Migyeong Geum1,2, Ha-Jung Kim3,2. 1. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea. 2. BK21 Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea. 3. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea; kimhj614@jnu.ac.kr.
Abstract
BACKGROUND/AIM: No study has investigated skin barrier dysfunction with systemic diseases in veterinary medicine. We investigated the mechanism of disturbed skin barrier function in dogs with internal diseases. MATERIALS AND METHODS: Healthy controls and dogs with systemic diseases were enrolled in three different disease groups: malignant tumor, hyperadrenocorticism and kidney disease. Transepidermal water loss (TEWL), serum levels of five selective pro-inflammatory cytokines and claudin-1, and complete blood count were measured. RESULTS: TEWL was significantly increased in the malignant tumor group while serum claudin-1 concentrations were significantly lower compared to controls. Tumor necrosis factor-α was also significantly increased in the cancer group. In addition, the malignant tumor group showed significantly higher monocyte chemotactic protein-1 after chemotherapy, but lower interleukin-6 levels, compared to dogs with no chemotherapy. CONCLUSION: Skin barrier function was decreased in dogs with malignant tumors compared to dogs with other systemic diseases by oxidative stress and a reduction in tight junction proteins. Copyright
BACKGROUND/AIM: No study has investigated skin barrier dysfunction with systemic diseases in veterinary medicine. We investigated the mechanism of disturbed skin barrier function in dogs with internal diseases. MATERIALS AND METHODS: Healthy controls and dogs with systemic diseases were enrolled in three different disease groups: malignant tumor, hyperadrenocorticism and kidney disease. Transepidermal water loss (TEWL), serum levels of five selective pro-inflammatory cytokines and claudin-1, and complete blood count were measured. RESULTS: TEWL was significantly increased in the malignant tumor group while serum claudin-1 concentrations were significantly lower compared to controls. Tumor necrosis factor-α was also significantly increased in the cancer group. In addition, the malignant tumor group showed significantly higher monocyte chemotactic protein-1 after chemotherapy, but lower interleukin-6 levels, compared to dogs with no chemotherapy. CONCLUSION: Skin barrier function was decreased in dogs with malignant tumors compared to dogs with other systemic diseases by oxidative stress and a reduction in tight junction proteins. Copyright
Authors: Vanessa S Machado; Leandro Z Crivellenti; Nathieli B Bottari; Alexandre A Tonin; Luana P Pelinson; Sofia Borin-Crivellenti; Aureo E Santana; Vanessa D Torbitz; Rafael N Moresco; Thiago Duarte; Marta M M F Duarte; Maria Rosa C Schetinger; Vera M Morsch; Jeandre A Jaques; Mirela Tinucci-Costa; Aleksandro S Da Silva Journal: Pathol Res Pract Date: 2015-06-26 Impact factor: 3.250