Peyman Eini1,2, Maria Shirvani3, Mehrdad Hajilooi4, Farzaneh Esna-Ashari5. 1. Department of Infectious Diseases, Hamadan University of Medical Sciences, Hamadan, Iran. 2. Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. 3. Department of Infectious Diseases, Kermanshah University of Medical Sciences, Kermanshah, Iran. 4. Department of Immunology, Hamadan University of Medical Sciences, Hamadan, Iran. 5. Department of Community and Preventive Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract
BACKGROUND: The inflammatory response to Mycobacterium tuberculosis bacilli influences tuberculosis (TB) progression. In this study, we aimed to identify the Phe206Leu polymorphism and serum L-selectin level in TB patients, compared to healthy individuals. METHODS: Ninety patients with a diagnosis of TB and 90 healthy controls were selected in this study. The serum L-selectin level was determined, using ELISA. L-selectin polymorphism was also evaluated using PCR. For data analysis, SPSS was used at a significance level of 0.05. RESULTS: According to the findings, the mean±SD age of the participants was 57.5 ± 18.4 and 56.5 ± 17.5 years in the TB and healthy groups, respectively. The TB group showed a significantly higher serum L-selectin level (1721.1 ± 330.9) versus the healthy controls (1624 ± 279). The L-selectin Phe allele frequencies were higher than the Leu allele frequencies in the main population, whereas the patients and controls were not significantly different. Eight (0.04%) subjects had Leu/Leu genotypes, 84 (46.6%) carried Phe/Leu genotypes, and 88 (48.8%) had Phe/Phe genotypes. Our results showed that the groups were not significantly different regarding L-selectin genotypes. CONCLUSION: TB patients had a significantly higher serum L-selectin level, compared to the controls. Based on the findings, the incidence of TB and L-selectin polymorphism in the Phe206Leu gene had no significant association.
BACKGROUND: The inflammatory response to Mycobacterium tuberculosis bacilli influences tuberculosis (TB) progression. In this study, we aimed to identify the Phe206Leu polymorphism and serum L-selectin level in TB patients, compared to healthy individuals. METHODS: Ninety patients with a diagnosis of TB and 90 healthy controls were selected in this study. The serum L-selectin level was determined, using ELISA. L-selectin polymorphism was also evaluated using PCR. For data analysis, SPSS was used at a significance level of 0.05. RESULTS: According to the findings, the mean±SD age of the participants was 57.5 ± 18.4 and 56.5 ± 17.5 years in the TB and healthy groups, respectively. The TB group showed a significantly higher serum L-selectin level (1721.1 ± 330.9) versus the healthy controls (1624 ± 279). The L-selectinPhe allele frequencies were higher than the Leu allele frequencies in the main population, whereas the patients and controls were not significantly different. Eight (0.04%) subjects had Leu/Leu genotypes, 84 (46.6%) carried Phe/Leu genotypes, and 88 (48.8%) had Phe/Phe genotypes. Our results showed that the groups were not significantly different regarding L-selectin genotypes. CONCLUSION: TB patients had a significantly higher serum L-selectin level, compared to the controls. Based on the findings, the incidence of TB and L-selectin polymorphism in the Phe206Leu gene had no significant association.
Authors: Guglielmo M Venturi; LiLi Tu; Takafumi Kadono; Adil I Khan; Yoko Fujimoto; Philip Oshel; Cheryl B Bock; Ann S Miller; Ralph M Albrecht; Paul Kubes; Douglas A Steeber; Thomas F Tedder Journal: Immunity Date: 2003-11 Impact factor: 31.745
Authors: Aaron E Hirsh; Anthony G Tsolaki; Kathryn DeRiemer; Marcus W Feldman; Peter M Small Journal: Proc Natl Acad Sci U S A Date: 2004-03-23 Impact factor: 11.205