Literature DB >> 293672

Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.

B Wold, M Wigler, E Lacy, T Maniatis, S Silverstein, R Axel.   

Abstract

The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 293672      PMCID： PMC411714          DOI： 10.1073/pnas.76.11.5684

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.

Authors:  S KIT; D R DUBBS; L J PIEKARSKI; T C HSU
Journal:  Exp Cell Res       Date:  1963-08       Impact factor: 3.905

2.  Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors:  M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

3.  Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors:  M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

4.  Characterization of the ribosome-protected regions of 125I-labelled rabbit globin messenger RNA.

Authors:  S Legon
Journal:  J Mol Biol       Date:  1976-09-05       Impact factor: 5.469

5.  Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome.

Authors:  R C Mulligan; B H Howard; P Berg
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

6.  The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.

Authors:  A Efstratiadis; F C Kafatos; T Maniatis
Journal:  Cell       Date:  1977-04       Impact factor: 41.582

7.  Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.

Authors:  J C Myers; S Spiegelman
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

8.  The isolation of structural genes from libraries of eucaryotic DNA.

Authors:  T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

9.  Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA.

Authors:  N J Maitland; J K McDougall
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

10.  Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA.

Authors:  N Mantei; W Boll; C Weissmann
Journal:  Nature       Date:  1979-09-06       Impact factor: 49.962
View more
  42 in total

1.  High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.

Authors:  R M Sandri-Goldin; A L Goldin; M Levine; J C Glorioso
Journal:  Mol Cell Biol       Date:  1981-08       Impact factor: 4.272

2.  Gene transfer of truncated NGF receptor clones leads to cell surface expression in mouse fibroblasts.

Authors:  A Sehgal; M Bothwell; M Chao
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

3.  A combination of RNase H and S1 nuclease circumvents an artefact inherent to conventional S1 analysis of RNA splicing.

Authors:  S S Sisodia; D W Cleveland; B Sollner-Webb
Journal:  Nucleic Acids Res       Date:  1987-03-11       Impact factor: 16.971

4.  On the road from classical to modern molecular biology.

Authors:  Tom Maniatis
Journal:  Nat Med       Date:  2012-10       Impact factor: 53.440

5.  High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.

Authors:  D DiMaio; V Corbin; E Sibley; T Maniatis
Journal:  Mol Cell Biol       Date:  1984-02       Impact factor: 4.272

6.  The construction of cosmid libraries which can be used to transform eukaryotic cells.

Authors:  F G Grosveld; T Lund; E J Murray; A L Mellor; H H Dahl; R A Flavell
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

7.  DNA-mediated gene transfer in Friend leukemia cells by cotransfection of simian virus 40 DNA with herpes simplex virus thymidine kinase DNA.

Authors:  G Steinheider; I Greiser-Wilke; H Hauser; C Bumke-Vogt; K Moelling; A Graessmann
Journal:  J Virol       Date:  1983-01       Impact factor: 5.103

8.  Construction and use of a dominant, selectable marker: a Harvey sarcoma virus-dihydrofolate reductase chimera.

Authors:  M J Murray; R J Kaufman; S A Latt; R A Weinberg
Journal:  Mol Cell Biol       Date:  1983-01       Impact factor: 4.272

Review 9.  The molecular basis of disorders of human hemoglobin synthesis.

Authors:  F Ramirez; J G Mears; A Bank
Journal:  Mol Cell Biochem       Date:  1980-08-16       Impact factor: 3.396

10.  Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells.

Authors:  N Hsiung; H Warrick; J K deRiel; D Tuan; B G Forget; A Skoultchi; R Kucherlapati
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.