Literature DB >> 2960248

Determination of trace amounts of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography.

C V Patel1, K P Gopinathan.   

Abstract

A high-performance liquid chromatographic method to separate five major bases (cytosine, thymine, guanine, adenine, and uracil) and three minor methylated bases (5-methylcytosine, N6-methyladenine, and 7-methylguanine) has been developed using a volatile mobile phase under isocratic conditions. It is extended to quantitate 5-methylcytosine in trace amounts (1 in 20,000 cytosine residues). The suitability of the method has been verified by estimating 5-methylcytosine in DNAs of phi X174 and pBR322. The method has been applied to quantitate the extent of cytosine methylation in DNA of larval silk glands of Bombyx mori. Our results confirm that the pupal DNA of Drosophila melanogaster does not contain detectable amounts of 5-methylcytosine.

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Year:  1987        PMID： 2960248     DOI： 10.1016/0003-2697(87)90381-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  19 in total

1.  Genomic imprinting and position-effect variegation in Drosophila melanogaster.

Authors:  V K Lloyd; D A Sinclair; T A Grigliatti
Journal:  Genetics       Date:  1999-04       Impact factor: 4.562

Review 2.  Identifying 5-methylcytosine and related modifications in DNA genomes.

Authors:  T Rein; M L DePamphilis; H Zorbas
Journal:  Nucleic Acids Res       Date:  1998-05-15       Impact factor: 16.971

Review 3.  Epigenetics with special reference to the human X chromosome inactivation and the enigma of Drosophila DNA methylation.

Authors:  Deepti Deobagkar
Journal:  J Genet       Date:  2018-06       Impact factor: 1.166

4.  Identification of a silencing element in the human 15q11-q13 imprinting center by using transgenic Drosophila.

Authors:  F Lyko; K Buiting; B Horsthemke; R Paro
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-17       Impact factor: 11.205

5.  Single base-resolution methylome of the silkworm reveals a sparse epigenomic map.

Authors:  Hui Xiang; Jingde Zhu; Quan Chen; Fangyin Dai; Xin Li; Muwang Li; Hongyu Zhang; Guojie Zhang; Dong Li; Yang Dong; Li Zhao; Ying Lin; Daojun Cheng; Jian Yu; Jinfeng Sun; Xiaoyu Zhou; Kelong Ma; Yinghua He; Yangxing Zhao; Shicheng Guo; Mingzhi Ye; Guangwu Guo; Yingrui Li; Ruiqiang Li; Xiuqing Zhang; Lijia Ma; Karsten Kristiansen; Qiuhong Guo; Jianhao Jiang; Stephan Beck; Qingyou Xia; Wen Wang; Jun Wang
Journal:  Nat Biotechnol       Date:  2010-05-02       Impact factor: 54.908

Review 6.  The dynamic nature of DNA methylation: a role in response to social and seasonal variation.

Authors:  Sebastian Alvarado; Russell D Fernald; Kenneth B Storey; Moshe Szyf
Journal:  Integr Comp Biol       Date:  2014-05-10       Impact factor: 3.326

Review 7.  DNA methylation and cell memory.

Authors:  A D Riggs
Journal:  Cell Biophys       Date:  1989 Aug-Oct

8.  Methyl-CpG-binding protein MeCP2 represses Sp1-activated transcription of the human leukosialin gene when the promoter is methylated.

Authors:  S Kudo
Journal:  Mol Cell Biol       Date:  1998-09       Impact factor: 4.272

Review 9.  DNA methylation and methyl-CpG binding proteins: developmental requirements and function.

Authors:  Ozren Bogdanović; Gert Jan C Veenstra
Journal:  Chromosoma       Date:  2009-06-09       Impact factor: 4.316

10.  The genome of the stick insect Medauroidea extradentata is strongly methylated within genes and repetitive DNA.

Authors:  Veiko Krauss; Carina Eisenhardt; Tina Unger
Journal:  PLoS One       Date:  2009-09-29       Impact factor: 3.240
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