Total Synthesis of Desmethyl Jahanyne and Its Lipo-Tetrapeptide Conjugates Derived from Parent Skeleton as BCL-2-Mediated Apoptosis-Inducing Agents.

The total synthesis of highly potent and scarcely available marine natural product (-)-jahanyne was attempted resulting in a solution-phase synthesis of pruned versions with comparable activity. A simple and facile synthetic route was employed for the preparation of pruned congeners and would be scalable. The lipophilic tail of the natural product was synthesized from R-(+)-citronellol, utilizing easily available chemicals. All the synthesized compounds were screened for apoptotic activity against a panel of cell lines. These compounds depicted marked binding to B cell lymphoma 2 till 50 °C in cellular thermal shift analysis.

Introduction

Marine cyanobacteria have been an excellent source of new natural products with unique biological profiles.[1,2] Suenaga et al.[3−6] have investigated the constituents of marine cyanobacterium, Lyngbya sp., and isolated an acetylene-containing lipopeptide, jahanyne (1). This marine natural product has a rare lipophilic tail (chiral 2,4-dimethyl decynoic acid 2) and an uncommon hydrophilic head engaging per N-methylated octamer [including Oep (2-(1-oxo-ethyl)-pyrrolidine moiety)]. The N-terminal decynoic acid moiety 2 is reported to be a part structure only once before in carmabin A,[7,8] whose absolute stereochemistry is not reported yet. Jahanyne (1) has shown promising growth inhibition of human cancer cells and induced apoptosis in HeLa cells (1.8 μM). Cell suicide is an important process of all living organisms where unwanted cells are removed from the body. For a healthy being, a balance between cell death and proliferation has to be maintained. The genetic studies on Caenorhabditis elegans and its correlation to human genes have helped to identify a previously unknown gene BCL-2 (B cell lymphoma 2) from the breakpoint region of a recurrent chromosomal translocation in human follicular lymphoma. Enforced overexpression of BCL-2 promoted lymphocyte accumulation in mice studies which resulted either in autoimmune diseases or in cancer.[9,10]

The fascinating biological activity and bolaamphiphile mimicking skeleton feature of jahanyne attracted our attention to take up the total synthesis. Incidentally no total synthesis is reported yet. Herein, we report our attempts toward the total synthesis of jahanyne, where the efforts culminated in the synthesis of desmethyl jahanyne 1a and few pruned analogues 28 and 29. Interestingly, one of these pruned versions had comparable activity with respect to natural product. Disappointingly, the permethylation efforts of converting 1a to 1 were futile. Our efforts also provided lead toward pruning large molecular weight natural products to low-molecular-weight analogues with comparable biological profile.

Retrosynthetically, 1a could be envisioned to be synthesized by coupling of lipophilic acid 2 with the desmethyl octapeptide 3a. This would be a prerequisite advanced scaffold for permethylation. Alternatively, permethylated octapeptide 3 could be directly coupled to 2 to achieve total synthesis of 1. The decynoic acid 2 was envisioned to synthesize from R-(+)-citronellol (which embodies one of the two chiral centers) via Evans’ chemistry to install the second chiral center. Similarly, the desmethyl octapeptide 3a was planned through 5-mer + 3-mer approach by stitching desmethyl pentamer 5 and desmethyl trimer 6. Alternatively, the permethylated octapeptide 3 was planned to be synthesized by coupling permethylated tetramers 7 and 8 (Figure ).

Figure 1

Retrosynthetic analysis.

Results and Discussion

R-(+)-Citronellol (4) was converted to diene 9 following the literature procedure.[11] Hydroboration–oxidation sequence of 9 with 9-BBN and H2O2/NaOH gave alcohol 10 in 85% yield,[12] which on oxidation with Dess–Martin periodinane (DMP) afforded aldehyde 11 in 89% yield.[13] The Corey–Fuchs reaction with CBr4 and n-BuLi followed by the addition of trimethylsilyl chloride yielded the homologated tetramethylsilane (TMS)-alkyne 12 in 72% yield.[14] The isopropylene group in 12 was cleaved using a two-step process,[15,16] epoxidation with meta-chloroperoxybenzoic acid (m-CPBA) followed by the addition of H5IO6 to furnish aldehyde 13, which on oxidation with NaClO2 yielded the acid 14.

The Evans’ auxiliary (XA) was conjugated to acid 14 to furnish imide 15 in 78% yield. Methylation was accomplished using NaHMDS/MeI to provide 16 in 76% yield with 86% de. A column chromatographic separation allowed isolation of pure 16.[17] Exposure of 16 to tetra-n-butylammonium fluoride (TBAF) allowed the cleavage of TMS in acetylenic functionality to generate free alkyne 17 in 93% yield, which upon hydrolysis with LiOH/H2O2 provided the acid unit 2 of jahanyne in 90% yield.[18] The executed route (Scheme ) allowed us to synthesize gram quantities of lipophilic end of jahanyne toward total synthesis.

Scheme 1

Synthesis of Acid Fragment 2

The octapeptide unit, for operational simplicity, was divided into two tetramers 7 and 8. The N-terminal unit 7 was synthesized in a stepwise manner by the addition of desired amino acids in protected form. Thus, the known dimer 18(19) on hydrolysis of ester with lithium hydroxide and coupling with N-Me-l-Val-OMe by following HATU and N,N-diisopropylethylamine (DIPEA) conditions yielded the trimer 19 in 80% over two steps. The hydrolysis of ester 19 with LiOH followed by coupling with N-Me-l-Val-OMe following the same conditions furnished permethyl tetramer 7.

The other permethyl tetramer 8 was synthesized by coupling the known Boc Oep 20(20) with N-Boc-l-Pro following HATU-assisted amidation. The Boc group in 21 was removed with trifluoroacetic acid (TFA); however, the free NH group of proline ring participated in an intramolecular cyclization with keto group of Oep.[4] To circumvent, compound 21 was reduced with NaBH4 to generate diastereomeric alcohol 22. Compound 22 was coupled with N-Boc-N-Me-l-Phe-OH after removing the Boc group in 22 with TFA, oxidation with DMP provided 23 in 61% yield. The resultant 23 on Boc-deprotection followed by coupling with N-Boc-N-Me-l-Val-OH yielded permethyl tetramer 8 in 56% yield (Scheme ).

Scheme 2

Synthesis of Permethyl Tetramers 7 and 8

Reagents and conditions: (a) LiOH, MeOH/H2O; (b) HATU, DIPEA, DMF, 12 h; and (c) TFA, CH2Cl2, 2 h.

With both permethylated tetramers in hand, we tried coupling of them to get the octapeptide 3. N-Boc deprotection of 8 and ester hydrolysis of 7 gave the corresponding amine and acid. However, coupling reagents such as HATU, COMU, PyBrop, and so forth did not yield the required octapeptide 3. In addition, coupling between acid chloride of 7 and free amine of 8 was also unsuccessful. At this point, we revisited our strategy and planned synthesis of desmethyl octapeptide 3a, which on coupling with 2 followed by permethylation would result in 1.

The synthesis of octapeptide 3a was planned from the coupling of pentamer 5 and trimer 3. The dimer 24(21) was subjected to saponification to get free acid, which was coupled with Oep to get trimer 6 in 72% yield (Scheme ). The dimer 25(22) on ester hydrolysis with LiOH followed by coupling with valine methyl ester under EDC, hydroxybenzotriazole (HOBt) conditions gave the trimer 26 in 75% yield. TFA-catalyzed N-Boc hydrolysis of 26 followed by HATU-mediated peptide coupling with dimer 27 resulted in pentamer 5 in 64% yield over two steps. Ester hydrolysis of pentamer 5 with LiOH and coupling with N-Boc deprotected tripeptide 6 resulted in desmethyl octapeptide 3a. Exhaustive permethylation of 3a to 3 was futile under NaH/MeI, Ag2O/MeI,[23] (CH3)2SO4/NaH,[24] dimethyl carbonate/cetrimonium bromide (CTAB),[25] and HCOOMe/CTAB conditions.[26] Condensation of deprotected amine group of 3a with lipophilic acid 2 gave desmethyl jahanyne 1a in 44% yield over two steps.

Scheme 3

Synthesis of Desmethyl Jahanyne

Reagents and conditions: (a) LiOH, MeOH/H2O, 4 h; (b) TFA, CH2Cl2, 2 h; and (c) HATU, DIPEA, DMF, rt, 12 h.

Similarly, our efforts toward the synthesis of 1 from 1a through permethylation were also unsuccessful after several attempts. To explore any of the synthesized intermediates exhibit desired properties, we coupled the peptide units with the acid 2. Thus, we coupled the permethylated tetramer 7 and 8 with 2 individually and isolated the conjugates 28(27) and 29 in 42 and 65% yield, respectively, as pruned versions of the natural product (Scheme ).

Scheme 4

Synthesis of Fatty Acid Conjugates 28 and 29

Reagents and conditions: (a) TFA, CH2Cl2, 2 h and (b) HATU, DMF, DIPEA, rt, 12 h.

At this point, we evaluated all the synthesized compounds against a panel of cell lines to identify compounds with activity in tandem with the reported value of the natural product. The results threw up some interesting observations (Table ). It was observed that tetramer 8 had activity (IC50 1.5 μM) comparable to the value reported in the literature for jahanyne 1 (IC50 1.8 μM) against HeLa cells. Both the conjugates 28 and 29 also exhibited the activity in the range of 3.0 μM on HeLa cells.[3] However, 1a was not effective on the same cell lines.

Table 1

IC50 (μM) Values of Synthetic Analogues of Jahanynea

s. no.	compound no.	MDA-MB-231	DU-145	A549	HeLa	HEK-293
1	18	11 ± 2	8 ± 0.2	28 ± 4	60 ± 16	2453 ± 204
2	19	39 ± 5	3 ± 0.2	6 ± 2	22 ± 3	1208 ± 44
3	7	56 ± 3	3 ± 0.1	3 ± 0.2	23 ± 1	369 ± 25
5	28	3 ± 0.7	1 ± 0.6	1 ± 0.3	3 ± 0.2	97 ± 17
6	17	102 ± 14	188 ± 29	158 ± 15	160 ± 10	293 ± 16
7	2	164 ± 20	123 ± 20	167 ± 12	122 ± 14	259 ± 35
8	21	3 ± 0.1	1 ± 0.9	5 ± 0.4	5 ± 1	5441 ± 245
9	22	8 ± 0.4	2 ± 0.8	0.5 ± 0.03	26 ± 3	3387 ± 123
10	23	6 ± 1	1 ± 0.9	2 ± 0.2	9 ± 1	160 ± 16
11	8	1 ± 0.3	1 ± 0.7	0.2 ± 0.01	1.6 ± 0.1	198 ± 41
12	29	1 ± 0.1	1 ± 0.7	0.2 ± 0.01	3 ± 1	5762 ± 51
14	25	7 ± 0.7	5 ± 1	1 ± 0.3	7 ± 1	238 ± 34
15	26	30 ± 1	17 ± 1	1 ± 0.3	14 ± 1	145 ± 19
16	24	16 ± 2	13 ± 2	6 ± 0.4	12 ± 1	238 ± 34
17	6	55 ± 1	13 ± 2	5 ± 1	6 ± 0.5	273 ± 55
18	5	33 ± 0.4	21 ± 2	1 ± 0.1	4 ± 0.3	412 ± 65
19	3a	9 ± 0.1	1 ± 0.1	2 ± 0.1	12 ± 11	362 ± 33
20	1a	7 ± 0.1	1 ± 0.1	2 ± 0.1	39 ± 1	328 ± 23
21	doxorubicin	1 ± 0.1	0.5 ± 0.2	1 ± 0.1	1 ± 0.2	39 ± 2
22	jahanyneb				1.8

Human breast adenocarcinoma cell line-MDA-MB-231, prostate-DU-145, lung-A549, cervical-HeLa, and primary human embryonic kidney cell line-HEK-293 depicted as mean ± SEM.

Reported literature values. (For raw data, see Supporting Information Table S1).

The most active cytotoxic compounds (compounds 8, 28, and 29) were taken toward identifying their mechanistic roles in cell cycle employing DU-145 cancer cell line.[28] The cell cycle profile depicted compounds (8, 28, and 29) with significant G0/G1 arrest at 1 μM concentration when compared to vehicle control (Figure ). An increased percent of late apoptotic cells was observed when treated with these compounds, similar to positive control, doxorubicin (Figure A). Elevated expression levels of proapoptotic genes including Bax, Bid, and Caspase-3 were found when treated with cytotoxic compounds (8, 28, and 29) for about 24 h at 1 μM concentration as confirmed by the semiquantitative real-time polymerase chain reaction (RT-PCR) techniques (Figure B).[29] The immunoblot assay authenticated that BCL-2 antiapoptotic protein was found at lower levels or absent when treated with compounds 8, 28, and 29 with concomitant increase in the levels of proapoptotic proteins, BAX and Annexin V (Figure C). Thus, the data suggest apoptosis-inducing pathway progresses in these cells by inhibition of BCL-2.

Figure 2

Cell cycle analysis in DU-145 cells treated with compounds 8, 28, and 29 depicted a G0/G1 cell cycle arrest. Doxorubicin was used as a positive control and depicted comparable effect.

Figure 3

Cellular apoptosis in DU-145 cells treated with compounds 8, 28, and 29. (A) Increased percent of cells observed in the late apoptotic phase in the compound-treated group as compared to control. (B) RT-PCR analysis depicted an increased expression of proapoptotic genes Caspase 3, Bax and Bid and concomitant decrease in antiapoptotic gene, Bcl-2. (C) Representative image of immunoblot analysis confirmed the gene expression in these cells at protein expression levels with an increase in proapoptotic proteins BAX, as well as Annexin V.

The navitoclax active site of antiapoptotic BCL-2 protein for molecular docking studies was evaluated to understand the basic interactions with the active compounds (8, 28, and 29) among the series.[30,31] In the case of compound 28, the carbonyl group of proline showed hydrogen bond with bridged water molecule between Arg143 and Asn140; the carbonyl group of valine residue depicted a similar kind of H-bond interaction with Trp105 (Figure A). Both the Oep and proline carbonyl group of compound 8 exhibited two hydrogen bonds (with distances of 1.97 and 2.54 Å) with side chain of Arg104 at a P4 binding pocket of BCL-2 active site (Figure B). Similarly, the terminal carbonyl group of compound 29 had two side-chain hydrogen bonds (with distances of 2.21 and 2.58 Å) with Arg104, and the another terminal portion was found to be accommodated at P2 domain. The aliphatic chain of both compounds 28 and 29 was found to be occupying the P2 binding groove (Figure S1 see Supporting Information).

Figure 4

Molecular docking and CETSA of BCL-2 protein with jahanyne congeners (A) interactions of compound 28 with BCL-2 active site, green sticks indicate compound 28 macromodel sticks point toward amino acids, red color ball and sticks indicate water molecules, and yellow dotted lines indicate hydrogen bonds; (B) surface diagram of compound 8 at P4 binding pocket of BCL-2 active site. (C) CETSA depicting stabilization of BCL-2 in the presence of compounds 8, 29, and 28 at higher temperature (50 °C), indicating binding of these compounds to BCL-2.

To further support the in silico molecular docking of BCL-2, an antiapoptotic protein with compounds 8, 29, and 28 findings, we performed cellular thermal shift assay (CETSA) to elucidate the binding of these compounds to BCL-2 in DU-145 cells. CETSA evaluates the binding of a ligand or a small molecule to its target protein that leads to increased target protein stabilization at differential temperature, as described earlier.[32,33] Optimum BCL-2 stabilization by compounds 8, 29, and/or 28 binding was observed till 46 °C (Figure C). Cells were treated with 8, 29, and 28 and depicted BCL-2 expression at 50 °C as compared to unbound protein in vehicle-treated cells. The significant shift in melting temperatures of BCL-2 and its stabilization upon the addition of 8, 29, and 28 indicated that jahanyne congeners may be binding to this antiapoptotic protein (BCL-2).

In summary, we have synthesized pruned congeners of jahanyne using simple and scalable steps. Mechanistic studies indicated that BCL-2 inhibition could be the reason for apoptotic activity of the molecules. The dismal biological activity of desmethyl jahanyne substantiated the observation that N-methylation plays a critical role in the activity of the molecule. Further applications of BCL-2 inhibition in the therapeutic areas of treatment of autoimmune diseases and parasites such as Schistosoma will be explored.

General Information

Unless otherwise noted, all reagents were used as received from commercial suppliers. All nonaqueous reactions were performed under an atmosphere of nitrogen using an oven-dried glassware and a standard syringe in septa techniques. All solvents were dried before use, following the standard procedures. Reactions were monitored using thin-layer chromatography (TLC) (SiO2). TLC plates were visualized with UV light (254 nm), iodine treatment, or using ninhydrin stain. Column chromatography was carried out using silica gel (100–200 mesh) packed in glass columns. NMR spectra were recorded at 300, 400, 500 MHz (H) and at 75, 101, 126 MHz (C). Chemical shifts (δ) are reported in parts per million, using the residual solvent peak in CDCl3 (H: δ = 7.26 and C: δ = 77.16 ppm) as an internal standard, and coupling constants (J) are given in hertz. High-resolution mass spectrometry (HRMS) was recorded using electrospray ionization (ESI)–Time-of-flight techniques. All N-methyl amino acids were prepared according to the procedure described in the literature.[34−36]

Biological Data

Antiproliferative Activity of Compounds

Sulforhodamine B assay was performed to evaluate the effect of antiproliferative activity of synthesized analogues in various human tissue-specific cancer cell lines breast-MDA-MB-231, prostate-DU-145, lung-A549, cervical-HeLa and control cell line, primary human embryonic kidney cell line-HEK-293. Among these cell lines, DU-145 depicted higher sensitivity toward the synthesized analogues of all the compounds evaluated; compounds 28, 8, and 29 were observed to be effective against all the cancer cell types with IC50 values ranging between 0.15 and 3.15 μM. Doxorubicin, a standard drug, was used as a positive control. These compounds rendered ineffective against HEK-293 (IC50 ≥ 100 μM), suggesting their nontoxic property against normal cells.

Effect of Compounds on Cell Cycle Profile

Cell cycle analysis was performed on DU-145 as described in the methods using PI staining. The results depicted an increase in the G0/G1 arrest in cells treated with compounds 28, 8, and 29 as compared to vehicle control.

Effect of Compounds on Apoptotic Gene Expression

Gene expression studies were performed with a semiquantitative RT-PCR. Briefly, DU-145 cells were treated with compounds at 1 μM concn for 24 h. Proapoptotic genes, Bax and Bid as well as Caspase-3, a programmed cell death marker, were found to be highly expressed in compounds 28, 8, and 29 as compared to vehicle control. BCL-2, an antiapoptotic gene expression, was very low to absent in cells treated with compounds 28, 8, and 29 compounds, suggesting a proapoptotic role of these compounds.

Effect of Compounds on Apoptotic Protein Expression

Antiapoptotic protein marker, BCL-2 expression, was low, whereas proapoptotic marker, BAX and Annexin V, was highly expressed in DU-145 cells treated with compounds 28, 8, and 29 as well as doxorubicin (positive control) when compared with vehicle control. Compound 9 was found to be active against all the cell lines at 1 μM concn except for HEK-293. These results indicate that Oep moiety is a major contributor to the antiapoptotic activity of compounds synthesized.

Experimental Section

(S)-6,10-Dimethylundeca-1,9-diene (9)

To a stirred solution of PPh3 (8 g, 30.0 mmol) and imidazole (4.2 g, 60.0 mmol) in CH2Cl2 (20 mL) was added I2 at 0 °C followed by R-(+) citronellol (3.19 g, 20.4 mmol) in CH2Cl2 (10 mL) and stirred the solution at room temperature (rt) for 1 h. The reaction mixture was quenched with sat. sodium thiosulfate (20 mL) and extracted with CH2Cl2 (100 mL); the organic layer was washed with brine (50 mL), then dried over Na2SO4, and concentrated; and the residue was passed through the celite pad with hexane to afford the iodo compound as a colorless oil (4.9 g). The iodo compound (4.9 g, 18.4 mmol) was dissolved in dry THF (30 mL), and allylmagnesium bromide (1 M, 128 mL) was added at 0 °C and then stirred for 12 h at rt. Sat. NH4Cl was carefully added at 0 °C and then stirred the reaction mixture for 10 min. The mixture was filtered through the celite pad and extracted with Et2O (3 × 20 mL). Organic layer was dried over Na2SO4 and concentrated, and the residue was purified by flash chromatography (hexane) to afford a colorless oil 9 (2.5 g, 70% in two steps); [α]D20 −0.68 (c = 1.0, CHCl3); 1H NMR (500 MHz, CDCl3): δ 5.85–5.77 (m, 1H), 5.13–5.07 (m, 1H), 5.03–4.91 (m, 2H), 2.07–1.89 (m, 4H), 1.68 (s, 3H), 1.60 (s, 3H), 1.46–1.29 (m, 5H), 1.17–1.09 (m, 2H), 0.87 (d, J = 6.6 Hz, 3H); 13C NMR (101 MHz, CDCl3): δ 139.1, 130.8, 125.0, 114.1, 37.1, 36.5, 34.2, 32.3, 26.4, 25.7, 25.6, 19.6, 17.6; IR (neat): νmax 2970, 2839, 2173, 1606, 1511, 1455, 1378, 1250, 773, 760 cm–1; HRMS (EI-MS) calcd for C13H24 [M]+: 180.1878; found, 180.1872.

(S)-6,10-Dimethylundec-9-en-1-ol (10)

A flame-dried flask was cooled under a stream of nitrogen and charged with diene 9 (2.3 g, 12.7 mmol) and dry THF (10 mL). A solution of 9-BBN (25 mL, 0.5 M in THF, 12.7 mmol) was added dropwise, and the resulting mixture was stirred at rt for 12 h. Then, the reaction was cooled to rt, and an aqueous solution of NaOH (3 M, 10 mL) followed by an aqueous solution of H2O2 (30%, 10 mL) were added. Stirring was continued at rt for 6 h. Et2O (50 mL) was added, the layers were separated, and the aqueous layer was extracted with Et2O (3 × 50 mL). The organic layers were combined, dried over Na2SO4, and concentrated under reduced pressure. The crude material was purified by column chromatography (10% EtOAc/hexane) to afford colorless oil 10 (2.12 g, 85%); [α]D20 −1.05 (c = 2.1, CHCl3); 1H NMR (500 MHz, CDCl3): δ 5.10 (t, J = 7.1 Hz, 1H), 3.63 (t, J = 6.7 Hz, 2H), 2.07–1.89 (m, 2H), 1.68 (s, 3H), 1.60 (s, 3H), 1.59–1.52 (m, 2H), 1.44–1.24 (m, 8H), 1.18–1.08 (m, 2H), 0.86 (d, J = 6.6 Hz, 3H); 13C NMR (101 MHz, CDCl3): δ 131.0, 125.0, 63.0, 37.1, 36.9, 32.8, 32.4, 26.8, 26.1, 25.7, 25.6, 19.6, 17.6; IR (neat): νmax 3471, 2957, 2934, 1606, 1456, 1252, 1045, 773 cm–1; HRMS (ESI) calcd for C13H27O [M + H]+: 199.2056; found, 199.2055.

(S)-6,10-Dimethylundec-9-enal (11)

DMP (6.4 g, 15.1 mmol) was added at 0 °C under nitrogen to a solution of alcohol 10 (2.0 g, 10.1 mmol) in CH2Cl2 (20 mL). The resulting mixture was stirred at rt for 1 h. A 1:1 saturated NaHCO3/Na2S2O3 solution (20 mL) was then added; the aqueous phase was separated and extracted with CH2Cl2 (100 mL). The combined organic extracts were dried (Na2SO4), filtered, and concentrated. Purification of the residue by flash chromatography on silica gel (5% EtOAc in hexane) gave aldehyde 11 (1.7 g, 89%) as a colorless oil; [α]D20 −0.28 (c = 3.15, CHCl3); 1H NMR (300 MHz, CDCl3): δ 9.77 (t, J = 1.8 Hz, 1H), 5.24–4.93 (m, 1H), 2.44 (td, J = 7.3, 1.8 Hz, 2H), 2.07–1.90 (m, 2H), 1.69 (s, 3H), 1.61 (s, 4H), 1.43–1.27 (m, 6H), 1.19–1.09 (m, 2H), 0.87 (d, J = 6.4 Hz, 3H); 13C NMR (101 MHz, CDCl3): δ 202.4, 130.8, 124.7, 43.7, 36.9, 36.5, 32.1, 26.4, 25.5, 25.4, 22.2, 19.3, 17.4 cm–1; HRMS (ESI) calcd for C13H25O [M + H]+: 197.1900; found, 197.1902.

(S)-(7,11-Dimethyldodec-10-en-1-yn-1-yl)trimethylsilane (12)

CBr4 (3.31 g, 10.2 mmol) was added slowly to a solution of PPh3 (5.24 g, 20.4 mmol) in CH2Cl2 (20 mL) at 0 °C, and stirring was continued for 30 min. A solution of aldehyde 11 (1.0 g, 5.1 mmol) in CH2Cl2 (5 mL) was added dropwise at the same temperature, and stirring was continued at rt for 2 h. The reaction mixture was concentrated and filtered through a celite pad with Et2O. The solvent was removed in vacuum, and the crude dibromoalkene was dissolved in THF (20 mL). The solution was cooled to −78 °C; a solution of n-BuLi (2.5 M in hexane, 4 mL, 10.2 mmol) was added slowly, and stirring was continued for 1 h. Chlorotrimethylsilane (1.3 mL, 10.2 mmol) was added slowly; the resulting mixture was allowed to warm to rt and stirred for 12 h. The mixture was poured into saturated aqueous NH4Cl (20 mL). The organic layer was separated, and the aqueous phase was extracted with diethyl ether (150 mL). The combined organic layers were dried over Na2SO4 and concentrated in vacuo. Column chromatography on silica gel (hexane) provided the enyne 12 (0.93 g, 72%) as a colorless oil; [α]D20 −1.48 (c = 1.0, CHCl3); 1H NMR (400 MHz, CDCl3): δ 5.09 (t, J = 6.7 Hz, 1H), 2.21 (t, J = 7.0 Hz, 2H), 2.06–1.86 (m, 2H), 1.68 (s, 3H), 1.59 (s, 3H), 1.54–1.26 (m, 7H), 1.19–1.06 (m, 2H), 0.86 (d, J = 6.5 Hz, 3H), 0.14 (s, 9H); 13C NMR (101 MHz, CDCl3): δ 131.0, 125.0, 107.7, 84.3, 37.1, 36.3, 32.2, 28.9, 26.1, 25.7, 25.5, 19.8, 19.5, 17.6, 0.2; IR (neat): νmax 3312, 2954, 1721, 1606, 1315, 1237, 1156, 1052, 956, 852 cm–1; HRMS (EI-MS) calcd for C17H32Si [M]+: 264.2273; found, 264.2270.

4-Methyl-10-(trimethylsilyl)dec-9-ynal (13)

Enyne 12 (500 mg, 1.89 mmol) and sodium acetate (162 mg, 1.98 mmol) were suspended in CH2Cl2 (10 mL) and cooled to −20 °C. m-CPBA (70%, 488 mg, 1.98 mmol) was added in small portions, and stirring was continued for 1.5 h, allowing the suspension to warm to 0 °C. The reaction was quenched by careful addition of saturated aqueous NaHCO3 (10 mL) and extracted with CH2Cl2 (3 × 10 mL). The combined organic fractions were washed with 1 N NaOH (5 mL), dried over Na2SO4, and concentrated in vacuo. The crude product was dissolved in Et2O (10 mL) and cooled to 0 °C, and H5IO6 (647 mg, 2.84 mmol) in THF (15 mL) was added within 45 min. Stirring was continued until TLC analysis showed complete consumption of the starting material. The mixture was diluted with Et2O (30 mL); H2O (10 mL) was added, and the phases were separated. The combined organic layers of residue were washed twice with brine, dried over Na2SO4, and concentrated in vacuo. Column chromatography on silica gel (5% EtOAc in hexane) provided the aldehyde 13 (372 mg, 84%) as a colorless liquid; [α]D20 −1.07 (c = 1.0, CHCl3); 1H NMR (300 MHz, CDCl3): δ 9.76 (t, J = 1.8 Hz, 1H), 2.53–2.27 (m, 2H), 2.21 (t, J = 6.9 Hz, 2H), 1.75–1.58 (m, 1H), 1.55–1.10 (m, 8H), 0.87 (d, J = 6.2 Hz, 3H), 0.13 (s, 9H); 13C NMR (75 MHz, CDCl3): δ 202.8, 107.4, 84.4, 41.6, 36.0, 32.2, 28.8, 28.7, 26.0, 19.8, 19.2, 0.1; IR (neat): νmax 3315, 2942, 1716, 1605, 1532, 1239, 1156, 962, 779 cm–1; HRMS (ESI) calcd for C14H26NaOSi [M + Na]+: 261.1645; found, 261.1655.

(S)-4-Methyl-10-(trimethylsilyl)dec-9-ynoic Acid (14)

The aldehyde 13 (350 mg, 1.47 mmol) was dissolved in t-butanol (12 mL) and H2O (4 mL). 2-Methyl-2-butene (1.24 mL, 117 mmol), sodium dihydrogen phosphate (710 mg, 5.88 mmol), and sodium chlorite (529 mg, 5.88 mmol) were added to the solution, and the mixture was stirred for 1.5 h at rt. The solvent was removed in vacuo; the residue was dissolved in EtOAc (200 mL) and washed with brine solution. The aqueous phase was extracted with EtOAc (3 × 20 mL); the combined organic layers of residue were washed with brine, dried over Na2SO4, and concentrated in vacuo. Column chromatography on silica gel (10% EtOAc in hexane) provided the acid 14 (360 mg, 96%) as a colorless liquid; [α]D20 −0.86 (c = 0.75, CHCl3); 1H NMR (400 MHz, CDCl3): δ 2.42–2.29 (m, 2H), 2.21 (t, J = 7.0 Hz, 2H), 1.76–1.60 (m, 1H), 1.56–1.25 (m, 7H), 1.22–1.10 (m, 1H), 0.88 (d, J = 6.3 Hz, 3H), 0.13 (s, 9H); 13C NMR (101 MHz, CDCl3): δ 180.6, 107.5, 84.4, 35.9, 32.2, 31.8, 31.5, 28.7, 25.9, 19.8, 19.1, 0.1; IR (neat): νmax 3321, 2954, 1758, 1726, 1521, 1043, 952, 843, 768 cm–1; HRMS (ESI) calcd for C14H26NaO2Si [M + Na]+: 277.1594; found, 277.1609.

(R)-4-Benzyl-3-((S)-4-methyl-10-(trimethylsilyl)dec-9-ynoyl)oxazolidin-2-one (15)

To a stirred solution of acid 14 (0.36 g, 1.41 mmol) and Et3N (0.5 mL, 3.54 mmol) in anhydrous THF (10 mL) at −20 °C was added pivaloyl chloride (0.2 mL, 1.55 mmol) dropwise, and the mixture was stirred for 1 h at −20 °C. Anhydrous LiCl (1.01 g, 23.93 mmol) was then added, and the mixture was stirred for 30 min. To this mixture, a solution of auxiliary (275 mg, 1.55 mmol) in anhydrous THF was added; the mixture was stirred at −20 °C for 1 h and then allowed to warm to rt. After stirring at ambient temperature for 2 h, the reaction mixture was quenched with sat. NH4Cl solution (15 mL). The organic layer was separated, and the aqueous layer was extracted with EtOAc (75 mL). The combined organic layers were washed with brine and dried over Na2SO4 and concentrated in vacuo. Column chromatography on silica gel (10% EtOAc in hexane) provided imide 15 (0.456 g, 78%) as a yellow liquid; [α]D20 −33.38 (c = 2.1, CHCl3); 1H NMR (400 MHz, CDCl3): δ 7.38–7.16 (m, 5H), 4.69–4.62 (m, 1H), 4.24–4.09 (m, 2H), 3.27 (dd, J = 13.4, 3.2 Hz, 1H), 2.99–2.88 (m, 2H), 2.75 (dd, J = 13.3, 9.6 Hz, 1H), 2.21 (t, J = 7.0 Hz, 2H), 1.82–1.63 (m, 1H), 1.59–1.13 (m, 8H), 0.91 (d, J = 6.3 Hz, 3H), 0.13 (s, 9H); 13C NMR (75 MHz, CDCl3): δ 173.6, 153.4, 135.3, 129.4, 128.9, 127.3, 107.5, 84.3, 66.1, 55.1, 37.9, 36.1, 33.3, 32.3, 31.2, 28.8, 26.0, 19.8, 19.3, 0.1; IR (neat): νmax 3328, 1788, 1717, 1521, 1142, 1052, 992, 773 cm–1; HRMS (ESI) calcd for C24H35NNaO3Si [M + Na]+: 436.2278; found, 436.2297.

(R)-4-Benzyl-3-((2R,4S)-2,4-dimethyl-10-(trimethylsilyl)dec-9-ynoyl)oxazolidin-2-one (16)

To a stirred solution of 15 (350 mg, 0.847 mmol) in anhydrous THF (8 mL) under nitrogen was added NaHMDS (1 M in THF, 1.0 mL, 1.01 mmol) at −78 °C. After stirring for 30 min, methyl iodide (0.53 mL, 8.47 mmol) was added and the mixture was stirred at −78 °C for another 1 h. The reaction mixture was quenched with saturated aqueous NH4Cl solution (10 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in vacuo. Column chromatography on silica gel (10% EtOAc in hexane) provided 16 (275 mg, 76%) as a yellow liquid; [α]D20 −50.43 (c = 1.75, CHCl3); 1H NMR (500 MHz, CDCl3): δ 7.33 (dd, J = 10.0, 4.5 Hz, 2H), 7.30–7.25 (m, 1H), 7.24–7.19 (m, 2H), 4.71–4.59 (m, 1H), 4.27–4.10 (m, 2H), 3.91–3.75 (m, 1H), 3.27 (dd, J = 13.3, 3.2 Hz, 1H), 2.76 (dd, J = 13.3, 9.6 Hz, 1H), 2.20 (t, J = 7.1 Hz, 2H), 1.57–1.38 (m, 6H), 1.37–1.25 (m, 2H), 1.20 (d, J = 6.8 Hz, 3H), 1.17–1.10 (m, 1H), 0.88 (d, J = 6.4 Hz, 3H), 0.14 (s, 9H); 13C NMR (101 MHz, CDCl3): δ 177.7, 153.0, 135.3, 129.4, 128.9, 127.3, 107.5, 84.4, 66.0, 55.4, 40.4, 37.9, 36.6, 35.4, 30.3, 28.7, 26.0, 19.8, 19.2, 17.2, 0.2; IR (neat): νmax 3328, 1768, 1737, 1551, 1198, 1074, 984, 763 cm–1; HRMS (ESI) calcd for C25H37NNaO3Si [M + Na]+: 450.2435; found, 450.2456.

(R)-4-Benzyl-3-((2R,4S)-2,4-dimethyldec-9-ynoyl)oxazolidin-2-one (17)

To a solution of 16 (0.25 g, 0.58 mmol) in THF (10 mL) at −10 °C was added TBAF (1.0 M/THF, 1.46 mL, 1.46 mmol) over 15 min. The resulting orange solution was stirred for 1 h at this temperature then diluted with EtOAc (50 mL). The residue was washed with sat. NH4Cl and brine and dried over Na2SO4 and concentrated in vacuo. The crude was purified by flash chromatography (10% EtOAc/hexane) to afford 17 (0.193 g, 93%) as a colorless liquid; [α]D20 −49.40 (c = 0.6, CHCl3); 1H NMR (500 MHz, CDCl3): δ 7.33 (dd, J = 10.0, 4.5 Hz, 2H), 7.29–7.26 (m, 1H), 7.24–7.19 (m, 2H), 4.74–4.58 (m, 1H), 4.28–4.10 (m, 2H), 3.89–3.78 (m, 1H), 3.27 (dd, J = 13.4, 3.3 Hz, 1H), 2.76 (dd, J = 13.4, 9.6 Hz, 1H), 2.20–2.16 (m, 2H), 1.93 (t, J = 2.6 Hz, 1H), 1.59–1.23 (m, 9H), 1.20 (d, J = 6.8 Hz, 3H), 0.89 (d, J = 6.4 Hz, 3H); 13C NMR (75 MHz, CDCl3): δ 177.6, 153.0, 135.3, 129.4, 128.9, 127.3, 84.6, 68.1, 66.0, 55.4, 40.4, 37.9, 36.7, 35.4, 30.3, 28.6, 26.0, 19.2, 18.3, 17.1; IR (neat): νmax 3016, 1768, 1732, 1511, 1162, 1095, 992, 762, cm–1; HRMS (ESI) calcd for C22H29NNaO3 [M + Na]+: 378.2040; found, 378.2063.

(2R,4S)-2,4-Dimethyldec-9-ynoic Acid (2)

To a solution of 17 (180 mg, 0.5 mmol) in THF (10 mL) were added hydrogen peroxide (245 mg, 2.5 mmol, 35%) and LiOH·H2O (42 mg, 1.0 mmol) at 0 °C. After 5 min at 0 °C, the reaction mixture was allowed to rt and stirred for 2 h. The reaction mixture was cooled to 0 °C and quenched with sat. Na2SO3 at 0 °C. The residue was diluted with H2O, acidified with 9 M HCl until pH reached to 2.0, and extracted with EtOAc (3 × 10 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in vacuo. Column chromatography on silica gel (10% EtOAc in hexane) provided 2 (90 mg, 90%) as a colorless liquid; [α]D20 −3.61 (c = 1.5, CHCl3); 1H NMR (400 MHz, CDCl3): δ 2.59–2.47 (m, 1H), 2.18 (td, J = 6.9, 2.5 Hz, 2H), 1.94 (t, J = 2.6 Hz, 1H), 1.62–1.44 (m, 4H), 1.43–1.30 (m, 5H), 1.15 (d, J = 6.9 Hz, 3H), 0.87 (d, J = 6.3 Hz, 3H); 13C NMR (101 MHz, CDCl3): δ 183.6, 84.6, 68.2, 40.7, 37.1, 36.4, 30.4, 28.9, 26.0, 19.3, 18.4, 16.8; IR (neat): νmax 3315, 1757, 1561, 1102, 1085, 962, 753 cm–1; HRMS (ESI) calcd for C12H19O2 [M – H]−: 195.1380; found, 195.1391.

N-Me-N-Boc-l-Ala-l-Pro-N-Me-l-Val-OMe (19)

To a stirred solution of dimer 18 (1 g, 3.18 mmol) in MeOH/H2O (7:3, 10 mL) was added LiOH·H2O (400 mg, 9.5 mmol), and the reaction mixture was stirred at rt for 3 h. After that, the solvent (MeOH) was removed in vacuo and the resulting mixture was acidified with 1 M HCl (0.3 mL) to pH 4 and extracted with EtOAc (3 × 10 mL). The combined organic layer was washed with brine, dried over Na2SO4, and concentrated. The crude material was dissolved in dry dimethylformamide (DMF) (5 mL). To this, the trifluoroacetate salt of N-methyl valine ester (0.62 g, 2.51 mmol), dry DMF (3 mL) and DIPEA (1.8 mL, 10.04 mmol) were added at rt. The suspension was stirred for 10 min, and HATU (1.43 g, 3.76 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (50% EtOAc in hexane) to give the trimer 19 (1.08 g, 80%) as a colorless liquid; [α]D20 −174.82 (c = 2.0, CHCl3); mixture of rotamers (9:1), 1H NMR (400 MHz, CDCl3): δ 4.99–4.92 (m, 0.5H), 4.84–4.64 (m, 2.5H), 3.73–3.67 (m, 0.5H), 3.63 (s, 3H), 3.56–3.33 (m, 1.5H), 3.05 (s, 2.7H), 2.89 (s, 0.3H), 2.81 (s, 0.4H), 2.76–2.70 (m, 2.6H), 2.31–2.03 (m, 3H), 1.93–1.78 (m, 2H), 1.39 (s, 9H), 1.24–1.15 (m, 3H), 0.94–0.91 (m, 3H), 0.88–0.85 (m, 3H); 13C NMR (126 MHz, CDCl3): δ 172.8, 171.7, 170.0, 169.2, 155.4, 154.8, 80.0, 79.6, 65.3, 61.5, 61.4, 56.7, 56.5, 53.5, 51.5, 51.5, 46.7, 46.4, 38.4, 31.1, 29.4, 29.0, 28.2, 27.4, 25.6, 24.6, 19.5, 18.9, 18.5, 14.0, 13.6; IR (neat): νmax 2932, 2864, 1751, 1684, 1617, 1481, 1266, 1059, 972, 840 cm–1; HRMS (ESI) calcd for C21H37N3NaO6 [M + Na]+: 450.2575; found, 450.2583.

Tetramer 7

To a stirring solution of trimer 19 (0.5 g, 1.17 mmol) in MeOH/H2O (7:3, 10 mL) was added LiOH·H2O (147 mg, 3.5 mmol), and the reaction mixture was stirred at rt for 3 h. After that, the solvent (MeOH) was removed in vacuo and the resulting mixture was acidified with 1 M HCl (0.3 mL) to pH 4 and extracted with EtOAc (3 × 10 mL). The combined organic layer was washed with brine, dried over Na2SO4, and concentrated. The crude was dissolved in dry DMF (3 mL); to this, the trifluoroacetate salt of N-methyl valine ester (0.219 g, 0.895 mmol), dry DMF (2 mL) and DIPEA (0.62 mL, 3.58 mmol) were added at rt. The suspension was stirred for 10 min, and HATU (0.51 g, 1.34 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (50% EtOAc in hexane) to give tetramer 7 (440 mg, 70%) as a colorless liquid; [α]D20 −125.97 (c = 1.3, CHCl3); mixture of rotamers (7:2), 1H NMR (500 MHz, CDCl3): δ 5.11 (d, J = 10.7 Hz, 0.8H), 5.06–4.99 (m, 0.9H), 4.84 (d, J = 11.1 Hz, 1H), 4.79 (d, J = 13.1 Hz, 1.1H), 4.40 (d, J = 8.9 Hz, 0.2H), 3.84 (dd, J = 7.3, 4.6 Hz, 0.2H), 3.67 (s, 3H), 3.61–3.43 (m, 1.8H), 3.08 (s, 2.5H), 3.02 (s, 2.7H), 2.97 (s, 0.3H), 2.86 (s, 0.5H), 2.76 (s, 2.4H), 2.71 (s, 0.6H), 2.41–2.28 (m, 1.5H), 2.21–2.08 (m, 2.3H), 2.03–1.82 (m, 1.4H), 1.91 (s, 0.8H), 1.43 (s, 7H), 1.39 (s, 2H), 1.24 (d, J = 9.6 Hz, 3H), 1.04–1.01 (m, 1H), 1.00–0.97 (m, 2H), 0.93–0.90 (m, 3H), 0.90–0.87 (m, 3H), 0.87–0.85 (m, 1H), 0.75–0.73 (m, 2H); 13C NMR (75 MHz, CDCl3): δ 172.6, 171.6, 171.2, 170.7, 155.5, 154.9, 80.2, 79.8, 64.3, 61.3, 58.4, 57.9, 57.2, 56.9, 53.7, 51.8, 51.6, 46.8, 46.5, 31.5, 30.2, 30.1, 29.8, 29.5, 29.1, 28.4, 27.6, 27.4, 27.0, 24.7, 20.0, 19.9, 19.1, 18.9, 18.6, 18.3, 14.1; IR (neat): νmax 2973, 2876, 2839, 1740, 1676, 1651, 1535, 1442, 1390, 1150, 1081, 868, 771 cm–1; HRMS (ESI) calcd for C27H48N4NaO7 [M + Na]+: 563.3415; found, 563.3443.

tert-Butyl(S)-2-((S)-2-acetylpyrrolidine-1-carbonyl)pyrrolidine-1-carboxylate (21)

To a stirred solution of Oep 20 (1.0 g, 4.69 mmol) in CH2Cl2 (10 mL) was added TFA (0.68 mL, 13.1 mmol), and the reaction mixture was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (5 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the trifluoroacetate salt of deprotected 20 was afforded and used in the next reaction without purification. To a 50 mL RB flask, trifluoroacetate salt of deprotected 20, dry DMF and dry DIPEA (3.3 mL, 18.7 mmol) were added at rt; to this, acid N-Boc-l-proline (1.21 g, 5.6 mmol) in dry DMF (3 mL) was added. The suspension was stirred for 10 min, and HATU (2.67 g, 7.0 mmol) was added under N2 atmosphere. After stirring for 6 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (50% EtOAc in hexane) to give the 21 (1.16 g, 80%) as a colorless liquid; [α]D20 −84.57 (c = 2.55, CHCl3); mixture of rotamers (2:1), 1H NMR (500 MHz, CDCl3): δ 4.61 (dd, J = 8.8, 4.7 Hz, 0.6H), 4.57 (dd, J = 8.5, 4.5 Hz, 0.4H), 4.43 (dd, J = 8.5, 3.0 Hz, 0.6H), 4.32 (dd, J = 8.4, 4.0 Hz, 0.4H), 3.75–3.68 (m, 0.6H), 3.62–3.56 (m, 0.4H), 3.55–3.45 (m, 2H), 3.42–3.36 (m, 0.4H), 3.34–3.29 (m, 0.6H), 2.14 (s, 1H), 2.13 (s, 2H), 2.09–1.91 (m, 5H), 1.83–1.70 (m, 3H), 1.38 (s, 6H), 1.33 (s, 3H); 13C NMR (126 MHz, CDCl3): δ 206.9, 206.5, 171.6, 171.1, 154.6, 79.5, 64.8, 57.6, 46.9, 46.8, 30.2, 29.2, 28.5, 28.4, 27.6, 27.5, 27.5, 27.3, 25.0, 25.0, 24.1, 23.6; IR (neat): νmax 2955, 2924, 1853, 1691, 1655, 1414, 1163, 1088, 848, 673 cm–1; HRMS (ESI) calcd for C16H26N2NaO4 [M + Na]+: 333.1785; found, 333.1803.

(2S)-tert-Butyl 2-((2S)-2-(1-Hydroxyethyl)pyrrolidine-1-carbonyl)pyrrolidine-1-carboxylate (22)

To a stirred solution of compound 21 (500 mg, 1.61 mmol) in dry MeOH (5 mL) was added NaBH4 (121 mg, 3.2 mmol) under a N2 atmosphere, and the reaction solution was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, sat. NH4Cl (10 mL) was added to the reaction mixture and extracted with EtOAc (3 × 10 mL). The combined organic layer was washed with brine (10 mL), dried over anhydrous Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (70% EtOAc in hexane) to afford alcohol 22 (425 mg, 85%) as a colorless oil. This was an inseparable mixture of epimers (2:1), [α]D20 −39.57 (c = 1.45, CHCl3); 1H NMR (400 MHz, CDCl3): δ 4.61–4.38 (m, 0.8H), 4.34–3.94 (m, 1.2H), 3.94–3.82 (m, 0.7H), 3.81–3.53 (m, 2.3H), 3.52–3.35 (m, 2H), 2.30–2.15 (m, 1H), 2.15–2.02 (m, 2H), 2.02–1.80 (m, 4H), 1.76–1.60 (m, 1H), 1.46 (s, 4.5H), 1.43 (s, 1.5H), 1.40 (s, 3H), 1.23–1.14 (m, 1.5H), 1.03 (d, J = 6.3 Hz, 1.5H); 13C NMR (101 MHz, CDCl3): δ 175.1, 174.9, 173.9, 173.2, 172.2, 154.5, 153.6, 79.7, 79.5, 72.0, 71.7, 69.9, 69.3, 64.9, 64.7, 64.6, 64.5, 58.5, 58.1, 57.9, 57.7, 48.2, 47.2, 46.8, 46.7, 46.5, 45.4, 30.7, 29.7, 28.4, 28.3, 28.0, 27.7, 27.7, 27.2, 24.7, 24.6, 24.5, 24.1, 23.5, 21.3, 21.2, 19.5, 17.4; IR (neat): νmax 2963, 2920, 2851, 1743, 1698, 1654, 1463, 1400, 1365, 1164, 753 cm–1; HRMS (ESI) calcd for C16H28N2NaO4 [M + Na]+: 335.1941; found, 335.1948.

Trimer 23

To a stirred solution of alcohol 22 (400 mg, 1.28 mmol) in CH2Cl2 (10 mL) was added TFA (0.27 mL, 3.5 mmol), and the reaction mixture was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (5 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the trifluoroacetate salt of deprotected 22 was afforded and used in the next reaction without purification. To a 50 mL RB flask, triflouroacetate salt of deprotected 22, dry DMF and dry DIPEA (0.9 mL, 5.12 mmol) were added at rt; to this, acid N-Boc-l-Phe (429 mg, 1.53 mmol) in dry DMF (3 mL) was added. The suspension was stirred for 10 min and HATU (730 mg, 1.92 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated to afford crude-modified dipeptide alcohol (436 mg). To a stirred solution of modified dipeptide alcohol (436 mg) in CH2Cl2 (10 mL) was added DMP (586 mg, 1.38 mmol), and the reaction solution was stirred at 0 °C for 3 h. After completion of the reaction, the reaction mixture was diluted with saturated aqueous Na2S2O3 (10 mL) and extracted with EtOAc (3 × 10 mL). The combined organic layer was washed with saturated aqueous NaHCO3 (10 mL) and brine (10 mL), dried over anhydrous Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (80% EtOAc in hexane) to give 23 (368 mg, 61%) as a colorless liquid; [α]D20 −111.96 (c = 0.65, CHCl3); mixture of rotamers, (2:1), (1H NMR, 400 MHz, CDCl3): δ 7.25–7.07 (m, 5H), 5.28–5.22 (m, 0.4H), 4.93 (dd, J = 10.3, 4.6 Hz, 0.6H), 4.7–4.4 (m, 2H), 3.88–3.78 (m, 1H), 3.73–3.61 (m, 1H), 3.61–3.50 (m, 1.4H), 3.37–3.28 (m, 0.6H), 3.11–3.01 (m, 0.5H), 3.01–2.90 (m, 1.5H), 2.76 (s, 2.2H), 2.75 (s, 0.8H), 2.18 (s, 2H), 2.17 (s, 1H), 2.14–1.96 (m, 6H), 1.93–1.86 (m, 1H), 1.83–1.75 (m, 1H), 1.24 (s, 3H), 1.07 (s, 6H); 13C NMR (126 MHz, CDCl3): δ 206.4, 206.3, 170.2, 169.6, 168.8, 155.5, 154.7, 138.0, 137.3, 129.3, 128.9, 128.2, 128.1, 126.2, 126.1, 79.8, 79.5, 64.6, 60.2, 59.2, 58.1, 57.8, 56.3, 47.0, 46.9, 46.7, 46.4, 38.4, 34.3, 34.2, 29.9, 29.0, 28.2, 28.2, 28.1, 28.0, 27.7, 27.4, 27.4, 27.3, 27.2; IR (neat): νmax 2969, 2876, 1739, 1686, 1646, 1535, 1511, 1437, 1389, 1367, 1202, 1147, 868, 772 cm–1; HRMS (ESI) calcd for C26H38N3O5 [M + H]+: 472.2806; found, 472.2824.

Tetramer 8

To a stirred solution of trimer 23 (250 mg, 0.553 mmol) in CH2Cl2 (5 mL) was added TFA (0.12 mL, 1.48 mmol), and the reaction mixture was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (5 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the trifluoroacetate salt of deprotected 23 was afforded and used in the next reaction without purification. To a 25 mL RB flask, triflouroacetate salt of deprotected 23, dry DMF and dry DIPEA (0.4 mL, 2.2 mmol) were added at rt; to this, acid N-Boc-l-Val (153 mg, 0.66 mmol) in dry DMF (3 mL) was added. The suspension was stirred for 10 min, and HATU (315 mg, 0.82 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (80% EtOAc in hexane) to give the tetramer 8 (173 mg, 56%) as a colorless oil; [α]D20 −136.20 (c = 0.55, CHCl3); mixture of rotamers, (2:1), 1H NMR (500 MHz, CDCl3): δ 7.25–7.20 (m, 2H), 7.19–7.12 (m, 3H), 5.80 (dd, J = 11.1, 4.5 Hz, 1H), 4.69 (dd, J = 8.6, 4.5 Hz, 2H), 4.54 (d, J = 10.7 Hz, 0.6H), 4.30 (d, J = 10.4 Hz, 0.4H), 3.89–3.79 (m, 1H), 3.78–3.69 (m, 1.3H), 3.68–3.56 (m, 1.7H), 3.20–3.06 (m, 2H), 3.05 (s, 1H), 3.04 (s, 2H), 2.20 (s, 3H), 2.19–2.12 (m, 4H), 2.04 (s, 3H), 2.02–1.96 (m, 2H), 1.96–1.86 (m, 1.6H), 1.86–1.77 (m, 1.4H), 1.42 (s, 6H), 1.41 (s, 3H), 0.803–0.78 (m, 3H), 0.77–0.73 (m, 3H); 13C NMR (75 MHz, CDCl3): δ 206.4, 170.6, 170.3, 169.1, 155.8, 137.1, 136.6, 129.1, 128.8, 128.5, 128.3, 126.7, 126.4, 80.0, 79.6, 64.7, 61.3, 59.7, 58.0, 54.7, 47.4, 46.8, 34.5, 30.7, 28.3, 27.5, 27.4, 26.8, 24.9, 24.8, 20.1, 19.7, 18.1, 17.7; IR (neat): νmax, 2970, 2839, 1716, 1684, 1657, 1611, 1605, 1452, 1325, 1238, 1165, 1032, 874, cm–1; HRMS (ESI) calcd for C32H48N4NaO6 [M + Na]+: 607.3466; found, 607.3486.

Trimer 6

To a stirring solution of dimer 24 (2 g, 5.3 mmol) in MeOH/H2O (7:3, 15 mL) was added LiOH·H2O (0.6 g, 16.0 mmol), and the reaction mixture was stirred at rt for 3 h. After that, the solvent (MeOH) was removed in vacuo and the resulting mixture was acidified with 1 M HCl (20 mL) to pH 4 and extracted with EtOAc (3 × 30 mL). The combined organic layer was washed with brine, dried over Na2SO4, and concentrated. The crude product was used in next step without further purification. To a stirred solution of Oep 20 (1.5 g, 7.0 mmol) in CH2Cl2 (10 mL) was added TFA (1 mL, 13.1 mmol), and the reaction mixture was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (10 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the trifluoroacetate salt of deprotected 20 was afforded and used in the next reaction without purification. To a 50 mL RB flask, triflouroacetate salt of deprotected 23, dry DMF (10 mL) and dry DIPEA (3.85 mL, 22.0 mmol) were added at 0 °C. To this, N-Boc-l-Phe-Pro-OH (2.0 g, 5.5 mmol) in dry DMF (5 mL) was added. The suspension was stirred for 10 min, and HATU (2.51 g, 6.6 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (50% EtOAc in hexane) to give trimer 6 (1.8 g, 72%) as a colorless oil; [α]D20 −20.73 (c = 1.15, CHCl3); mixture of rotamers (5:1), 1H NMR (400 MHz, CDCl3): δ 7.33–7.13 (m, 5H), 5.17 (d, J = 8.9 Hz, 0.6H), 4.80–4.61 (m, 2.4H), 3.94–3.81 (m, 1H), 3.78–3.67 (m, 1H), 3.64–3.51 (m, 2H), 3.12 (dd, J = 14.2, 4.8 Hz, 1H), 2.85–2.77 (m, 1H), 2.21 (s, 3H), 2.19–2.12 (m, 2H), 2.09–1.93 (m, 4H), 1.93–1.77 (m, 2H), 1.43 (s, 1.5H), 1.34 (s, 7.5H); 13C NMR (101 MHz, CDCl3): δ 206.3, 170.4, 170.2, 155.2, 136.4, 129.6, 128.2, 126.6, 79.4, 64.8, 57.8, 52.9, 47.2, 46.9, 38.4, 28.4, 28.2, 27.6, 27.2, 24.9, 24.8; IR (neat): νmax 3432, 2922, 1709, 1741, 1497, 1437, 1362, 1253, 1216, 1164, 1089, 1021, 864, 799 cm–1; HRMS (ESI) calcd for C25H36N3O5 [M + H]+: 458.2649; found, 458.2641.

N-Boc-l-Val-l-Val-l-Val-OMe (26)

To a stirring solution of dimer 25 (3.5 g, 9.1 mmol) in MeOH/H2O (7:3, 50 mL) was added LiOH·H2O (1.2 g, 27.0 mmol), and the reaction mixture was stirred at rt for 3 h. After that, the solvent (MeOH) was removed in vacuo and the resulting mixture was acidified with 1 M HCl (20 mL) to pH 4 and extracted with EtOAc (3 × 30 mL). The combined organic layer was washed with brine, dried over Na2SO4, and concentrated. The crude product was used in next step without further purification. To a stirred solution of l-valine methyl ester hydrochloride (1.9 g, 11.0 mmol) in dry CH2Cl2 (10 mL) was added DIPEA (5 mL, 29.0 mmol) at 0 °C and stirred for 10 min. The above crude product was dissolved in dry CH2Cl2 (25 mL), and EDC·HCl (2.0 g, 10.4 mmol) and HOBt (1.5 g, 10.4 mmol) were added to the above suspension; after stirring for 6 h at rt, the reaction mixture was diluted with CH2Cl2 (50 mL) and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (50% EtOAc in hexane) to give the trimer 26 (3.1 g, 75%) as a white solid; mp 156–158 °C; [α]D20 −33.69 (c = 1.65, CHCl3); 1H NMR (300 MHz, CDCl3): δ 7.06 (s, 1H), 6.88 (s, 1H), 5.44 (s, 1H), 4.52 (dd, J = 8.7, 5.3 Hz, 1H), 4.38 (t, J = 8.1 Hz, 1H), 3.95 (t, J = 7.7 Hz, 1H), 3.71 (s, 3H), 2.22–1.95 (m, 3H), 1.41 (s, 9H), 0.90 (s, 9H), 0.87 (d, J = 9.9 Hz, 9H); 13C NMR (126 MHz, CDCl3): δ 172.1, 172.0, 171.3, 155.9, 79.6, 60.2, 58.6, 57.1, 52.0, 30.9, 30.8, 30.7, 28.3, 19.2, 19.1, 18.9, 18.3, 18.1, 17.8; IR (neat): νmax 3294, 2964, 2925, 1739, 1691, 1644, 1519, 1462, 1369, 1299, 1214, 1166, 1090, 1016, 926, 875 cm–1; HRMS (ESI) calcd for C21H39N3NaO6 [M + Na]+: 452.2731; found, 452.2733.

N-Boc-l-Ala-l-Pro-l-Val-l-Val-l-Val-OMe (5)

To a stirred solution of trimer 26 (2.0 g, 4.2 mmol) in CH2Cl2 (15 mL) was added TFA (0.9 mL, 11.8 mmol), and the reaction mixture was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (10 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the trifluoroacetate salt of deprotected 26 was afforded and used in the next reaction without purification. To a 25 mL RB flask, triflouroacetate salt of deprotected 26, dry DMF (10 mL), and dry DIPEA (2.6 mL, 15.0 mmol) were added at rt. To this, N-Boc-l-Ala-Pro-OH 27 (1.0 g, 3.7 mmol) in dry DMF (5 mL) was added. The suspension was stirred for 10 min, and HATU (1.7 g, 4.5 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (50% EtOAc in hexane) to give pentamer 5 (1.3 g, 64%) as a white solid; mp = 167–169 °C; [α]D20 −84.15 (c = 0.8, CHCl3); 1H NMR (500 MHz, CDCl3): δ 7.47 (s, 1H), 6.92 (d, J = 33.7 Hz, 1H), 6.78 (d, J = 18.3 Hz, 1H), 5.50 (d, J = 7.3 Hz, 1H), 4.68 (d, J = 5.2 Hz, 1H), 4.55–4.46 (m, 2H), 4.41–4.33 (m, 1H), 4.32–4.23 (m, 1H), 3.72 (s, 3H), 3.71–3.67 (m, 1H), 3.61–3.55 (m, 1H), 2.21–2.06 (m, 4H), 2.04–1.88 (m, 3H), 1.41 (s, 9H), 1.31 (d, J = 7.3, Hz, 3H), 0.93 (d, J = 3.9 Hz, 3H), 0.91 (d, J = 3.5 Hz, 3H), 0.90 (d, J = 4.0 Hz, 3H), 0.89–0.87 (m, 6H), 0.87 (d, J = 2.0 Hz, 3H); 13C NMR (126 MHz, CDCl3): δ 173.1, 172.1, 171.3, 171.2, 171.0, 155.2, 79.7, 59.9, 58.9, 58.7, 57.1, 52.1, 47.8, 47.3, 31.0, 30.7, 30.5, 28.3, 27.5, 25.2, 19.3, 19.2, 18.9, 18.7, 18.2, 17.8, 17.7; IR (neat): νmax 3289, 3074, 2962, 2923, 1740, 1703, 1640, 1544, 1452, 1370, 1211, 1165, 1093, 1057, 1022, 924, 864, 754 cm–1; HRMS (ESI) calcd for C29H51N5NaO8 [M + Na]+: 620.3630; found, 620.3614.

Octapeptide 3a

To a stirring solution of pentamer 5 (1 g, 1.6 mmol) in MeOH/H2O (7:3, 10 mL) was added LiOH·H2O (211 mg, 5.0 mmol), and the reaction mixture was stirred at rt for 3 h. After that, the solvent (MeOH) was removed in vacuo and the resulting mixture was acidified with 1 M HCl (20 mL) to pH 4 and extracted with EtOAc (3 × 30 mL). The combined organic layer was washed with brine, dried over Na2SO4, and concentrated. The crude product was used in next step without further purification.

To a stirred solution of trimer 6 (1.0 g, 2.1 mmol) in CH2Cl2 (10 mL) was added TFA (0.3 mL, 4.3 mmol), and the reaction mixture was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (10 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the trifluoroacetate salt of deprotected 6 was afforded and used in the next reaction without purification. To a 50 mL RB flask, triflouroacetate salt of deprotected 6, dry DMF (5 mL), and dry DIPEA (1.19 mL, 6.8 mmol) were added at rt. To this, pentamer acid (1.0 g, 1.7 mmol) in dry DMF (5 mL) was added. The suspension was stirred for 10 min, and HATU (782 mg, 2.0 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (20 mL) and sat. NaHCO3 (20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (80% EtOAc in hexane) to give octapeptide 3a (822 mg, 52%) as a white solid; mp = 196–198 °C; [α]D20 −67.0 (c = 0.3, CHCl3); 1H NMR (500 MHz, CDCl3): δ 7.60 (br s, 1H), 7.29 (d, J = 7.3 Hz, 1H), 7.24 (d, J = 7.9 Hz, 1H), 7.22–7.13 (m, 5H), 6.89 (br s, 1H), 5.47 (br s, 1H), 5.07–4.97 (m, 1H), 4.75–4.69 (m, 1H), 4.65 (dd, J = 8.6, 4.5 Hz, 1H), 4.52 (d, J = 7.4 Hz, 1H), 4.45–4.20 (m, 4H), 3.88–3.69 (m, 3H), 3.65–3.51 (m, 3H), 3.17–3.06 (m, 1H), 2.93–2.84 (m, 1H), 2.20 (s, 3H), 2.17–2.10 (m, 5H), 2.08–1.94 (m, 8H), 1.83–1.72 (m, 2H), 1.43 (s, 9H), 1.34 (d, J = 6.5 Hz, 3H), 0.93–0.79 (m, 18H); 13C NMR (101 MHz, CDCl3): δ 206.4, 173.0, 172.5, 171.6, 171.5, 170.8, 170.7, 170.2, 155.2, 136.2, 129.8, 129.5, 128.3, 126.6, 79.7, 66.1, 64.9, 60.1, 58.0, 57.9, 51.8, 51.6, 47.9, 47.4, 46.9, 37.9, 31.2, 30.7, 30.7, 29.7, 28.4, 27.6, 27.2, 25.2, 24.9, 19.4, 19.3, 18.7, 18.6, 18.5, 18.2; IR (neat): νmax 3295, 3078, 2916, 2901, 1752, 1703, 1641, 1547, 1452, 1375, 1211, 1165, 1098, 1057, 1027, 924, 867, 754 cm–1; HRMS (ESI) calcd for C48H74N8NaO10 [M + Na]+: 945.5420; found, 945.5437.

Desmethyl Jahanyne 1a

To a stirred solution of octapeptide 3a (200 mg, 0.2 mmol), in CH2Cl2 (2 mL) was added TFA (0.03 mL, 0.4 mmol), and the reaction solution was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (1 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the triflouroacetate salt of deprotected 24 was afforded and used in the next reaction without purification. To a 10 mL RB flask, triflouroacetate salt of deprotected 3a, dry DMF, and dry DIPEA (0.12 mL, 0.7 mmol) were added at rt. To this, acid 2 (35 mg, 0.17 mmol) in dry DMF (3 mL) was added. The suspension was stirred for 10 min, and HATU (79 mg, 0.21 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (5 mL) and sat. NaHCO3 (5 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (6% CHCl3 in MeOH) followed by HPLC [Luna C8 (250 × 4.6 mm, 5.0 μm); flow rate 1.0 mL/min; detection, UV 210 nm; solvent; 70% CH3CN in 0.1% formic acid] give the desmethyl jahanyne 1a (78 mg, 44%) yellow solid; mp = 178–180 °C; [α]D20 −50.80 (c = 1.25, CHCl3); 1H NMR (500 MHz, CDCl3): δ 7.33–7.13 (m, 5H), 7.03 (br s 1H), 6.97–6.78 (m, 1H), 6.72–6.58 (m, 1H), 5.02–4.87 (m, 1H), 4.84–4.70 (m, 1H), 4.66 (dt, J = 10.6, 5.2 Hz, 1H), 4.61–4.55 (m, 1H), 4.53–4.29 (m, 2H), 4.28–3.99 (m, 2H), 3.98–3.52 (m, 5H), 3.49–2.81 (m, 3H), 2.36–2.21 (m, 3H), 2.20 (s, 3H), 2.18–2.09 (m, 6H), 2.09–2.03 (m, 3H), 2.02–1.95 (m, 4H), 1.94 (t, J = 2.8 Hz, 1H), 1.92–1.78 (m, 2H), 1.53–1.38 (m, 5H), 1.37–1.24 (m, 6H), 1.17–1.09 (m, 1H), 1.06 (d, J = 6.5 Hz, 3H), 1.04–0.76 (m, 18H), 0.74 (d, J = 6.7 Hz, 1.8 H), 0.67 (d, J = 6.7 Hz, 1.2H); 13C NMR (101 MHz, CDCl3): δ 206.2, 176.4, 174.0, 172.8, 171.2, 170.6, 170.3, 170.0, 169.9, 136.3, 129.4, 129.2, 128.4, 126.8, 84.6, 68.2, 64.9, 60.2, 59.7, 58.3, 58.0, 51.9, 47.4, 46.9, 46.4, 41.3, 38.8, 38.7, 38.0, 37.9, 37.0, 36.4, 31.1, 31.0, 30.4, 28.6, 28.3, 27.5, 27.2, 26.0, 25.2, 25.0, 24.8, 20.7, 19.4, 19.3, 19.2, 18.5, 18.4, 18.0, 17.7, 17.5, 17.4; IR (neat): νmax 3280, 2960, 2922, 1720, 1632, 1533, 1445, 1437, 1375, 1217, 1160, 1096, 919, 801, 752 cm–1; HRMS (ESI) calcd for C55H84N8NaO9 [M + Na]+: 1023.6253; found, 1023.6273.

Compound 28

To a stirred solution of tetramer 7 (50 mg, 0.09 mmol), in CH2Cl2 (2 mL) was added TFA (0.2 mL, 0.25 mmol), and the reaction solution was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (1 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the triflouroacetate salt of deprotected 2 was afforded which was used in the next reaction without purification. To a 10 mL RB flask, triflouroacetate salt of deprotected 19, dry DMF, and dry DIPEA (0.6 mL, 0.37 mmol) were added at rt. To this, acid 2 (21 mg, 0.11 mmol) in dry DMF (3 mL) was added. The suspension was stirred for 10 min, and HATU (52.7 mg, 0.13 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (5 mL) and sat. NaHCO3 (5 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (70% EtOAc in hexane) followed by HPLC [CSH XSelect (150 × 4.6 mm, 5.0 μm); flow rate 1.0 mL/min; detection, UV 210 nm; solvent; 75% CH3OH in 0.1% formic acid] gave 28 (23.8 mg, 42%) as colorless liquid; [α]D20 −136.30 (c = 0.3, CHCl3); mixture of rotamers, (4:1), 1H NMR (400 MHz, CDCl3): δ 5.51–5.44 (m, 1H), 5.14 (d, J = 10.9 Hz, 0.8H), 5.06 (d, J = 10.6 Hz, 0.2H), 4.85 (d, J = 10.6 Hz, 1H), 4.80 (dd, J = 7.9, 3.6 Hz, 0.8H), 4.39 (d, J = 9.9 Hz, 0.2H), 3.75–3.59 (m, 2H), 3.69 (s, 2.3H), 3.67 (s, 0.7H), 3.09 (s, 2.2H), 3.05 (s, 0.8H), 3.03 (s, 2.3H), 3.01 (s, 2.2H), 3.01 (s, 0.7H), 2.86–2.78 (m, 1H), 2.77 (s, 0.8H), 2.43–2.20 (m, 2H), 2.17 (td, J = 6.9, 2.6 Hz, 2H), 2.14–1.98 (m, 2H), 1.93 (t, J = 2.7 Hz, 1H), 1.91–1.86 (m, 1H), 1.75–1.68 (m, 1H), 1.52–1.35 (m, 7H), 1.29 (d, J = 7.0 Hz, 3H), 1.27–1.24 (m, 2H), 1.08 (d, J = 6.7 Hz, 3H), 1.05 (d, J = 6.5 Hz, 0.8H), 1.01 (d, J = 6.5 Hz, 2.2H), 0.93 (d, J = 6.7 Hz, 3H), 0.90 (d, J = 6.5 Hz, 3H), 0.86 (d, J = 5.6 Hz, 3H), 0.76 (d, J = 6.7 Hz, 3H); 13C NMR (75 MHz, CDCl3): δ 177.0, 172.6, 171.6, 171.3, 170.2, 84.5, 68.2, 64.4, 61.4, 58.5, 58.0, 56.9, 52.0, 51.8, 50.0, 47.0, 41.1, 40.9, 36.7, 33.5, 31.6, 30.5, 30.4, 30.2, 29.7, 28.6, 28.4, 27.4, 27.1, 26.0, 24.7, 19.9, 19.5, 19.1, 18.6, 18.3, 17.1, 14.2, 1.0; IR (neat): νmax 3325, 1756, 1673, 1628, 1624, 2973, 2876, 2839, 1740, 1686, 1651, 1535, 1442, 1150, 1081, 868, 771 cm–1; HRMS (ESI) calcd for C34H59N4O6 [M + H]+: 619.4429; found, 619.4455.

Compound 29

To a stirred solution of tetramer 8 (50 mg, 0.08 mmol), in CH2Cl2 (2 mL) was added TFA (0.2 mL, 0.23 mmol), and the reaction solution was stirred at 0 °C for 1 h and at rt for 1 h. After completion of the reaction, benzene (1 mL) was added to the reaction mixture and concentrated. After repetition of this operation five times, the triflouroacetate salt of deprotected 24 was afforded and used in the next reaction without purification. To a 10 mL RB flask, triflouroacetate salt of deprotected 24, dry DMF, and dry DIPEA (0.6 mL, 0.34 mmol) were added at rt. To this, acid 2 (20 mg, 0.1 mmol) in dry DMF (3 mL) was added. The suspension was stirred for 10 min, and HATU (48.4 mg, 0.12 mmol) was added under N2 atmosphere. After stirring for 12 h, the reaction mixture was diluted with EtOAc and washed with 10% aq citric acid (5 mL) and sat. NaHCO3 (5 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated. The residue was purified by column chromatography on silica gel (80% EtOAc in hexane) followed by HPLC [Luna C8 (250 × 4.6 mm, 5.0 μm); flow rate 1.0 mL/min; detection, UV 210 nm; solvent; 70% CH3CN in 0.1% formic acid] give the 29 (36.8 mg, 65%) as colorless liquid; [α]D20 −185.65 (c = 1.35, CHCl3); 1H NMR (500 MHz, CDCl3): δ 7.25–7.18 (m, 2H), 7.17–7.12 (m, 3H), 5.91–5.85 (m, 1H), 5.02 (dd, J = 10.5, 3.2 Hz, 1H), 4.73–4.59 (m, 2H), 3.89–3.70 (m, 2H), 3.67–3.54 (m, 2H), 3.15–2.98 (m, 2H), 2.93 (s, 3H), 2.54–2.43 (m, 1H), 2.17 (s, 3H), 2.17–2.14 (m, 3H), 2.13 (s, 3H), 2.12–1.92 (m, 6H), 1.91–1.89 (m, 1H), 1.88–1.75 (m, 2H), 1.47–1.35 (m, 5H), 1.29–1.19 (m, 3H), 1.07–1.00 (m, 1H), 0.93–0.88 (m, 3H), 0.83–0.79 (m, 6H), 0.68–0.64 (m, 3H); 13C NMR (101 MHz, CDCl3): δ 206.4, 176.4, 170.3, 170.2, 168.9, 137.0, 129.1, 128.2, 126.4, 84.4, 68.1, 64.7, 58.0, 58.0, 54.2, 47.4, 46.8, 40.9, 36.3, 34.4, 33.2, 30.4, 30.1, 29.0, 28.6, 28.2, 27.4, 27.3, 26.6, 25.9, 24.8, 24.7, 19.8, 19.5, 18.3, 17.9, 17.1; IR (neat): νmax 3312, 2958, 2925, 2835, 1721, 1691, 1606, 1443, 1366, 1165, 751, 700 cm–1; HRMS (ESI) calcd. for C39H58N4NaO5 [M + Na]+: 685.4299; found, 685.4293.