| Literature DB >> 30033368 |
Zhihao Zheng1, J Scott Lauritzen1, Eric Perlman1, Camenzind G Robinson1, Matthew Nichols1, Daniel Milkie2, Omar Torrens2, John Price3, Corey B Fisher1, Nadiya Sharifi1, Steven A Calle-Schuler1, Lucia Kmecova1, Iqbal J Ali1, Bill Karsh1, Eric T Trautman1, John A Bogovic1, Philipp Hanslovsky1, Gregory S X E Jefferis4, Michael Kazhdan5, Khaled Khairy1, Stephan Saalfeld1, Richard D Fetter1, Davi D Bock6.
Abstract
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution. To validate the dataset, we traced brain-spanning circuitry involving the mushroom body (MB), which has been extensively studied for its role in learning. All inputs to Kenyon cells (KCs), the intrinsic neurons of the MB, were mapped, revealing a previously unknown cell type, postsynaptic partners of KC dendrites, and unexpected clustering of olfactory projection neurons. These reconstructions show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience. VIDEO ABSTRACT.Entities:
Keywords: Drosophila melanogaster; connectomics; electron microscopy; image stitching; mushroom body; neural circuits; olfaction
Mesh：
Year: 2018 PMID： 30033368 PMCID： PMC6063995 DOI： 10.1016/j.cell.2018.06.019
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582