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Literature DB >> 6294612

The construction of cosmid libraries which can be used to transform eukaryotic cells.

F G Grosveld, T Lund, E J Murray, A L Mellor, H H Dahl, R A Flavell.

Abstract

Cosmid vectors have been developed which carry selective markers for growth in bacteria (beta lactamase gene) and animal cells (the Herpes Simplex virus thymidine kinase gene, the transposon Tn-5 aminoglycosyl 3' phosphotransferase gene and the E. coli guanine phosphoribosyltransferase gene). The design of the cosmids allows the exchange of the eukaryotic markers in recombinant cosmids. Human and mouse cosmid libraries containing DNA inserts of about 40kb have been generated by an improved method. Several clones from the human beta-globin locus were isolated. These cosmids transform mouse L cells at high efficiency in both circular and linear form. The newly introduced genes are expressed accurately in L cells.

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Year: 1982 PMID： 6294612 PMCID： PMC326959 DOI： 10.1093/nar/10.21.6715

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

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3. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
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4. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

Authors: R F Weaver; C Weissmann
Journal: Nucleic Acids Res Date: 1979-11-10 Impact factor: 16.971

5. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.

Authors: V M Vogt
Journal: Eur J Biochem Date: 1973-02-15

6. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

7. An approach to evolutionary relationships of mammalian DNA viruses through analysis of the pattern of nearest neighbor base sequences.

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8. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.

Authors: P A Weil; D S Luse; J Segall; R G Roeder
Journal: Cell Date: 1979-10 Impact factor: 41.582

9. Purified DNAs are transcribed after microinjection into Xenopus oocytes.

Authors: J E Mertz; J B Gurdon
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

10. Transcription of xenopus tDNAmet1 and sea urchin histone DNA injected into the Xenopus oocyte nucleus.

Authors: A Kressmann; S G Clarkson; J L Telford; M L Birnstiel
Journal: Cold Spring Harb Symp Quant Biol Date: 1978

132 in total

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5. Isolation and characterization of the gene encoding the testis specific histone protein H2B-2 from the sea urchin Lytechinus pictus.

Authors: Z C Lai; G Childs
Journal: Nucleic Acids Res Date: 1986-09-11 Impact factor: 16.971

10. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db.

Authors: H Allen; J Fraser; D Flyer; S Calvin; R Flavell
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