Literature DB >> 2351560

Monoclonal antibodies to human germ cell tumors from "routine" paraffin-embedded pathological specimens.

A Takenaka1, S Kitazawa, S Maeda, S Kamidono.   

Abstract

We have established a method for monoclonal antibody (MoAb) preparation from routine paraffin-embedded tissue of human seminoma as an immunogen. Three 40-microns thick sections were deparaffinized and rehydrated. An eight-week-old BALB/c mouse was immunized intraperitoneally with this extract, which showed no detectable protein bands on sodium laurylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Five monoclonal antibodies (MoAbs) with different characteristics were obtained; one reacted with the nucleus, two with the cytoplasm, and two with the cytoplasmic membrane. One of the MoAbs 5G9 reacted with spermatogonia in normal human tissues and with seminoma, embryonal carcinoma and choriocarcinoma in the testicular tumors.

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Year:  1990        PMID： 2351560     DOI： 10.1007/bf00266786

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  11 in total

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Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

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Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Purification of DNA from formaldehyde fixed and paraffin embedded human tissue.

Authors:  S E Goelz; S R Hamilton; B Vogelstein
Journal:  Biochem Biophys Res Commun       Date:  1985-07-16       Impact factor: 3.575

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Authors:  L Dubeau; L A Chandler; J R Gralow; P W Nichols; P A Jones
Journal:  Cancer Res       Date:  1986-06       Impact factor: 12.701

5.  Purification and analysis of RNA from paraffin-embedded tissues.

Authors:  G M Rupp; J Locker
Journal:  Biotechniques       Date:  1988-01       Impact factor: 1.993

6.  Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.

Authors:  M J Vilela; E P Parrish; D H Wright; D R Garrod
Journal:  J Pathol       Date:  1987-12       Impact factor: 7.996

7.  A monoclonal antibody to human transitional-cell carcinoma of the bladder cross-reacting with a differentiation antigen of neutrophilic lineage.

Authors:  O R Baricordi; A Sensi; C De Vinci; L Melchiorri; G Fabris; E Marchetti; F Corrado; P L Mattiuz; G Pizza
Journal:  Int J Cancer       Date:  1985-06-15       Impact factor: 7.396

8.  Production of a monoclonal antibody specific for seminomas and dysgerminomas.

Authors:  D Bailey; R Baumal; J Law; K Sheldon; P Kannampuzha; M Stratis; H Kahn; A Marks
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

9.  The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  Am J Clin Pathol       Date:  1981-06       Impact factor: 2.493

10.  On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions.

Authors:  H Puchtler; S N Meloan
Journal:  Histochemistry       Date:  1985
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  1 in total

1.  Protruding disordered loop of gC1qR is specifically exposed and related to antiapoptotic property in germ cell lineage.

Authors:  Sohei Kitazawa; Atsushi Takenaka; Takeshi Kondo; Akira Mizoguchi; Riko Kitazawa
Journal:  Histochem Cell Biol       Date:  2006-07-27       Impact factor: 4.304
  1 in total

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