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Literature DB >> 2985438

Insulin release independent of a rise in cytosolic free Ca2+ by forskolin and phorbol ester.

Abstract

The role of cytosolic free Ca2+ in insulin release was evaluated using isolated rat pancreatic islets permeabilized with digitonin and incubated in Ca-EGTA buffers to fix free Ca2+ concentration at arbitrary levels. Ca2+ induced insulin release in a concentration-dependent manner with the threshold being between 0.1 and 1 microM. The hormone release was increased by forskolin and 12-O-tetradecanoyl phorbol-13-acetate (TPA), a potent activator of adenylate cyclase and that of protein kinase C, respectively. The findings suggest that activation of both protein kinase A and protein kinase C modulate insulin release without a concomitant increase in cytosolic free Ca2+.

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Year: 1985 PMID： 2985438 DOI： 10.1016/0014-5793(85)80825-5

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

27 in total

1. Distinct mechanisms for two amplification systems of insulin release.

Authors: J C Henquin; M Bozem; W Schmeer; M Nenquin
Journal: Biochem J Date: 1987-09-01 Impact factor: 3.857

2. Somatostatin inhibition of Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells.

Authors: S Ullrich; M Prentki; C B Wollheim
Journal: Biochem J Date: 1990-08-15 Impact factor: 3.857

3. Effects of Bordetella pertussis toxin on catecholamine inhibition of insulin release from intact and electrically permeabilized rat islets.

Authors: S J Persaud; P M Jones; S L Howell
Journal: Biochem J Date: 1989-03-15 Impact factor: 3.857

4. Different insulin-secretory responses to calcium-channel blockers in islets of lean and obese (ob/ob) mice.

Authors: M A Black; L A Fournier; H M Heick; N Bégin-Heick
Journal: Biochem J Date: 1988-01-15 Impact factor: 3.857

5. Phorbol-ester-induced down-regulation of protein kinase C in mouse pancreatic islets. Potentiation of phase 1 and inhibition of phase 2 of glucose-induced insulin secretion.

Authors: P Thams; K Capito; C J Hedeskov; H Kofod
Journal: Biochem J Date: 1990-02-01 Impact factor: 3.857

10. Ca2(+)-induced insulin secretion from electrically permeabilized islets. Loss of the Ca2(+)-induced secretory response is accompanied by loss of Ca2(+)-induced protein phosphorylation.

Authors: P M Jones; S J Persaud; S L Howell
Journal: Biochem J Date: 1992-08-01 Impact factor: 3.857

Insulin release independent of a rise in cytosolic free Ca2+ by forskolin and phorbol ester.

1. Distinct mechanisms for two amplification systems of insulin release.

2. Somatostatin inhibition of Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells.

3. Effects of Bordetella pertussis toxin on catecholamine inhibition of insulin release from intact and electrically permeabilized rat islets.

4. Different insulin-secretory responses to calcium-channel blockers in islets of lean and obese (ob/ob) mice.

5. Phorbol-ester-induced down-regulation of protein kinase C in mouse pancreatic islets. Potentiation of phase 1 and inhibition of phase 2 of glucose-induced insulin secretion.

6. Roles of intracellular Ca2+ receptors in the pancreatic beta-cell in insulin secretion.

7. Multiple exocytotic pathways in pancreatic beta cells.

8. Effect of xanthohumol on melanogenesis in B16 melanoma cells.

9. Enhancement of glucagon secretion from isolated rat islets of Langerhans by phorbol 12-myristate 13-acetate.

10. Ca2(+)-induced insulin secretion from electrically permeabilized islets. Loss of the Ca2(+)-induced secretory response is accompanied by loss of Ca2(+)-induced protein phosphorylation.