Genwen Hu1, Wen Liang2, Mingxiang Wu1, Caiyong Lai3, Yingjie Mei4, Yufa Li5, Jianmin Xu6, Liangping Luo7, Xianyue Quan8. 1. Department of Radiology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, No.1017, Dongmen Road, Shenzhen, 518020, China. 2. Department of Radiology, Zhujiang Hospital, Southern Medical University, No.253, Gongye Road, Guangzhou, 510282, China. 3. Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China. 4. MR Clinical Science, Philips Healthcare, GuangZhou, China. 5. Department of Pathology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China. 6. Department of Radiology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, No.1017, Dongmen Road, Shenzhen, 518020, China. Electronic address: 13600163204@163.com. 7. Medical Imaging Center, The First Affiliated Hospital of Jinan University, No.613, Huangpu Road, Guangzhou, 510630, China. Electronic address: tluolp@jnu.edu.cn. 8. Department of Radiology, Zhujiang Hospital, Southern Medical University, No.253, Gongye Road, Guangzhou, 510282, China. Electronic address: quanxianyue2014@163.com.
Abstract
RATIONALE AND OBJECTIVES: The aim of this study was to investigate the potential of magnetic resonance imaging (MRI) T1 mapping and T1 relaxation time in the rotating frame (T1rho) for assessment of renal fibrosis in a rat model of unilateral ureteral obstruction (UUO). MATERIALS AND METHODS: UUO was created in 36 rats. Six rats were scanned at each of the six time points (on days 0, 1, 3, 5, 10, and 15 after UUO). The contralateral kidneys were examined as controls. Hematoxylin-eosin, Masson's trichrome, and alpha-smooth muscle actin (α-SMA) antibody staining assays were performed. MRI data obtained with a 3.0T scanner were analyzed with α-SMA expression and Masson's staining. RESULTS: The T1 relaxation times and T1rho values increased, and the mean apparent diffusion coefficient (ADC) values decreased with time after UUO. Simple regression analysis indicated that the mean ADCs, T1 relaxation times, and T1rho values had strong correlations with the α-SMA expression levels (R2 = 0.34, R2 = 0.66, R2 = 0.71, respectively; P< .001) and positive Masson's staining (R2 = 0.38, R2 = 0.67, R2 = 0.65, respectively; P< .001). CONCLUSIONS: The T1 mapping and T1rho parameters had better correlations with α-SMA expression and Masson's staining than ADC values.
RATIONALE AND OBJECTIVES: The aim of this study was to investigate the potential of magnetic resonance imaging (MRI) T1 mapping and T1 relaxation time in the rotating frame (T1rho) for assessment of renal fibrosis in a rat model of unilateral ureteral obstruction (UUO). MATERIALS AND METHODS: UUO was created in 36 rats. Six rats were scanned at each of the six time points (on days 0, 1, 3, 5, 10, and 15 after UUO). The contralateral kidneys were examined as controls. Hematoxylin-eosin, Masson's trichrome, and alpha-smooth muscle actin (α-SMA) antibody staining assays were performed. MRI data obtained with a 3.0T scanner were analyzed with α-SMA expression and Masson's staining. RESULTS: The T1 relaxation times and T1rho values increased, and the mean apparent diffusion coefficient (ADC) values decreased with time after UUO. Simple regression analysis indicated that the mean ADCs, T1 relaxation times, and T1rho values had strong correlations with the α-SMA expression levels (R2 = 0.34, R2 = 0.66, R2 = 0.71, respectively; P< .001) and positive Masson's staining (R2 = 0.38, R2 = 0.67, R2 = 0.65, respectively; P< .001). CONCLUSIONS: The T1 mapping and T1rho parameters had better correlations with α-SMA expression and Masson's staining than ADC values.
Authors: Stefanie J Hectors; Philippe Garteiser; Sabrina Doblas; Gwenaël Pagé; Bernard E Van Beers; John C Waterton; Octavia Bane Journal: Methods Mol Biol Date: 2021
Authors: Feng Wang; Daniel C Colvin; Suwan Wang; Hua Li; Zhongliang Zu; Raymond C Harris; Ming-Zhi Zhang; John C Gore Journal: Magn Reson Med Date: 2020-03-06 Impact factor: 4.668