Nitin Kumar1, Varsha A Singh1, Vikas Beniwal2, Shinu Pottathil1. 1. M M Institute of Medical Sciences and Research, Mullana, Ambala, Haryana, India. 2. Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India.
Abstract
BACKGROUND: The aim of this study was to evaluate the modified Carba NP test to differentiate KPC (Klebsiella pneumoniae carbapenemase)- and MBL (metallo-β-lactamase)-producing Klebsiella species. METHODS: A total of 508 non-duplicate clinical isolates of Klebsiella spp. were processed by modified Carba NP and combined disc tests which were further confirmed by conventional polymerase chain reaction (PCR), a gold standard method for statistical analysis. RESULTS: Modified Carba NP test demonstrated 91.7% sensitivity, 100% specificity, 100% positive predictive value (PPV) and 99.8% negative predictive value (NPV) for KPC and 96.7%, 100%, 100%, and 99.5% for MBL detection, respectively. CONCLUSION: The performance of modified Carba NP test was significantly better than combined disc test, fulfilling the requirement of simple and rapid test for clinical applications.
BACKGROUND: The aim of this study was to evaluate the modified Carba NP test to differentiate KPC (Klebsiella pneumoniae carbapenemase)- and MBL (metallo-β-lactamase)-producing Klebsiella species. METHODS: A total of 508 non-duplicate clinical isolates of Klebsiella spp. were processed by modified Carba NP and combined disc tests which were further confirmed by conventional polymerase chain reaction (PCR), a gold standard method for statistical analysis. RESULTS: Modified Carba NP test demonstrated 91.7% sensitivity, 100% specificity, 100% positive predictive value (PPV) and 99.8% negative predictive value (NPV) for KPC and 96.7%, 100%, 100%, and 99.5% for MBL detection, respectively. CONCLUSION: The performance of modified Carba NP test was significantly better than combined disc test, fulfilling the requirement of simple and rapid test for clinical applications.
Authors: K van Dijk; G M Voets; J Scharringa; S Voskuil; A C Fluit; W C Rottier; M A Leverstein-Van Hall; J W T Cohen Stuart Journal: Clin Microbiol Infect Date: 2013-08-09 Impact factor: 8.067
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Authors: Pottathil Shinu; Abdulaziz K Al Mouslem; Anroop B Nair; Katharigatta N Venugopala; Mahesh Attimarad; Varsha A Singh; Sreeharsha Nagaraja; Ghallab Alotaibi; Pran Kishore Deb Journal: Pharmaceuticals (Basel) Date: 2022-03-28