Literature DB >> 6324172

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.

T Takegami, R J Kuhn, C W Anderson, E Wimmer.   

Abstract

A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.

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Year:  1983        PMID： 6324172      PMCID： PMC389968          DOI： 10.1073/pnas.80.24.7447

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  32 in total

1.  An enzymatic activity in uninfected cells that cleaves the linkage between poliovirion RNA and the 5' terminal protein.

Authors:  V Ambros; R F Pettersson; D Baltimore
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

2.  The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.

Authors:  A Nomoto; B Detjen; R Pozzatti; E Wimmer
Journal:  Nature       Date:  1977-07-21       Impact factor: 49.962

3.  Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.

Authors:  J B Flanegan; D Baltimore
Journal:  J Virol       Date:  1979-01       Impact factor: 5.103

4.  O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.

Authors:  P G Rothberg; T J Harris; A Nomoto; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  The role of cytoplasmic membranes in poliovirus biosynthesis.

Authors:  L A Caliguiri; I Tamm
Journal:  Virology       Date:  1970-09       Impact factor: 3.616

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Dependence of the activity of the poliovirus replicase on the host cell protein.

Authors:  A Dasgupta; P Zabel; D Baltimore
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

8.  Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded.

Authors:  K Dorsch-Häsler; Y Yogo; E Wimmer
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

9.  Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA.

Authors:  A Dasgupta; M H Baron; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

10.  A protein covalently linked to poliovirus genome RNA.

Authors:  Y F Lee; A Nomoto; B M Detjen; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  80 in total

1.  Primer-dependent synthesis by poliovirus RNA-dependent RNA polymerase (3D(pol)).

Authors:  V Rodriguez-Wells; S J Plotch; J J DeStefano
Journal:  Nucleic Acids Res       Date:  2001-07-01       Impact factor: 16.971

2.  Recombination of poliovirus RNA proceeds in mixed replication complexes originating from distinct replication start sites.

Authors:  Denise Egger; Kurt Bienz
Journal:  J Virol       Date:  2002-11       Impact factor: 5.103

3.  Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol.

Authors:  P L Rodriguez; L Carrasco
Journal:  J Virol       Date:  1992-04       Impact factor: 5.103

4.  cis-acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication.

Authors:  C Giachetti; S S Hwang; B L Semler
Journal:  J Virol       Date:  1992-10       Impact factor: 5.103

5.  Manganese-dependent polioviruses caused by mutations within the viral polymerase.

Authors:  Shane Crotty; David Gohara; Devin K Gilligan; Sveta Karelsky; Craig E Cameron; Raul Andino
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103

6.  A temperature-sensitive defect of enterovirus 70 is located at the uridylylation of the genome-linked protein VPg in vitro.

Authors:  N Takeda; K Miyamura; T Takegami; S Yamazaki
Journal:  Virus Genes       Date:  1989-08       Impact factor: 2.332

7.  Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.

Authors:  P Argos; G Kamer; M J Nicklin; E Wimmer
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

8.  Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis.

Authors:  S G Sawicki; D L Sawicki
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

9.  A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.

Authors:  K Shiroki; T Ishii; T Aoki; M Kobashi; S Ohka; A Nomoto
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103

10.  Poliovirus protein 3AB forms a complex with and stimulates the activity of the viral RNA polymerase, 3Dpol.

Authors:  S J Plotch; O Palant
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103
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