Literature DB >> 2981329

Middle tumor antigen of polyomavirus transformation-defective mutant NG59 is associated with pp60c-src.

J B Bolen, M A Israel.   

Abstract

We have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant NG59 under gentle conditions that avoid ionic detergents results in detectable NG59-encoded middle tumor antigen (MTAg) associated with pp60c-src. This MTAg-pp60c-src complex could be immunoprecipitated from NG59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against MTAg. Immune complex kinase assays revealed that, whereas the pp60c-src associated with NG59 MTAg possessed tyrosyl kinase activity, the NG59 MTAg in this complex was not phosphorylated in these in vitro reactions. These results demonstrate that the point insertion mutation found in this transformation-deficient strain of polyomavirus encodes MTAg molecules capable of associating with pp60c-src and defines a limited region within MTAg which appears to be critical for stable MTAg-pp60c-src interactions.

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Year:  1985        PMID： 2981329      PMCID： PMC254986     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  35 in total

1.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

2.  Protein kinase activity associated with polyoma virus middle T antigen in vitro.

Authors:  A E Smith; R Smith; B Griffin; M Fried
Journal:  Cell       Date:  1979-12       Impact factor: 41.582

3.  Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.

Authors:  B S Schaffhausen; J E Silver; T L Benjamin
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

4.  Characterization of T antigens in polyoma-infected and transformed cells.

Authors:  M A Hutchinson; T Hunter; W Eckhart
Journal:  Cell       Date:  1978-09       Impact factor: 41.582

5.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

6.  Identification of DNA sequence changes leading to loss of transforming ability in polyoma virus.

Authors:  G G Carmichael; T L Benjamin
Journal:  J Biol Chem       Date:  1980-01-10       Impact factor: 5.157

7.  Heat-labile serum factor required for immunofluorescence of polyoma tumor antigens.

Authors:  K K Takemoto; R A Malmgren; K Habel
Journal:  Science       Date:  1966-09-02       Impact factor: 47.728

8.  Localization of gene functions in polyoma virus DNA.

Authors:  J Feunteun; L Sompayrac; M Fluck; T Benjamin
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

9.  Virus-specific proteins in the plasma membrane of cells lytically infected or transformed by pol-oma virus.

Authors:  Y Ito; J R Brocklehurst; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

10.  Polyoma virus transforming protein associates with the product of the c-src cellular gene.

Authors:  S A Courtneidge; A E Smith
Journal:  Nature       Date:  1983 Jun 2-8       Impact factor: 49.962
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  29 in total

1.  Functional asymmetry of the regions juxtaposed to the membrane-binding sequence of polyomavirus middle T antigen.

Authors:  J Dahl; U Thathamangalam; R Freund; T L Benjamin
Journal:  Mol Cell Biol       Date:  1992-11       Impact factor: 4.272

2.  Interactions between polyomavirus medium T antigen and three cellular proteins of 88, 61, and 37 kilodaltons.

Authors:  T Grussenmeyer; A Carbone-Wiley; K H Scheidtmann; G Walter
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

3.  Dephosphorylation or antibody binding to the carboxy terminus stimulates pp60c-src.

Authors:  J A Cooper; C S King
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

4.  Structural and functional modification of pp60c-src associated with polyoma middle tumor antigen from infected or transformed cells.

Authors:  C A Cartwright; M A Hutchinson; W Eckhart
Journal:  Mol Cell Biol       Date:  1985-10       Impact factor: 4.272

5.  12-O-tetradecanoylphorbol-13-acetate stimulates phosphorylation of the 58,000-Mr form of polyomavirus middle T antigen in vivo: implications for a possible role of protein kinase C in middle T function.

Authors:  J T Matthews; T L Benjamin
Journal:  J Virol       Date:  1986-05       Impact factor: 5.103

6.  The amino terminus of polyomavirus middle T antigen is required for transformation.

Authors:  D N Cook; J A Hassell
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

7.  Polyomavirus small t antigen: overproduction in bacteria, purification, and utilization for monoclonal and polyclonal antibody production.

Authors:  D C Pallas; C Schley; M Mahoney; E Harlow; B S Schaffhausen; T M Roberts
Journal:  J Virol       Date:  1986-12       Impact factor: 5.103

8.  Medium tumor antigen of polyomavirus transformation-defective mutant NG59 is associated with 73-kilodalton heat shock protein.

Authors:  G Walter; A Carbone; W J Welch
Journal:  J Virol       Date:  1987-02       Impact factor: 5.103

9.  Purified polyoma virus medium T antigen has tyrosine-specific protein kinase activity but no significant phosphatidylinositol kinase activity.

Authors:  W Koch; A Carbone; G Walter
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

10.  Novel monoclonal antibodies that differentiate between the binding of pp60c-src or protein phosphatase 2A by polyomavirus middle T antigen.

Authors:  S M Dilworth; V P Horner
Journal:  J Virol       Date:  1993-04       Impact factor: 5.103
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