A L Sundell1, C Ullbro2, G Dahlén3, A Marcusson4, S Twetman5. 1. Department of Paediatric Dentistry, Institute for Postgraduate Dental Education, P.O. Box 1030, SE-551 11, Jönköping, Sweden. annalena.sundell@rjl.se. 2. Department of Clinical Dentistry, The Arctic University of Norway, Tromsø, Norway. 3. Department of Oral Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden. 4. Dentofacial Orthopedics, Maxillofacial Unit, Linköping University Hospital, Linköping, Sweden. 5. Department of Odontology, Section for Cariology and Endodontics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Abstract
AIM: To compare the salivary microbial profile in children with oral clefts to matched non-cleft controls in a cross-sectional study. METHODS: The cleft group consisted of 80 children aged 5 years and 144 age-matched non-cleft children were recruited as a control group. Stimulated whole saliva samples were collected and analysed with checkerboard DNA-DNA hybridisation using 12 pre-determined bacterial probes. Background data on caries, oral hygiene, dietary habits and fluoride exposure were collected from clinical examinations and questionnaires. RESULTS: Children in the cleft group had a significantly higher prevalence of dental caries but only minor differences in the salivary microbial profile were found. In general, the cleft-children displayed a lower prevalence of common commensal species (e.g. Streptococcus, Fusobacterium) when compared with the non-cleft controls. The differences were statistically significant (p < 0.05) but the clinical significance was unclear. CONCLUSIONS: The profile of the salivary microflora in 5-year-old children with and without oral clefts was basically similar and displayed only marginal differences with respect to commensal bacteria.
AIM: To compare the salivary microbial profile in children with oral clefts to matched non-cleft controls in a cross-sectional study. METHODS: The cleft group consisted of 80 children aged 5 years and 144 age-matched non-cleft children were recruited as a control group. Stimulated whole saliva samples were collected and analysed with checkerboard DNA-DNA hybridisation using 12 pre-determined bacterial probes. Background data on caries, oral hygiene, dietary habits and fluoride exposure were collected from clinical examinations and questionnaires. RESULTS:Children in the cleft group had a significantly higher prevalence of dental caries but only minor differences in the salivary microbial profile were found. In general, the cleft-children displayed a lower prevalence of common commensal species (e.g. Streptococcus, Fusobacterium) when compared with the non-cleft controls. The differences were statistically significant (p < 0.05) but the clinical significance was unclear. CONCLUSIONS: The profile of the salivary microflora in 5-year-old children with and without oral clefts was basically similar and displayed only marginal differences with respect to commensal bacteria.
Authors: Floyd E Dewhirst; Tuste Chen; Jacques Izard; Bruce J Paster; Anne C R Tanner; Wen-Han Yu; Abirami Lakshmanan; William G Wade Journal: J Bacteriol Date: 2010-07-23 Impact factor: 3.490
Authors: Jørn A Aas; Ann L Griffen; Sara R Dardis; Alice M Lee; Ingar Olsen; Floyd E Dewhirst; Eugene J Leys; Bruce J Paster Journal: J Clin Microbiol Date: 2008-01-23 Impact factor: 5.948