Khosro Sardarian1, Mohammad Abasi2, Ghasem Solgi3, Mehrdad Hajilooi4. 1. Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. khsardarian@gmail.com. 2. Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. 3. Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. 4. Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. mhajilooi@gmail.com.
Abstract
BACKGROUND: Previous studies revealed that selectins play key roles in homing of immune cells to inflamed tissues and lymphatic organs. L-selectins are expressed on immune cells and interact with P and E selectins to homing to the tissues, hence, the polymorphisms within the gene of L-selectins may are associated with alteration in its expression. Thus, the current cross-sectional analytical study has been designed to investigate the polymorphisms within L-selectin gene and their relation with visceral leishmaniasis (VL). METHODS: This study was performed on 194 samples during 2004-2012.The PCR-SSP and immunoflorescence techniques were used to evaluate the L-selectins polymorphism and anti-Leishmania antibody titration, respectively, in 56, 74 and 64 seropositive VL patients (group 1), seropositive healthy controls (group 2) and seronegative healthy controls (group 3). RESULTS: The results showed that the genotypes (P=0.711) and alleles (P=0.679) within L-selectins gene (A/C) was not differ between groups. Our results also demonstrated that the genotypes within L-selectins in group 1 (P=0.807) and 2 (P=0.441) were not associated with the titration of anti-leishmania antibody. CONCLUSIONS: The results identified that the polymorphisms within L-selectins gene were not associated with VL and it may be concluded that these genotypes and alleles are unable to affect immune responses in VL patients.
BACKGROUND: Previous studies revealed that selectins play key roles in homing of immune cells to inflamed tissues and lymphatic organs. L-selectins are expressed on immune cells and interact with P and E selectins to homing to the tissues, hence, the polymorphisms within the gene of L-selectins may are associated with alteration in its expression. Thus, the current cross-sectional analytical study has been designed to investigate the polymorphisms within L-selectin gene and their relation with visceral leishmaniasis (VL). METHODS: This study was performed on 194 samples during 2004-2012.The PCR-SSP and immunoflorescence techniques were used to evaluate the L-selectins polymorphism and anti-Leishmania antibody titration, respectively, in 56, 74 and 64 seropositive VL patients (group 1), seropositive healthy controls (group 2) and seronegative healthy controls (group 3). RESULTS: The results showed that the genotypes (P=0.711) and alleles (P=0.679) within L-selectins gene (A/C) was not differ between groups. Our results also demonstrated that the genotypes within L-selectins in group 1 (P=0.807) and 2 (P=0.441) were not associated with the titration of anti-leishmania antibody. CONCLUSIONS: The results identified that the polymorphisms within L-selectins gene were not associated with VL and it may be concluded that these genotypes and alleles are unable to affect immune responses in VL patients.