Literature DB >> 2987868

Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37.

D E Akiyoshi, D A Regier, G Jen, M P Gordon.   

Abstract

The trans-zeatin secretion locus (tzs), from the nopaline Ti plasmid of Agrobacterium tumefaciens strain T37, was cloned and the nucleotide sequence determined. This gene is located in the virulence region of pTiT37. The tzs gene is responsible for the secretion of trans-zeatin into bacterial culture medium and in addition has the cytokinin biosynthetic activity, dimethylallylpyrophosphate:AMP dimethylallyltransferase. Sequence analysis showed an open reading frame of 729 nucleotides, capable of encoding a protein of 27,545 daltons. A single new labelled protein of 27,200 daltons was detected in Escherichia coli maxicells expressing the cloned tzs gene. Significant sequence homology was observed between the tzs and the published tmr sequence from pTiT37.

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Year:  1985        PMID： 2987868      PMCID： PMC341193          DOI： 10.1093/nar/13.8.2773

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  45 in total

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Authors:  G Ooms; P J Hooykaas; G Moolenaar; R A Schilperoort
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  28 in total

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10.  Reexamining the role of the accessory plasmid pAtC58 in the virulence of Agrobacterium tumefaciens strain C58.

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