Michal Kramer1,2, Murat Hasanreisoglu3, Shirel Weiss2,4, Deniz Kumova3, Michal Schaap-Fogler1, Sezen Guntekin-Ergun5, Sengul Ozdek3, Gokhan Gurelik3, Mehmet Ali Ergün5, Nitza Goldenberg-Cohen6, Yoram Cohen2,7. 1. a Department of Ophthalmology, Rabin Medical Center , Petach Tikva , Israel. 2. b Sackler Faculty of Medicine, Tel Aviv University , Tel Aviv , Israel. 3. c Department of Ophthalmology, Gazi University, School of Medicine , Ankara , Turkey. 4. d The Krieger Eye Research Laboratory, Felsenstein Medical Research Center, Rabin Medical Center , Petach Tikva , Israel. 5. e Department of Medical Genetics, Gazi University, School of Medicine , Ankara , Turkey. 6. f Department of Ophthalmology, Bnei-Zion Medical Center, Rappaport School of Medicine, Technion , Haifa , Israel. 7. g Department of Obstetrics and Gynecology, Sheba Medical Center , Tel Hashomer , Israel.
Abstract
Purpose: To test the frequency of single-nucleotide polymorphisms in the IL-10, IL23R-IL12RB2 genes in patients with Behcet's uveitis. Methods: Blood samples were collected from 89 Israeli and Turkish patients, and from healthy control subjects of different origins. Genomic DNA was extracted from peripheral blood leukocytes and genotyped. Results: The risk allele, A, in rs1800871, of IL-10 gene was highly prevalent in Behcet's uveitis and healthy control samples alike; highest among the Turkish groups. Prevalence of G allele, in rs1495965, in the IL23R-IL12RB2 gene was high in Behcet's uveitis patients, and among healthy Turkish and Israelis of Middle Eastern origin, while lower among the other Israeli control group (77.9%, 78.9%, 27.8%, respectively, P < 0.001). Conclusion: Our findings highlight the differences between populations and may account for the increased prevalence of the disease among Turkish and Israelis of Middle Eastern origin. Further studies are required to map other healthy and affected populations.
Purpose: To test the frequency of single-nucleotide polymorphisms in the IL-10, IL23R-IL12RB2 genes in patients with Behcet's uveitis. Methods: Blood samples were collected from 89 Israeli and Turkish patients, and from healthy control subjects of different origins. Genomic DNA was extracted from peripheral blood leukocytes and genotyped. Results: The risk allele, A, in rs1800871, of IL-10 gene was highly prevalent in Behcet's uveitis and healthy control samples alike; highest among the Turkish groups. Prevalence of G allele, in rs1495965, in the IL23R-IL12RB2 gene was high in Behcet's uveitispatients, and among healthy Turkish and Israelis of Middle Eastern origin, while lower among the other Israeli control group (77.9%, 78.9%, 27.8%, respectively, P < 0.001). Conclusion: Our findings highlight the differences between populations and may account for the increased prevalence of the disease among Turkish and Israelis of Middle Eastern origin. Further studies are required to map other healthy and affected populations.