Literature DB >> 2989327

Rapid neutralization assay for human cytomegalovirus antibody.

M Zerbini, M Plazzi, M Musiani.   

Abstract

Human cytomegalovirus induces the appearance of immediate early antigens in infected cells 1 h after infection. This provided the basis for the development of a rapid neutralization assay for cytomegalovirus antibody which was able to yield results within a single day. Indirect immunofluorescence to visualize immediate early antigen-positive cells was applied to the rapid determination of cytomegalovirus-neutralizing antibody. The neutralization titers obtained with this assay on 92 serum samples were in accordance with the immune status as determined by enzyme-linked immunosorbent assay to cytomegalovirus-induced immediate early, early, and late antigens.

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Year:  1985        PMID： 2989327      PMCID： PMC271828          DOI： 10.1128/jcm.21.6.969-971.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS.

Authors:  G PLUMMER; M BENYESH-MELNICK
Journal:  Proc Soc Exp Biol Med       Date:  1964-10

2.  DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage.

Authors:  M F Stinski; E S Mocarski; D R Thomsen
Journal:  J Virol       Date:  1979-07       Impact factor: 5.103

3.  Antibodies against cytomegalovirus-induced early antigens (CMV-EA) in immunosuppressed renal-allograft recipients.

Authors:  T H The; H K Andersen; E S Spencer; G Klein
Journal:  Clin Exp Immunol       Date:  1977-06       Impact factor: 4.330

4.  Plaque reduction neutralization test for human cytomegalovirus based upon enhanced uptake of neutral red by virus-infected cells.

Authors:  N J Schmidt; J Dennis; E H Lennette
Journal:  J Clin Microbiol       Date:  1976-07       Impact factor: 5.948

5.  Immediate early antigens in human cytomegalovirus infected cells.

Authors:  S Michelson-Fiske; F Horodniceanu; J C Guillon
Journal:  Nature       Date:  1977-12-15       Impact factor: 49.962

6.  Immunoglobulin G to virus-specific early antigens in congenital, primary, and reactivated human cytomegalovirus infections.

Authors:  G Gerna; P M Cereda; E Cattaneo; G Achilli; M G Revello
Journal:  Infect Immun       Date:  1978-12       Impact factor: 3.441

7.  Productive and abortive replication of human cytomegalovirus at different environmental pH values.

Authors:  M Zerbini; M Musiani; C Carpi; E Falcieri
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

8.  Microneutalization of cytomegalovirus.

Authors:  H Stalder; A Ehrensberger
Journal:  J Infect Dis       Date:  1980-07       Impact factor: 5.226

9.  Three-day assay for human cytomegalovirus applicable to serum neutralization tests.

Authors:  J L Waner; J E Budnick
Journal:  Appl Microbiol       Date:  1973-01

10.  Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants.

Authors:  L Pereira; M Hoffman; D Gallo; N Cremer
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441
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  2 in total

1.  Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

Authors:  M Zerbini; M Musiani; G Gentilomi; M La Placa
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

2.  Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.

Authors:  S W Chou; K M Scott
Journal:  J Clin Microbiol       Date:  1988-03       Impact factor: 5.948
  2 in total

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