R J Wierichs1,2, S Westphal3, J Lausch3, H Meyer-Lueckel3,4, M Esteves-Oliveira3. 1. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany. rwierichs@ukaachen.de. 2. Department of Biohybrid & Medical Textiles, Institute of Applied Medical Engineering, RWTH Aachen University, Aachen, Germany. rwierichs@ukaachen.de. 3. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany. 4. Department of Restorative, Preventive and Pediatric Dentistry, zmk bern, University of Bern, Bern, Switzerland.
Abstract
OBJECTIVES: The aim of this in vitro study was to evaluate the role of highly fluoridated dentifrice on remineralization characteristics of lowly and highly pre-demineralized enamel artificial caries lesions. METHODS: Bovine enamel specimens were prepared (pH 4.95; 21 days) and discriminated in either lowly [L] or highly [H] pre-demineralized artificial caries lesions. Specimens with a mean ΔZbaseline,L (95% CI) of 5120 (4995; 5245) vol.% × μm and a mean ΔZbaseline,H of 8187 (8036; 8339) vol.% × μm were selected and randomly allocated to 12 groups (n = 20). Treatments during pH-cycling (28 days; 6 × 60 min demineralization/day) were brushing 2×/day with fluoride-free (0 ppm F- [L0/H0]), 1100 ppm F- [L1100/H1100], 2800 ppm F- [L2800/H2800], 5000 ppm F- [L5000/H5000], 5000 ppm F- + glycerin [L5000 + glycerin/H5000 + glycerin], and 5000 ppm F- + TCP [L5000 + TCP/H5000 + TCP] containing dentifrices. Dentifrice slurries were prepared with deionized water (1:3wt/wt). After cycling specimens presenting lesion surface loss were discarded and for the remaining 202 specimens, transversal microradiographic (TMR) analyses (ΔZpH-cycle/LDpH-cycle) were performed again. Changes in mineral loss (ΔΔZ = ΔZbaseline - ΔZpH-cycle) and lesion depth (ΔLD = LDbaseline - LDpH-cycle) were calculated. RESULTS: Significant differences for ΔΔZ could be found between L0, L1100, and L5000 as well as H0, H1100, and H2800/H5000 (p ≤ 0.01; ANCOVA). Except for 0 ppm F-, higher ΔΔZ could be found in highly compared with lowly demineralized specimens (p ≤ 0.004; ANCOVA). After pH-cycling, a second lesion front could only be observed in H5000 and H5000 + TCP. The correlation between ΔΔZ and F- was moderate for lowly and highly demineralized lesions (rL = 0.591; pL < 0.001; rH = 0.746; pH < 0.001), indicating a fluoride dose response for both. CONCLUSION: For both baseline substrate conditions, a dose response for fluoride could be revealed. CLINICAL SIGNIFICANCE: Remineralization characteristics of enamel directly depended on baseline mineral loss.
OBJECTIVES: The aim of this in vitro study was to evaluate the role of highly fluoridated dentifrice on remineralization characteristics of lowly and highly pre-demineralized enamel artificial caries lesions. METHODS:Bovine enamel specimens were prepared (pH 4.95; 21 days) and discriminated in either lowly [L] or highly [H] pre-demineralized artificial caries lesions. Specimens with a mean ΔZbaseline,L (95% CI) of 5120 (4995; 5245) vol.% × μm and a mean ΔZbaseline,H of 8187 (8036; 8339) vol.% × μm were selected and randomly allocated to 12 groups (n = 20). Treatments during pH-cycling (28 days; 6 × 60 min demineralization/day) were brushing 2×/day with fluoride-free (0 ppm F- [L0/H0]), 1100 ppm F- [L1100/H1100], 2800 ppm F- [L2800/H2800], 5000 ppm F- [L5000/H5000], 5000 ppm F- + glycerin [L5000 + glycerin/H5000 + glycerin], and 5000 ppm F- + TCP [L5000 + TCP/H5000 + TCP] containing dentifrices. Dentifrice slurries were prepared with deionized water (1:3wt/wt). After cycling specimens presenting lesion surface loss were discarded and for the remaining 202 specimens, transversal microradiographic (TMR) analyses (ΔZpH-cycle/LDpH-cycle) were performed again. Changes in mineral loss (ΔΔZ = ΔZbaseline - ΔZpH-cycle) and lesion depth (ΔLD = LDbaseline - LDpH-cycle) were calculated. RESULTS: Significant differences for ΔΔZ could be found between L0, L1100, and L5000 as well as H0, H1100, and H2800/H5000 (p ≤ 0.01; ANCOVA). Except for 0 ppm F-, higher ΔΔZ could be found in highly compared with lowly demineralized specimens (p ≤ 0.004; ANCOVA). After pH-cycling, a second lesion front could only be observed in H5000 and H5000 + TCP. The correlation between ΔΔZ and F- was moderate for lowly and highly demineralized lesions (rL = 0.591; pL < 0.001; rH = 0.746; pH < 0.001), indicating a fluoride dose response for both. CONCLUSION: For both baseline substrate conditions, a dose response for fluoride could be revealed. CLINICAL SIGNIFICANCE: Remineralization characteristics of enamel directly depended on baseline mineral loss.
Authors: M Esteves-Oliveira; N M Santos; H Meyer-Lueckel; R J Wierichs; J A Rodrigues Journal: Clin Oral Investig Date: 2016-03-19 Impact factor: 3.573
Authors: Richard Johannes Wierichs; Judith Mester; Thomas Gerhard Wolf; Hendrik Meyer-Lueckel; Marcella Esteves-Oliveira Journal: Clin Oral Investig Date: 2021-11-24 Impact factor: 3.573
Authors: Ellen Elisabeth Jansen; Hendrik Meyer-Lueckel; Marcella Esteves-Oliveira; Richard Johannes Wierichs Journal: Clin Oral Investig Date: 2020-12-14 Impact factor: 3.573