Literature DB >> 3008098

Molecular cloning and sequence analysis of human Na,K-ATPase beta-subunit.

K Kawakami, H Nojima, T Ohta, K Nagano.   

Abstract

We have isolated a cDNA clone for the beta-subunit of HeLa cell Na,K-ATPase, containing a 2208-base-pair cDNA insert covering the whole coding region of the beta-subunit. Nucleotide sequence analysis revealed that the amino acid sequence of human Na,K-ATPase exhibited 61% homology with that of Torpedo counterpart (Noguchi et al. (1986) FEBS Lett. in press). A remarkable conservation in the nucleotide sequence of the 3' non-coding region was detected between the human and Torpedo cDNAs. RNA blot hybridization analysis revealed the presence of two mRNA species in HeLa cells. S1 nuclease mapping indicated that they were derived from utilization of two distinct polyadenylation signals in vivo. Total genomic Southern hybridization indicated the existence of only a few, possibly one set of gene encoding the Na,K-ATPase beta-subunit in the human genome.

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Year:  1986        PMID： 3008098      PMCID： PMC339706          DOI： 10.1093/nar/14.7.2833

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

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Authors:  P S Thomas
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5.  3' non-coding region sequences in eukaryotic messenger RNA.

Authors:  N J Proudfoot; G G Brownlee
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6.  Turnover and regulation of Na-K-ATPase in HeLa cells.

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8.  DNA sequencing with chain-terminating inhibitors.

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  25 in total

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