Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The abnormal location of cytoplasmic SV40 large T is not caused by failure to bind to DNA or to p53.

Literature DB >> 3004945

The abnormal location of cytoplasmic SV40 large T is not caused by failure to bind to DNA or to p53.

E Paucha, D Kalderon, W D Richardson, R W Harvey, A E Smith.

Abstract

We have examined the large T encoded by an SV40 mutant, d10, which fails to localize to the nucleus. The DNA sequence of the mutant predicts the alteration of Lys 128----Thr within the sequence 127 Lys Lys Lys Arg Lys 131 of large T. The results show that d10 large T is capable of binding to SV40 DNA, to cellular DNA and to the cellular phosphoprotein p53 as well as wild-type large T. These data suggest that the cytoplasmic location of d10 large T is not due to an inability of the protein to be retained within the nucleus, but argues instead that the protein fails to reach the nucleus because it contains a defective nuclear location signal.

Entities: Chemical

Mesh：

Substances：

Year: 1985 PMID： 3004945 PMCID： PMC554648 DOI： 10.1002/j.1460-2075.1985.tb04071.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

36 in total

1. T antigen is bound to a host protein in SV40-transformed cells.

Authors: D P Lane; L V Crawford
Journal: Nature Date: 1979-03-15 Impact factor: 49.962

2. The binding site on SV40 DNA for a T antigen-related protein.

Authors: R Tjian
Journal: Cell Date: 1978-01 Impact factor: 41.582

3. Extraction and fingerprint analysis of simian virus 40 large and small T-antigens.

Authors: A E Smith; R Smith; E Paucha
Journal: J Virol Date: 1978-10 Impact factor: 5.103

4. Binding of a simian virus 40 T antigen-related protein to DNA.

Authors: R D McKay
Journal: J Mol Biol Date: 1981-01-25 Impact factor: 5.469

5. Differential affinities of simian virus 40 large tumor antigen for DNA.

Authors: M Oren; E Winocour; C Prives
Journal: Proc Natl Acad Sci U S A Date: 1980-01 Impact factor: 11.205

6. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells.

Authors: D I Linzer; A J Levine
Journal: Cell Date: 1979-05 Impact factor: 41.582

7. Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.

Authors: J Davey; N J Dimmock; A Colman
Journal: Cell Date: 1985-03 Impact factor: 41.582

5. Monoclonal antibody specific for human nuclear proteins IEF 8Z30 and 8Z31 accumulates in the nucleus a few hours after cytoplasmic microinjection of cells expressing these proteins.

Authors: P Madsen; S Nielsen; J E Celis
Journal: J Cell Biol Date: 1986-12 Impact factor: 10.539

5 in total

The abnormal location of cytoplasmic SV40 large T is not caused by failure to bind to DNA or to p53.

1. T antigen is bound to a host protein in SV40-transformed cells.

2. The binding site on SV40 DNA for a T antigen-related protein.

3. Extraction and fingerprint analysis of simian virus 40 large and small T-antigens.

4. Binding of a simian virus 40 T antigen-related protein to DNA.

5. Differential affinities of simian virus 40 large tumor antigen for DNA.

6. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells.

7. Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.

8. Simian virus 40 T antigen binds to DNA.

9. Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells.

10. Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.

1. DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae.

2. Signal-dependent translocation of simian virus 40 large-T antigen into rat liver nuclei in a cell-free system.

3. Amino acid sequences that determine the nuclear localization of yeast histone 2B.

4. A nuclear localization signal binding protein in the nucleolus.

5. Monoclonal antibody specific for human nuclear proteins IEF 8Z30 and 8Z31 accumulates in the nucleus a few hours after cytoplasmic microinjection of cells expressing these proteins.