Literature DB >> 6402497

Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.

P Gay, D Le Coq, M Steinmetz, E Ferrari, J A Hoch.   

Abstract

A clone bearing the structural gene sacB, coding for the exoenzyme levansucrase, was isolated from a library of Bacillus subtilis DNA that was cloned in phage lambda charon 4A on the basis of the transforming activity of the chimeric DNA. This lambda clone also was found to contain the sacR and smo loci. Subcloning the sacB-sacR region in plasmid pBR325 resulted in a clone which directed levansucrase synthesis in Escherichia coli. The nucleotide sequence coding for the secreted protein was localized on the physical map of the cloned DNA.

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Year:  1983        PMID： 6402497      PMCID： PMC221793          DOI： 10.1128/jb.153.3.1424-1431.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  33 in total

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Authors:  J Berthou; A Laurent; E Lebrun; R van Rapenbusch
Journal:  J Mol Biol       Date:  1974-01-05       Impact factor: 5.469

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Authors:  R Chambert; G Treboul; R Dedonder
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5.  Identification of the structural gene of levansucrase in Bacillus subtilis Marburg.

Authors:  J A Lepesant; J Lepesant-Kejzlarová; M Pascal; F Kunst; A Billault; R Dedonder
Journal:  Mol Gen Genet       Date:  1974-02-06

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Authors:  J A Lepesant; F Kunst; J Lepesant-Kejzlarová; R Dedonder
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Authors:  S Borenstein; E Ephrati-Elizur
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9.  Levansucrase of Bacillus subtilis : reexamination of some physical and chemical properties.

Authors:  G Gonzy-Treboul; R Chambert; R Dedonder
Journal:  Biochimie       Date:  1975       Impact factor: 4.079

10.  Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis.

Authors:  G F Grant; M I Simon
Journal:  J Bacteriol       Date:  1969-07       Impact factor: 3.490
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  68 in total

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7.  Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.

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