BACKGROUND: Stress-response pathways in Caenorhabditis elegans (C. elegans) were found to be closely related to human diseases and aging. Research on stress responses in C. elegans can therefore significantly facilitate understanding of related human diseases. p-Coumaric acid is present in peanuts, carrots, and garlic, and exerts many biological effects, however, its responses to various environmental stressors remain unknown. Thus, in the current study, we employed C. elegans as the in vivo animal model to examine the function of p-coumaric acid under various stressed conditions. RESULTS: Treatment of C. elegans with p-coumaric acid resulted in a significant reduction in the intercellular reactive oxygen species levels, which suggests the in vivo antioxidant capacity of p-coumaric acid. Moreover, p-coumaric acid significantly increased the worms' survival under oxidative and osmosis stressed conditions but had no effect under normal or heat-stressed conditions. The increased oxidative resistance induced by p-coumaric acid was mediated by skn-1, an ortholog of the Nrf2 (nuclear factor erythroid 2-related factor 2) transcriptional factor. Downregulation of the osmosis regulatory gene, osr-1, might contribute to p-coumaric acids' effect on increased resistance to high osmolarity. CONCLUSION: Taken together, our results suggest that p-coumaric acid, an antioxidant agent, ameliorated oxidative and osmosis stresses in C. elegans.
BACKGROUND: Stress-response pathways in Caenorhabditis elegans (C. elegans) were found to be closely related to human diseases and aging. Research on stress responses in C. elegans can therefore significantly facilitate understanding of related human diseases. p-Coumaric acid is present in peanuts, carrots, and garlic, and exerts many biological effects, however, its responses to various environmental stressors remain unknown. Thus, in the current study, we employed C. elegans as the in vivo animal model to examine the function of p-coumaric acid under various stressed conditions. RESULTS: Treatment of C. elegans with p-coumaric acid resulted in a significant reduction in the intercellular reactive oxygen species levels, which suggests the in vivo antioxidant capacity of p-coumaric acid. Moreover, p-coumaric acid significantly increased the worms' survival under oxidative and osmosis stressed conditions but had no effect under normal or heat-stressed conditions. The increased oxidative resistance induced by p-coumaric acid was mediated by skn-1, an ortholog of the Nrf2 (nuclear factor erythroid 2-related factor 2) transcriptional factor. Downregulation of the osmosis regulatory gene, osr-1, might contribute to p-coumaric acids' effect on increased resistance to high osmolarity. CONCLUSION: Taken together, our results suggest that p-coumaric acid, an antioxidant agent, ameliorated oxidative and osmosis stresses in C. elegans.
Authors: Susiany P Lopes; Lina M Yepes; Yunierkis Pérez-Castillo; Sara M Robledo; Damião P de Sousa Journal: Molecules Date: 2020-07-11 Impact factor: 4.411
Authors: Luciana Fentanes Moura de Melo; Dayanne Lopes Gomes; Lucas Felipe da Silva; Larissa Marina Pereira Silva; Marina Lopes Machado; Cesar Orlando Muñoz Cadavid; Silvana Maria Zucolotto; Riva de Paula Oliveira; Deborah Yara Alves Cursino Dos Santos; Hugo Alexandre Oliveira Rocha; Katia Castanho Scortecci Journal: Oxid Med Cell Longev Date: 2020-10-10 Impact factor: 6.543