Literature DB >> 3007941

Plasmid pAO1 of Arthrobacter oxidans encodes 6-hydroxy-D-nicotine oxidase: cloning and expression of the gene in Escherichia coli.

R Brandsch, W Faller, K Schneider.   

Abstract

The 160 kb plasmid pAO1 from Arthrobacter oxidans (Brandsch and Decker 1984) was subcloned in Escherichia coli with the aid of the plasmid vectors pUR222 and pBR322. Screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-D-nicotine oxidase (6-HDNO), one of the enzymes of the nicotine-degradative pathway in A. oxidans, is encoded on pAO1. Immunoprecipitation of 35S-methionine-labelled E. coli cells with 6-HDNO-specific antiserum and expression of recombinant plasmid DNA in E. coli "maxicells" revealed that 6-HDNO is made as a 52,000 dalton protein, approximately 4,500 daltons larger than 6-HDNO from A. oxidans. The 6-HDNO activity was constitutively expressed in E. coli cells, possibly from an A. oxidans promoter, as shown by subcloning of the 6-HDNO gene in pBR322, using the expression vector pKK223-3 and the promoter probe vector pCB192.

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Year:  1986        PMID： 3007941     DOI： 10.1007/bf00330523

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  24 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  [Mechanism of induction of nicotine degrading enzymes in Arthrobacter oxydans].

Authors:  M Gloger; K Decker
Journal:  Z Naturforsch B       Date:  1969-08       Impact factor: 1.047

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.

Authors:  K Decker; H Bleeg
Journal:  Biochim Biophys Acta       Date:  1965-08-24

5.  pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

Authors:  U Rüther
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

6.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

7.  Covalently bound FAD in d-6-hydroxynicotine oxidase. Immunological studies of D- and L-6-hydroxynicotine oxidase: evidence for a D-enzyme precursor.

Authors:  M Brühmüller; A Schimz; L Messmer; K Decker
Journal:  J Biol Chem       Date:  1975-10-10       Impact factor: 5.157

8.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

Authors:  E Amann; J Brosius; M Ptashne
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

10.  Involvement of plasmids in total degradation of chlorinated biphenyls.

Authors:  K Furukawa; A M Chakrabarty
Journal:  Appl Environ Microbiol       Date:  1982-09       Impact factor: 4.792
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  12 in total

Review 1.  Evolution of catabolic pathways: Genomic insights into microbial s-triazine metabolism.

Authors:  N Shapir; E F Mongodin; M J Sadowsky; S C Daugherty; K E Nelson; L P Wackett
Journal:  J Bacteriol       Date:  2006-11-17       Impact factor: 3.490

2.  Degradation of substituted phenylurea herbicides by Arthrobacter globiformis strain D47 and characterization of a plasmid-associated hydrolase gene, puhA.

Authors:  G A Turnbull; M Ousley; A Walker; E Shaw; J A Morgan
Journal:  Appl Environ Microbiol       Date:  2001-05       Impact factor: 4.792

3.  Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species.

Authors:  Cristinel Sandu; Calin-Bogdan Chiribau; Paula Sachelaru; Roderich Brandsch
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

4.  Structural characteristics of the Corynebacterium lilium bacteriophage CL31.

Authors:  A Trautwetter; C Blanco; A M Sicard
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

5.  Cloning and expression in Escherichia coli of a Klebsiella ozaenae plasmid-borne gene encoding a nitrilase specific for the herbicide bromoxynil.

Authors:  D M Stalker; K E McBride
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

6.  Functional analysis of the 5' regulatory region and the UUG translation initiation codon of the Arthrobacter oxidans 6-hydroxy-D-nicotine oxidase gene.

Authors:  L Mauch; V Bichler; R Brandsch
Journal:  Mol Gen Genet       Date:  1990-05

7.  A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16.

Authors:  Hongzhi Tang; Shuning Wang; Lanying Ma; Xiangzhou Meng; Zixin Deng; Dake Zhang; Cuiqing Ma; Ping Xu
Journal:  Appl Environ Microbiol       Date:  2008-01-18       Impact factor: 4.792

8.  Purification and properties of an Arthrobacter oxydans P52 carbamate hydrolase specific for the herbicide phenmedipham and nucleotide sequence of the corresponding gene.

Authors:  H D Pohlenz; W Boidol; I Schüttke; W R Streber
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

9.  Binding of FAD to 6-hydroxy-D-nicotine oxidase apoenzyme prevents degradation of the holoenzyme.

Authors:  R Brandsch; V Bichler; B Krauss
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

10.  Novel nicotine oxidoreductase-encoding gene involved in nicotine degradation by Pseudomonas putida strain S16.

Authors:  Hongzhi Tang; Lijuan Wang; Xiangzhou Meng; Lanying Ma; Shuning Wang; Xiaofei He; Geng Wu; Ping Xu
Journal:  Appl Environ Microbiol       Date:  2008-12-05       Impact factor: 4.792
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