Literature DB >> 4346976

Three-day assay for human cytomegalovirus applicable to serum neutralization tests.

J L Waner, J E Budnick.   

Abstract

A fluorescing cell assay (FCA) technique utilizing the indirect fluorescent-antibody method to measure human cytomegalovirus (CMV)-infected cells has been applied to the rapid determination of CMV-neutralizing antibody. Human sera with complement fixation titers to CMV of 1/32 or greater and fluorescein-conjugated rabbit anti-human globulin are the primary and secondary reagents in the fluorescent-antibody test. FCA measured in 3 days the same number of infectious units measured by plaque assay in 2 weeks. FCA and plaque assay yielded identical neutralizing antibody titers to CMV in 20 human sera.

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Year:  1973        PMID： 4346976      PMCID： PMC380731          DOI： 10.1128/am.25.1.37-39.1973

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  6 in total

1.  A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS.

Authors:  G PLUMMER; M BENYESH-MELNICK
Journal:  Proc Soc Exp Biol Med       Date:  1964-10

2.  THE IMMUNOFLUORESCENT FOCUS TECHNIQUE IN STUDYING THE REPLICATION OF CYTOMEGALOVIRUS.

Authors:  F RAPP; L E RASMUSSEN; M BENYESH-MELNICK
Journal:  J Immunol       Date:  1963-11       Impact factor: 5.422

3.  Human cytomegalovirus. Assay by counting infected cells.

Authors:  C R GOODHEART; L B JAROSS
Journal:  Virology       Date:  1963-04       Impact factor: 3.616

4.  Serologic differentiation of viruses responsible for cytomegalic inclusion disease.

Authors:  T H WELLER; J B HANSHAW; D E SCOTT
Journal:  Virology       Date:  1960-09       Impact factor: 3.616

5.  Complement-requiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses.

Authors:  B J Graham; Y Minamishima; G R Dresman; H G Haines; M Benyesh-Melnick
Journal:  J Immunol       Date:  1971-12       Impact factor: 5.422

6.  Serologic differentiation of human cytomegalovirus strains using rabbit hyperimmune sera. Brief report.

Authors:  H K Andersen
Journal:  Arch Gesamte Virusforsch       Date:  1971
  6 in total
  4 in total

1.  Indirect fluorescent-antibody test for human cytomegalovirus infection in the absence of interfering immunoglobulin G receptors.

Authors:  N S Swack; F J Michalski; A Baumgarten; G D Hsiung
Journal:  Infect Immun       Date:  1977-05       Impact factor: 3.441

2.  Plaque reduction neutralization test for human cytomegalovirus based upon enhanced uptake of neutral red by virus-infected cells.

Authors:  N J Schmidt; J Dennis; E H Lennette
Journal:  J Clin Microbiol       Date:  1976-07       Impact factor: 5.948

3.  Blastogenic response of human lymphocytes to human cytomegalovirus.

Authors:  J L Waner; J E Budnick
Journal:  Clin Exp Immunol       Date:  1977-10       Impact factor: 4.330

4.  Rapid neutralization assay for human cytomegalovirus antibody.

Authors:  M Zerbini; M Plazzi; M Musiani
Journal:  J Clin Microbiol       Date:  1985-06       Impact factor: 5.948
  4 in total

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