Literature DB >> 24213790

Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity.

M F van Wordragen1, J de Jong, H B Huitema, H J Dons.   

Abstract

To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few genotype/strain combinations resulted in significant tumour formation. Especially 0-type strains were highly efficient. An 0-type strain was used to transfer genes for neomycine phosphotransferase (NPT II) and ß-glucuronidase (GUS) to a susceptible cultivar. Transfer of the GUS gene was confirmed by using the Polymerase Chain Reaction (PCR).

Entities:  

Year:  1991        PMID： 24213790     DOI： 10.1007/BF00232106

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  12 in total

1.  Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens.

Authors:  J Puonti-Kaerlas; P Stabel; T Eriksson
Journal:  Plant Cell Rep       Date:  1989-06       Impact factor: 4.570

2.  Dot assay for neomycin phosphotransferase activity in crude cell extracts.

Authors:  S G Platt; N S Yang
Journal:  Anal Biochem       Date:  1987-05-01       Impact factor: 3.365

3.  Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37.

Authors:  D E Akiyoshi; D A Regier; G Jen; M P Gordon
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

4.  Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281.

Authors:  S G Jin; T Komari; M P Gordon; E W Nester
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

5.  Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.

Authors:  K Mullis; F Faloona; S Scharf; R Saiki; G Horn; H Erlich
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1986

6.  Fingerprints of Agrobacterium Ti plasmids.

Authors:  D Sciaky; A L Montoya; M D Chilton
Journal:  Plasmid       Date:  1978-02       Impact factor: 3.466

7.  The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.

Authors:  E E Hood; G L Helmer; R T Fraley; M D Chilton
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

8.  Physical and functional map of supervirulent Agrobacterium tumefaciens tumor-inducing plasmid pTiBo542.

Authors:  T Komari; W Halperin; E W Nester
Journal:  J Bacteriol       Date:  1986-04       Impact factor: 3.490

9.  The chalcone synthase multigene family of Petunia hybrida (V30): differential, light-regulated expression during flower development and UV light induction.

Authors:  R E Koes; C E Spelt; J N Mol
Journal:  Plant Mol Biol       Date:  1989-02       Impact factor: 4.076

10.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
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  5 in total

1.  Insect-resistant chrysanthemum calluses by introduction of a Bacillus thuringiensis crystal protein gene.

Authors:  M F van Wordragen; G Honée; H J Dons
Journal:  Transgenic Res       Date:  1993-05       Impact factor: 2.788

2.  Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens.

Authors:  C N Liu; X Q Li; S B Gelvin
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076

3.  Crown gall transformation of lentil (Lens culinaris Medik.) with virulent strains of Agrobacterium tumefaciens.

Authors:  T D Warkentin; A McHughen
Journal:  Plant Cell Rep       Date:  1991-12       Impact factor: 4.570

4.  Biolistic transformation of chrysanthemum with the nucleocapsid gene of tomato spotted wilt virus.

Authors:  L M Yepes; V Mittak; S Z Pang; C Gonsalves; J L Slightom; D Gonsalves
Journal:  Plant Cell Rep       Date:  1995-08       Impact factor: 4.570

5.  Forecasting and optimizing Agrobacterium-mediated genetic transformation via ensemble model- fruit fly optimization algorithm: A data mining approach using chrysanthemum databases.

Authors:  Mohsen Hesami; Milad Alizadeh; Roohangiz Naderi; Masoud Tohidfar
Journal:  PLoS One       Date:  2020-09-30       Impact factor: 3.240
  5 in total

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