BACKGROUND/AIMS: Diabetes mellitus (DM) has been demonstrated to have a strong association with heart failure. Conventional echocardiographic analysis cannot sensitively monitor cardiac dysfunction in type I diabetic Akita hearts, but the phenotype of heart failure is observed in molecular levels during the early stages. METHODS: Male Akita (Ins2WT/C96Y) mice were monitored with echocardiographic imaging at various ages, and then with conventional echocardiographic analysis and speckle-tracking based strain analyses. RESULTS: With speckle-tracking based strain analyses, diabetic Akita mice showed changes in average global radial strain at the age of 12 weeks, as well as decreased longitudinal strain. These changes occurred in the early stage and remained throughout the progression of diabetic cardiomyopathy in Akita mice. Speckle-tracking showed that the detailed and precise changes of cardiac deformation in the progression of diabetic cardiomyopathy in the genetic type I diabetic Akita mice were uncoupled. CONCLUSIONS: We monitored early-stage changes in the heart of diabetic Akita mice. We utilize this technique to elucidate the underlying mechanism for heart failure in Akita genetic type I diabetic mice. It will further advance the assessment of cardiac abnormalities, as well as the discovery of new drug treatments using Akita genetic type I diabetic mice.
BACKGROUND/AIMS: Diabetes mellitus (DM) has been demonstrated to have a strong association with heart failure. Conventional echocardiographic analysis cannot sensitively monitor cardiac dysfunction in type I diabetic Akita hearts, but the phenotype of heart failure is observed in molecular levels during the early stages. METHODS: Male Akita (Ins2WT/C96Y) mice were monitored with echocardiographic imaging at various ages, and then with conventional echocardiographic analysis and speckle-tracking based strain analyses. RESULTS: With speckle-tracking based strain analyses, diabetic Akitamice showed changes in average global radial strain at the age of 12 weeks, as well as decreased longitudinal strain. These changes occurred in the early stage and remained throughout the progression of diabetic cardiomyopathy in Akita mice. Speckle-tracking showed that the detailed and precise changes of cardiac deformation in the progression of diabetic cardiomyopathy in the genetic type I diabetic Akitamice were uncoupled. CONCLUSIONS: We monitored early-stage changes in the heart of diabetic Akitamice. We utilize this technique to elucidate the underlying mechanism for heart failure in Akita genetic type I diabeticmice. It will further advance the assessment of cardiac abnormalities, as well as the discovery of new drug treatments using Akita genetic type I diabeticmice.
Authors: Lisa C Heather; Anne D Hafstad; Ganesh V Halade; Romain Harmancey; Kimberley M Mellor; Paras K Mishra; Erin E Mulvihill; Miranda Nabben; Michinari Nakamura; Oliver J Rider; Matthieu Ruiz; Adam R Wende; John R Ussher Journal: Am J Physiol Heart Circ Physiol Date: 2022-06-03 Impact factor: 5.125
Authors: Mitchel Tate; Gavin C Higgins; Miles J De Blasio; Runa Lindblom; Darnel Prakoso; Minh Deo; Helen Kiriazis; Min Park; Carlos D Baeza-Garza; Stuart T Caldwell; Richard C Hartley; Thomas Krieg; Michael P Murphy; Melinda T Coughlan; Rebecca H Ritchie Journal: Cardiovasc Drugs Ther Date: 2019-12 Impact factor: 3.727
Authors: Mitchel Tate; Darnel Prakoso; Andrew M Willis; Cheng Peng; Minh Deo; Cheng Xue Qin; Jesse L Walsh; David M Nash; Charles D Cohen; Alex K Rofe; Arpeeta Sharma; Helen Kiriazis; Daniel G Donner; Judy B De Haan; Anna M D Watson; Miles J De Blasio; Rebecca H Ritchie Journal: Front Physiol Date: 2019-11-14 Impact factor: 4.566