma DNA from infected mouse cell cultures.

4. Short and long range activation by the SV40 enhancer.

5. Activation and somatic mutation of the translocated c-myc gene in burkitt lymphoma cells.

6. A variant translocation places the lambda immunoglobulin genes 3' to the c-myc oncogene in Burkitt's lymphoma.

7. Nucleotide sequence comparison of normal and translocated murine c-myc genes.

8. Regulation of mating-type information in yeast. Negative control requiring sequences both 5' and 3' to the regulated region.

9. Murine T lymphomas in which the cellular myc oncogene has been activated by retroviral insertion.

10. Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma.

1. Functional analysis of c-Myb protein in T-lymphocytic cell lines shows that it trans-activates the c-myc promoter.

2. Negative regulation of the BZLF1 promoter of Epstein-Barr virus.

3. Common factor 1 is a transcriptional activator which binds in the c-myc promoter, the skeletal alpha-actin promoter, and the immunoglobulin heavy-chain enhancer.

4. Tissue-specific transcription of the cardiac myosin light-chain 2 gene is regulated by an upstream repressor element.

5. A negative regulatory element with properties similar to those of enhancers is contained within an Alu sequence.

6. Binding of nuclear factor EF-C to a functional domain of the hepatitis B virus enhancer region.

7. Effect of silencer on polyomavirus DNA replication.

8. Negative transcriptional regulatory element that functions in embryonal carcinoma cells.

9. Novel regulation of transcription initiation of the peptide IX gene of adenovirus 2.

10. Rearrangement of rat immunoglobulin E heavy-chain and c-myc genes in the B-cell immunocytoma IR162.