Literature DB >> 2961651

A family of cosmid vectors with the multi-copy R6K replication origin.

E Ehrich1, A Craig, A Poustka, A M Frischauf, H Lehrach.   

Abstract

A family of cosmid vectors was constructed which contain replication origins (ori) derived from the multicopy plasmid R6K, a kanamycin resistance gene and two cos sites, permitting efficient library construction. Additional features of later constructs are (i) the presence of NotI sites flanking the site of insertion to allow intact excision of inserts, (ii) the facility for selective cloning of the ends of inserts for rapid chromosome walking, and (iii) the use of a mutated R6K ori leading to an increased copy number.

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Year:  1987        PMID： 2961651     DOI： 10.1016/0378-1119(87)90126-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  10 in total

1.  The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP.

Authors:  Z Kozmik; S Wang; P Dörfler; B Adams; M Busslinger
Journal:  Mol Cell Biol       Date:  1992-06       Impact factor: 4.272

2.  The human embryonic myosin alkali light chain gene: use of alternative promoters and 3' non-coding regions.

Authors:  M Rotter; K Zimmerman; A Poustka; N Soussi-Yanicostas; A Starzinski-Powitz
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

3.  Multiple mechanisms of N-phosphonacetyl-L-aspartate resistance in human cell lines: carbamyl-P synthetase/aspartate transcarbamylase/dihydro-orotase gene amplification is frequent only when chromosome 2 is rearranged.

Authors:  K A Smith; O B Chernova; R P Groves; M B Stark; J L Martínez; J N Davidson; J M Trent; T E Patterson; A Agarwal; P Duncan; M L Agarwal; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-04       Impact factor: 11.205

4.  c2X75, a derivative of the cosmid vector c2XB.

Authors:  D A Campbell
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

5.  Genomic structure of the human CD53 gene.

Authors:  V Korínek; V Horejsí
Journal:  Immunogenetics       Date:  1993       Impact factor: 2.846

6.  Cosmid cloning and restriction endonuclease mapping of the herpesvirus of turkeys (HVT) genome.

Authors:  W Lindenmaier; H J Bauer
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

7.  Alternative splicing of Pax-8 gene transcripts is developmentally regulated and generates isoforms with different transactivation properties.

Authors:  Z Kozmik; R Kurzbauer; P Dörfler; M Busslinger
Journal:  Mol Cell Biol       Date:  1993-10       Impact factor: 4.272

8.  Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos.

Authors:  P Chavrier; U Janssen-Timmen; M G Mattéi; M Zerial; R Bravo; P Charnay
Journal:  Mol Cell Biol       Date:  1989-02       Impact factor: 4.272

9.  A new method for specific cleavage of megabase-size chromosomal DNA by lambda-terminase.

Authors:  Y Wang; R Wu
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

10.  The murine Spi-2 proteinase inhibitor locus: a multigene family with a hypervariable reactive site domain.

Authors:  J D Inglis; R E Hill
Journal:  EMBO J       Date:  1991-02       Impact factor: 11.598
  10 in total

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