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Literature DB >> 2997483

In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.

Abstract

Development of a novel infectious cDNA assay is described for human rhinovirus type 14. A full-length cDNA clone of the human rhinovirus type 14 genome RNA was assembled and transcribed in vitro by using the SP6 transcription system. Transfection of HeLa cells with the nascent RNA resulted in the production of rhinovirus indistinguishable from the parental virus by both immunological and polyacrylamide gel analysis.

Entities: Chemical Disease

Mesh：

Substances：
RNA, Viral
DNA

Year: 1985 PMID： 2997483 PMCID： PMC252623

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

17 in total

1. Biophysical studies of a rhinovirus. Extraction and assay of infectious ribonucleic acid.

Authors: N J Dimmock
Journal: Nature Date: 1966-02-19 Impact factor: 49.962

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. The complete nucleotide sequence of a common cold virus: human rhinovirus 14.

Authors: G Stanway; P J Hughes; R C Mountford; P D Minor; J W Almond
Journal: Nucleic Acids Res Date: 1984-10-25 Impact factor: 16.971

4. Cloned infectious complementary DNA of the poliovirus Sabin 1 genome: biochemical and biological properties of the recovered virus.

Authors: T Omata; M Kohara; Y Sakai; A Kameda; N Imura; A Nomoto
Journal: Gene Date: 1984-12 Impact factor: 3.688

5. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors: D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal: Nucleic Acids Res Date: 1984-09-25 Impact factor: 16.971

6. Cloned poliovirus complementary DNA is infectious in mammalian cells.

Authors: V R Racaniello; D Baltimore
Journal: Science Date: 1981-11-20 Impact factor: 47.728

7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors: J Vieira; J Messing
Journal: Gene Date: 1982-10 Impact factor: 3.688

8. Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.

Authors: T Takegami; R J Kuhn; C W Anderson; E Wimmer
Journal: Proc Natl Acad Sci U S A Date: 1983-12 Impact factor: 11.205

9. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.

Authors: B A Parker; G R Stark
Journal: J Virol Date: 1979-08 Impact factor: 5.103

10. Rhinoviruses.

Authors: J M Gwaltney
Journal: Yale J Biol Med Date: 1975-03

42 in total

1. In vivo analysis of the promoter structure of the influenza virus RNA genome using a transfection system with an engineered RNA.

Authors: K Yamanaka; N Ogasawara; H Yoshikawa; A Ishihama; K Nagata
Journal: Proc Natl Acad Sci U S A Date: 1991-06-15 Impact factor: 11.205

In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.

1. Biophysical studies of a rhinovirus. Extraction and assay of infectious ribonucleic acid.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. The complete nucleotide sequence of a common cold virus: human rhinovirus 14.

4. Cloned infectious complementary DNA of the poliovirus Sabin 1 genome: biochemical and biological properties of the recovered virus.

5. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

6. Cloned poliovirus complementary DNA is infectious in mammalian cells.

7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

8. Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.

9. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.

10. Rhinoviruses.

1. In vivo analysis of the promoter structure of the influenza virus RNA genome using a transfection system with an engineered RNA.

2. Conservation of RNA-protein interactions among picornaviruses.

3. Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli.

4. Role of 3'-end sequences in infectivity of poliovirus transcripts made in vitro.

5. Evidence for the direct involvement of the rhinovirus canyon in receptor binding.

6. Mutational analysis of the genome-linked protein VPg of poliovirus.

7. Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.

8. Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus.

9. Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast.

Review 10. mRNA-based therapeutics--developing a new class of drugs.