L Hulshof1, D P Hack2, Q C J Hasnoe3, B Dontje1, I Jakasa4, C Riethmüller5, W H I McLean6, W M C van Aalderen1, B Van't Land7,8, S Kezic3, A B Sprikkelman9, M A Middelkamp-Hup2. 1. Emma Children's Hospital Amsterdam UMC, University of Amsterdam, Department of Paediatric Respiratory Medicine and Allergy, Amsterdam, the Netherlands. 2. Department of Dermatology, Amsterdam Public Health Research Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands. 3. Coronel Institute of Occupational Health, Amsterdam Public Health Research Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands. 4. Department of Chemistry and Biochemistry, Laboratory for Analytical Chemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia. 5. Serend-ip GmbH, Munster, Germany. 6. Centre for Dermatology and Genetic Medicine, Division of Molecular Medicine, Colleges of Life Sciences and Medicine, Dentistry & Nursing, University of Dundee, Dundee, U.K. 7. University Medical Centre Utrecht, Wilhelmina Children's Hospital, Department of Paediatric Immunology, Utrecht, the Netherlands. 8. Nutricia Research, Department of Immunology, Utrecht, the Netherlands. 9. University of Groningen, University Medical Centre Groningen, Beatrix Children's Hospital, Department of Paediatric Pulmonology and Paediatric Allergology, Groningen, the Netherlands.
Abstract
BACKGROUND: Atopic dermatitis (AD) affects children of all skin types. Most research has focused on light skin types. Studies investigating biomarkers in people with AD with dark skin types are lacking. OBJECTIVES: To explore skin barrier and immune response biomarkers in stratum corneum (SC) tape strips from children with AD with different skin types. METHODS: Tape strips were collected from lesional and nonlesional forearm skin of 53 children with AD and 50 controls. We analysed 28 immunomodulatory mediators, and natural moisturizing factors (NMF) and corneocyte morphology. RESULTS: Interleukin (IL)-1β, IL-18, C-X-C motif chemokine (CXCL) 8 (CXCL8), C-C motif chemokine ligand (CCL) 22 (CCL22), CCL17, CXCL10 and CCL2 were significantly higher (P < 0·05) in lesional AD skin compared with nonlesional AD skin; the opposite trend was seen for IL-1α. CXCL8, CCL2 and CCL17 showed an association with objective SCORing Atopic Dermatitis score. NMF levels showed a gradual decrease from healthy skin to nonlesional and lesional AD skin. This gradual decreasing pattern was observed in skin type II but not in skin type VI. Skin type VI showed higher NMF levels in both nonlesional and lesional AD skin than skin type II. Corneocyte morphology was significantly different in lesional AD skin compared with nonlesional AD and healthy skin. CONCLUSIONS: Minimally invasive tape-stripping is suitable for the determination of many inflammatory mediators and skin barrier biomarkers in children with AD. This study shows differences between children with AD with skin type II and skin type VI in NMF levels, suggesting that some aspects of pathophysiological mechanisms may differ in AD children with light versus dark skin types.
BACKGROUND:Atopic dermatitis (AD) affects children of all skin types. Most research has focused on light skin types. Studies investigating biomarkers in people with AD with dark skin types are lacking. OBJECTIVES: To explore skin barrier and immune response biomarkers in stratum corneum (SC) tape strips from children with AD with different skin types. METHODS: Tape strips were collected from lesional and nonlesional forearm skin of 53 children with AD and 50 controls. We analysed 28 immunomodulatory mediators, and natural moisturizing factors (NMF) and corneocyte morphology. RESULTS: Interleukin (IL)-1β, IL-18, C-X-C motif chemokine (CXCL) 8 (CXCL8), C-C motif chemokine ligand (CCL) 22 (CCL22), CCL17, CXCL10 and CCL2 were significantly higher (P < 0·05) in lesional AD skin compared with nonlesional AD skin; the opposite trend was seen for IL-1α. CXCL8, CCL2 and CCL17 showed an association with objective SCORing Atopic Dermatitis score. NMF levels showed a gradual decrease from healthy skin to nonlesional and lesional AD skin. This gradual decreasing pattern was observed in skin type II but not in skin type VI. Skin type VI showed higher NMF levels in both nonlesional and lesional AD skin than skin type II. Corneocyte morphology was significantly different in lesional AD skin compared with nonlesional AD and healthy skin. CONCLUSIONS: Minimally invasive tape-stripping is suitable for the determination of many inflammatory mediators and skin barrier biomarkers in children with AD. This study shows differences between children with AD with skin type II and skin type VI in NMF levels, suggesting that some aspects of pathophysiological mechanisms may differ in ADchildren with light versus dark skin types.
Authors: Jocelyn M Biagini Myers; Michael G Sherenian; Asel Baatyrbek Kyzy; Rosario Alarcon; Amen An; Zachary Flege; David Morgan; Tammy Gonzalez; Mariana L Stevens; Hua He; John W Kroner; Daniel Spagna; Brittany Grashel; Lisa J Martin; Andrew B Herr; Gurjit K Khurana Hershey Journal: J Allergy Clin Immunol Pract Date: 2020-04-14
Authors: Ana B Pavel; Yael Renert-Yuval; Jianni Wu; Ester Del Duca; Aisleen Diaz; Rachel Lefferdink; Milie M Fang; Talia Canter; Stephanie M Rangel; Ning Zhang; James G Krueger; Amy S Paller; Emma Guttman-Yassky Journal: Allergy Date: 2020-08-20 Impact factor: 14.710
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Authors: Anne J Keurentjes; Kornelis D de Witt; Ivone Jakasa; Lars Rüther; Patrick M J H Kemperman; Sanja Kezic; Christoph Riethmüller Journal: Exp Dermatol Date: 2020-03-13 Impact factor: 3.960