Adem Altunkol1, Murat Savaş2, Fuat Dilmeç3, Mehmet Mazhar Utanğaç4, Deniz Abat1, Kemal Gümüş5, İsmail Karlıdağ6, Ercan Yeni7. 1. Department of Urology, University of Health Sciences, Adana Numune Training and Research Hospital, Adana, Turkey. 2. Department of Urology, University of Health Sciences, Antalya Training and Research Hospital, Antalya, Turkey. 3. Department of Medical Biology and Genetics, Harran University School of Medicine, Şanlıurfa, Turkey. 4. Department of Childhood Urology, Uludağ University School of Medicine, Bursa, Turkey. 5. Clinic of Urology, Ministry of Health, Balıklıgöl State Hospital, Şanlıurfa, Turkey. 6. Department of Urology, University of Health Sciences, Mehmet Akif İnan Training and Research Hospital, Şanlıurfa, Turkey. 7. Department of Urology, Harran University School of Medicine, Şanlıurfa, Turkey.
Abstract
OBJECTIVE: Understanding genetic polymorphisms might facilitate the analysis of differences between individuals in their susceptibility to developing cancers as a result of environmental carcinogens. Skin, lung, colon and bladder cancers emerge from biological defects in GSTM1, GSTT1 and GSTP1 gene expressions. In this study, we aimed to investigate whether there was an association between CYP1A1 and GSTP1 gene polymorphisms and bladder cancer in a Turkish population. MATERIAL AND METHODS: Blood samples were collected from 120 individuals (60 patients with bladder cancer and 60 healthy individuals), and their DNAs were isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) method was used to detect the frequencies of CYP1A1 NM_000499.3: c.*1189T > C and GSTP1 NM_000852.3: c.313A > G polymorphisms in bladder cancer patients. RESULTS: The frequency of the CYP1A1: c.*1189 TC genotype and C allele were significantly different between bladder cancer patients and healthy individuals (p=0.001 and p=0.005, respectively). However, there was no significant difference for the GSTP1: c.313 AG genotype or G allele between both study groups (p=0.699 and p=0.360, respectively). CONCLUSION: A polymorphic site of the CYP1A1 gene might be involved in the development of bladder cancer. However, the investigated GSTP1 polymorphic site did not represent an important risk factor for the development of bladder cancer in a Turkish population.
OBJECTIVE: Understanding genetic polymorphisms might facilitate the analysis of differences between individuals in their susceptibility to developing cancers as a result of environmental carcinogens. Skin, lung, colon and bladder cancers emerge from biological defects in GSTM1, GSTT1 and GSTP1 gene expressions. In this study, we aimed to investigate whether there was an association between CYP1A1 and GSTP1 gene polymorphisms and bladder cancer in a Turkish population. MATERIAL AND METHODS: Blood samples were collected from 120 individuals (60 patients with bladder cancer and 60 healthy individuals), and their DNAs were isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) method was used to detect the frequencies of CYP1A1 NM_000499.3: c.*1189T > C and GSTP1 NM_000852.3: c.313A > G polymorphisms in bladder cancer patients. RESULTS: The frequency of the CYP1A1: c.*1189 TC genotype and C allele were significantly different between bladder cancer patients and healthy individuals (p=0.001 and p=0.005, respectively). However, there was no significant difference for the GSTP1: c.313 AG genotype or G allele between both study groups (p=0.699 and p=0.360, respectively). CONCLUSION: A polymorphic site of the CYP1A1 gene might be involved in the development of bladder cancer. However, the investigated GSTP1 polymorphic site did not represent an important risk factor for the development of bladder cancer in a Turkish population.
Authors: Anthony A Fryer; Helen M Ramsay; Tracy J Lovatt; Peter W Jones; Carmel M Hawley; David L Nicol; Richard C Strange; Paul N Harden Journal: Carcinogenesis Date: 2004-09-30 Impact factor: 4.944
Authors: Alessio Naccarati; Pavel Soucek; Rudolf Stetina; Vincent Haufroid; Rajiv Kumar; Ludmila Vodickova; Katerina Trtkova; Maria Dusinska; Kari Hemminki; Pavel Vodicka Journal: Mutat Res Date: 2005-07-25 Impact factor: 2.433