Leonardo Santos Antunes1, Patricia Nivoloni Tannure2, Lívia Azeredo Alves Antunes1, Maria Fernanda Reis3, Marcelo Castro Costa4, Cresus Vinicius Depes Gouvêa5, Beni Olej6, Jose Mauro Granjeiro7, Erika Calvano Küchler8. 1. Assistant Professor, Department of Specific Formation, School of Dentistry Fluminense Federal University, Niterói, RJ, Brazil. 2. Professor, Discipline of Pediatric Dentistry, School of Dentistry, Veiga de Almeida University, RJ, Brazil. 3. Undergraduate Student, Unit of Clinical Research, Fluminense Federal University Niterói, RJ, Brazil. 4. Associate Professor, Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, RJ, Brazil. 5. Chairman, Department of Prosthodontics, School of Dentistry, Fluminense Federal University, Niterói, RJ, Brazil. 6. Head and Associate Professor, Cell Therapy Center, Unit of Clinical Research Fluminense Federal University, Niterói, RJ, Brazil. 7. Head and Senior Researcher, National Institute of Metrology, Quality and Technology Bioengineering Program, Cell Therapy Center Unit of Clinical Research, Fluminense Federal University Niterói, Rio de Janeiro, Brazil. 8. Postdoctrol, Cell Therapy Center, Unit of Clinical Research, Fluminense Federal University, Niterói, RJ, Brazil e-mail: erikacalvano@gmail.com.
Abstract
AIM: To evaluate the association of the polymorphisms in the TGFB3 gene (rs2268626), and the BMP4 gene (rs17563) with dental caries in two different groups (noncleft group and oral cleft group) from a cleft center located at Rio de Janeiro, Brazil. MATERIALS AND METHODS: A total of 486 unrelated children and adolescents with or without caries were evaluated using a cohort design. Data on oral health habits was obtained through a questionnaire and caries data was collected by clinical examination. Genotyping of the selected polymorphisms for TGFB3 and BMP4 were carried out by real-time PCR using the TaqMan assay method from a genomic DNA isolated from buccal epithelial cells of all children and adolescents. RESULTS: No association was found between BMP4 polymorphism and caries among individuals from both groups. For TGFB3 polymorphism, significant differences were observed for allele and genotype frequencies between caries free and caries affected individuals in oral cleft group (p = 0.013 and 0.006 for allele and genotype frequencies respectively). CONCLUSION: Our findings provide evidence suggesting that TGFB3 may be involved in caries susceptibility in oral cleft group. CLINICAL SIGNIFICANCE: In the future, the possibility of identifying genes related to caries susceptibility can lead to counseling of the individual that carries gene alterations, with the aim of working on preventive measures for caries as well as bioengineering treatments.
AIM: To evaluate the association of the polymorphisms in the TGFB3 gene (rs2268626), and the BMP4 gene (rs17563) with dental caries in two different groups (noncleft group and oral cleft group) from a cleft center located at Rio de Janeiro, Brazil. MATERIALS AND METHODS: A total of 486 unrelated children and adolescents with or without caries were evaluated using a cohort design. Data on oral health habits was obtained through a questionnaire and caries data was collected by clinical examination. Genotyping of the selected polymorphisms for TGFB3 and BMP4 were carried out by real-time PCR using the TaqMan assay method from a genomic DNA isolated from buccal epithelial cells of all children and adolescents. RESULTS: No association was found between BMP4 polymorphism and caries among individuals from both groups. For TGFB3 polymorphism, significant differences were observed for allele and genotype frequencies between caries free and caries affected individuals in oral cleft group (p = 0.013 and 0.006 for allele and genotype frequencies respectively). CONCLUSION: Our findings provide evidence suggesting that TGFB3 may be involved in caries susceptibility in oral cleft group. CLINICAL SIGNIFICANCE: In the future, the possibility of identifying genes related to caries susceptibility can lead to counseling of the individual that carries gene alterations, with the aim of working on preventive measures for caries as well as bioengineering treatments.