Literature DB >> 295042

Segregation of rat chromosomes in somatic cell hybrids between rat cells and HT 1080 human fibrosarcoma cells.

G G D'Ancona, C M Croce.   

Abstract

We produced somatic cell hybrids between HT 1080-6TG human fibrosarcoma cells and either rat white blood cells (WBC) or cells directly derived from rat spleen. Karyologic and isozyme analyses of hybrid cells indicated that they preferentially lose rat chromosomes. Hypoxanthine-aminopterine thymidine-selected hybrid clones expressing rat hypoxanthine phosphoribosyltransferase (HPRT), glucose-6-phosphate dehydrogenase (G6PD), and phosphoglycerate kinase (PGK) and containing the rat X chromosome were counterselected in a medium containing 30 micrograms/ml of 6-thioguanine. Concordant loss of the rat X chromosome and of the expression of rat HPRT and G6PD was observed in the hybrid clones.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 295042     DOI： 10.1007/bf00289444

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  20 in total

1.  Synteny of the genes for thymidine kinase and galactokinase in the mouse and their assignment to mouse chromosome 11.

Authors:  P McBreen; K G Orkwiszewski; C J Chern; W J Mellman; C M Croce
Journal:  Cytogenet Cell Genet       Date:  1977

Review 2.  A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels.

Authors:  E A Nichols; F H Ruddle
Journal:  J Histochem Cytochem       Date:  1973-12       Impact factor: 2.479

3.  Unidirectional loss of human chromosomes in rat-human hybrids.

Authors:  C M Croce; I Kieba; H Koprowski
Journal:  Exp Cell Res       Date:  1973-06       Impact factor: 3.905

4.  Isozymes and genetic control of NADP-malate dehydrogenase in mice.

Authors:  N S Henderson
Journal:  Arch Biochem Biophys       Date:  1966-10       Impact factor: 4.013

5.  Polymorphism and linkage for mannosephosphate isomerase in Mus musculus.

Authors:  E A Nichols; V M Chapman; F H Ruddle
Journal:  Biochem Genet       Date:  1973-01       Impact factor: 1.890

6.  Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man.

Authors:  T R Chen; F A McMorris; R Creagan; F Ricciuti; J Tischfield; F Ruddle
Journal:  Am J Hum Genet       Date:  1973-03       Impact factor: 11.025

7.  Assignment of the gene for lactic dehydrogenase A to mouse chromosome 7 using mouse-human hybrids.

Authors:  D O'Brien; A Linnenbach; C M Croce
Journal:  Cytogenet Cell Genet       Date:  1978

8.  Loss of human genetic markers in man--Chinese hamster somatic cell hybrids.

Authors:  A Westerveld; R P Visser; P Meera Khan; D Bootsma
Journal:  Nat New Biol       Date:  1971-11-03

9.  Human regulatory gene for inducible tyrosine aminotransferase in rat-human hybrids.

Authors:  C M Croce; G Litwack; H Koprowski
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

10.  Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes.

Authors:  M C Weiss; H Green
Journal:  Proc Natl Acad Sci U S A       Date:  1967-09       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.