| Literature DB >> 29786090 |
Keith R Anderson1, Maximilian Haeussler2, Colin Watanabe3, Vasantharajan Janakiraman1, Jessica Lund1, Zora Modrusan1, Jeremy Stinson1, Qixin Bei1, Andrew Buechler1, Charles Yu1, Sobha R Thamminana1, Lucinda Tam1, Michael-Anne Sowick4, Tuija Alcantar4, Natasha O'Neil4, Jinjie Li4, Linda Ta4, Lisa Lima4, Merone Roose-Girma1, Xin Rairdan4, Steffen Durinck1, Søren Warming5.
Abstract
Despite widespread use of CRISPR, comprehensive data on the frequency and impact of Cas9-mediated off-targets in modified rodents are limited. Here we present deep-sequencing data from 81 genome-editing projects on mouse and rat genomes at 1,423 predicted off-target sites, 32 of which were confirmed, and show that high-fidelity Cas9 versions reduced off-target mutation rates in vivo. Using whole-genome sequencing data from ten mouse embryos, treated with a single guide RNA (sgRNA), and from their genetic parents, we found 43 off-targets, 30 of which were predicted by an adapted version of GUIDE-seq.Entities:
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Year: 2018 PMID： 29786090 PMCID： PMC6558654 DOI： 10.1038/s41592-018-0011-5
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547