Milena Villarini1, Sara Levorato1, Tania Salvatori1, Elisabetta Ceretti2, Sara Bonetta3, Annalaura Carducci4, Tiziana Grassi5, Samuele Vannini1, Francesco Donato2, Silvia Bonetta3, Marco Verani4, Antonella de Donno5, Silvia Bonizzoni6, Alberto Bonetti7, Massimo Moretti8, Umberto Gelatti2. 1. Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122, Perugia, Italy. 2. Department of Medical and Surgical Specialties, Radiological Science and Public Health, University of Brescia, 11 Viale Europa, 25123, Brescia, Italy. 3. Department of Public Health and Pediatrics, University of Torino, 94 Piazza Polonia, 10126, Torino, Italy. 4. Department of Biology, University of Pisa, 13 Via Ghini, 56126, Pisa, Italy. 5. Department of Biological and Environmental Science and Technology, University of Salento, 165 Via Provinciale Lecce-Monteroni, 73100, Lecce, Italy. 6. Comune di Brescia, 1 Piazza Repubblica, 25100, Brescia, Italy. 7. Centro Servizi Multisettoriale e Tecnologico - CSMT Gestione S.c.a.r.l., 45 Via Branze, 25123, Brescia, Italy. 8. Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122, Perugia, Italy. Electronic address: massimo.moretti@unipg.it.
Abstract
BACKGROUND: Recent data support the hypothesis that genetic damage occurring early in life during childhood can play an important role in the development of chronic diseases in adulthood, including cancer. OBJECTIVES: The objective of this paper, part of the MAPEC_LIFE project, is to describe the frequency of micronuclei and meta-nuclear alterations in exfoliated buccal cells of 6-8year-old Italian children recruited in five Italian towns (i.e., Brescia, Torino, Pisa, Perugia and Lecce) with different air pollution levels. METHODS: About 200 children per town were recruited from primary schools. Biological samples were collected twice from the same children, in two different seasons (winter 2014-15 and late spring 2015). Cytogenetic damage was evaluated by the buccal micronucleus cytome assay. RESULTS: Overall,n = 1046 children represent the final cohort of the MAPEC_LIFE study. On the whole, the results showed a higher mean MN frequency in winter (0.42 ± 0.54‰) than late-spring (0.22 ± 0.34‰). MN frequency observed among the five Italian towns showed a trend that follows broadly the levels of air pollution in Italy: the highest MN frequency was observed in Brescia during both seasons, the lowest in Lecce (winter) and Perugia (late-spring). CONCLUSIONS: To the best of our knowledge, the number of recruited children included in the analysis (n = 1046) is the highest compared to previous studies evaluating the frequency of MN in exfoliated buccal cells so far. MN frequency was associated with winter season and living in towns at various levels of air pollution, suggesting an important role of this exposure in determining early cytogenetic effects.
BACKGROUND: Recent data support the hypothesis that genetic damage occurring early in life during childhood can play an important role in the development of chronic diseases in adulthood, including cancer. OBJECTIVES: The objective of this paper, part of the MAPEC_LIFE project, is to describe the frequency of micronuclei and meta-nuclear alterations in exfoliated buccal cells of 6-8year-old Italian children recruited in five Italian towns (i.e., Brescia, Torino, Pisa, Perugia and Lecce) with different air pollution levels. METHODS: About 200 children per town were recruited from primary schools. Biological samples were collected twice from the same children, in two different seasons (winter 2014-15 and late spring 2015). Cytogenetic damage was evaluated by the buccal micronucleus cytome assay. RESULTS: Overall,n = 1046 children represent the final cohort of the MAPEC_LIFE study. On the whole, the results showed a higher mean MN frequency in winter (0.42 ± 0.54‰) than late-spring (0.22 ± 0.34‰). MN frequency observed among the five Italian towns showed a trend that follows broadly the levels of air pollution in Italy: the highest MN frequency was observed in Brescia during both seasons, the lowest in Lecce (winter) and Perugia (late-spring). CONCLUSIONS: To the best of our knowledge, the number of recruited children included in the analysis (n = 1046) is the highest compared to previous studies evaluating the frequency of MN in exfoliated buccal cells so far. MN frequency was associated with winter season and living in towns at various levels of air pollution, suggesting an important role of this exposure in determining early cytogenetic effects.
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