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Literature DB >> 2990907

Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene.

F W Bech, S T Jørgensen, B Diderichsen, O H Karlström.

Abstract

Escherichia coli relB mutants react to amino acid starvation by several abnormal responses, including accumulation of a translational inhibitor. We have isolated a relB-complementing plasmid from the Clarke and Carbon E. coli DNA library. From this plasmid we sequenced a 2140-bp segment which included the relB gene by the following two criteria: (i) it complements chromosomal relB mutations, (ii) the corresponding DNA segment cloned from chromosomal DNA of three relB mutants was defective in relB complementation. All three mutations fell within an open reading frame of 79 amino acids. A polypeptide of 9 kd compatible with this open reading frame was synthesized in maxicells and is in all probability the product of the relB gene. By nuclease S1 mapping we have determined the transcription start and stop of an 870 base transcript of the relB gene.

Entities: Chemical

Mesh：

Substances：
Bacterial Proteins

Year: 1985 PMID： 2990907 PMCID： PMC554300 DOI： 10.1002/j.1460-2075.1985.tb03739.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

33 in total

1. Regulation of bacterial ppGpp and pppGpp.

Authors: M Cashel
Journal: Annu Rev Microbiol Date: 1975 Impact factor: 15.500

2. Isolation of relaxed-control mutants of escherichia coli K-12 which are sensitive to glucose starvation.

Authors: R D Mosteller; S F Kwan
Journal: Biochem Biophys Res Commun Date: 1976-03-22 Impact factor: 3.575

3. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors: J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal: Proc Natl Acad Sci U S A Date: 1977-09 Impact factor: 11.205

4. Evidence that glucose starvation-sensitive mutants are altered in the relB locus.

Authors: R D Mosteller
Journal: J Bacteriol Date: 1978-02 Impact factor: 3.490

5. Genetics of the relB locus in Escherichia coli.

Authors: B Diderichsen; N P Fiil; R Lavallé
Journal: J Bacteriol Date: 1977-07 Impact factor: 3.490

6. On the production of deletions in the chromosome of Escherichia coli.

Authors: J A Spudich; V Horn; C Yanofsky
Journal: J Mol Biol Date: 1970-10-14 Impact factor: 5.469

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

8. [New mutants for regulation of RNA synthesis].

Authors: R Lavallé
Journal: Bull Soc Chim Biol (Paris) Date: 1965

9. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors: L Clarke; J Carbon
Journal: Cell Date: 1976-09 Impact factor: 41.582

10. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

Authors: J Shine; L Dalgarno
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

33 in total

Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene.

1. Regulation of bacterial ppGpp and pppGpp.

2. Isolation of relaxed-control mutants of escherichia coli K-12 which are sensitive to glucose starvation.

3. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

4. Evidence that glucose starvation-sensitive mutants are altered in the relB locus.

5. Genetics of the relB locus in Escherichia coli.

6. On the production of deletions in the chromosome of Escherichia coli.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. [New mutants for regulation of RNA synthesis].

9. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

10. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

1. Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes.

Review 2. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress.

3. RelE, a global inhibitor of translation, is activated during nutritional stress.

4. Induction of Escherichia coli chromosomal mazEF by stressful conditions causes an irreversible loss of viability.

5. A novel family of Escherichia coli toxin-antitoxin gene pairs.

6. The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome.

Review 7. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Review 8. Linkage map of Escherichia coli K-12, edition 8.

9. MazF-mediated cell death in Escherichia coli: a point of no return.

10. A differential effect of E. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation.